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ARS Home » Research » Publications at this Location » Publication #96730
Title: AFLP AND RAPD MARKERS LINKED TO PHYTOPHTHORA FRAGARIAE RESISTANCE GENES IN STRAWBERRY

Author
item HAYMES, KENNETH - CONTRACT EMPLOYEE
item Hokanson, Stan
item VAN DER WEG, W. - WAGENINGEN, NETHERLANDS
item Maas, John

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/26/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Strawberry (Fragaria x ananassa) is susceptible to the red stele root rot dissease caused by the fungus Phytophthora fragariae var. fragariae. Red stele resistance in strawberries and virulence of P. fragariae behave according to the gene-for-gene system. Resistance genes and their corresponding avirulence genes have been identified by screening plant roots for infection. The goal of this research project is to develop highly specific molecular markers for four Rpf resistance genes (Rpf1, Rpf2, Rpf3, and Rpf6) that confer resistance against P. fragariae. Bulk segregant analysis was used to identify RAPD and AFLP markers putatively linked to P. fragariae resistance genes. The bulked DNAs representing subsets of three F1 populations that segregated monogenically for either resistance or susceptibility to P. fragariae. Markers have been shown to be tightly linked to Rpf1, Rpf3, and Rpf6 genes ranging from 0 cM to 7 cM and 12.5 cM from Rpf2. The RAPD and AFLP markers linked to the resistance genes are being converted into SCAR markers for the detection of P. fragariae. The application of the molecular markers linked to resistance genes will have direct applicability to strawberry breeding programs and to the strawberry industry worldwide. Marker-facilitated selection of these resistance genes would allow an efficient means in the screening and selection of plant material containing these genes and help in the pyramiding for resistance to P. fragariae.