IDENTIFICATION OF AN AFLP MARKER LINKED TO THE BETA-MODIFIER (MO-B) LOCUS AND DEVELOPMENT OF SCAR MARKERS LINKED TO THE BETA (B) AND MO-B LOCI IN TOMATO

Authors: Zhang, Yiping; Stommel, John

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Expression of the Beta (B) locus in tomato increases fruit
beta-carotene content at the expense of lycopene and results
in increased orange fruit pigmentation. The degree to which B
is expressed is influenced by the beta-modifier (Mo-B) gene
which segregates independently of B. Amplified fragment
length polymorphism (AFLP) analysis was performed using a pair
of near isogenic lines (NILs) designed to be isogenic at the B
locus. NILs were derived from an initial cross of
Lycopersicon esculentum cv. Rutgers x L. hirsutum. Using 64
AFLP primer pairs, polymorphic fragments were identified which
distinguished the NILs. A 100 bp AFLP amplification product
was identified which was unique to the red-fruited NIL.
Analysis of an F-2 population derived from a cross of the NILs
confirmed that the 100 bp product cosegregated with MoB and
was tightly linked to this locus. The marker exhibited a
recombination frequency of 1.7%. This MoB-linked AFLP marker
was cloned and sequenced. The sequences were used to design
24-mer oligonucleotide primers for respective clones and
develop sequence characterized amplified region (SCAR) markers
for use in marker-aided selection, high-resolution linkage
analysis, and map-based cloning.