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ARS Home » Research » Publications at this Location » Publication #97806
Title: INFLUENCE OF PLANT GROWTH REGULATORS AND LIGHT LEVELS ON SHOOT MORPHOGENESIS FROM LEAF EXPLANTS OF HIGHBRUSH BLUEBERRY.

Author
item CAO, XIAOLING - CONTRACT EMPLOYEE
item Hammerschlag, Freddi

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 7/31/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: As part of a program to develop transgenic highbush blueberry (Vaccinium corymbosum L.) cultivars, studies were conducted to determine optimum conditions for high efficiency shoot regeneration from leaf explants of in vitro propagated shoot cultures. The effect of either thidiazuron at 1 or 5 uM , or zeatin riboside at 20 uM, and two light levels (18 or 55 umol per meter per second) on shoot organogenesis were investigated. With the exception of Bluecrop, which did not regenerate shoots, maximum shoot regeneration of 13, 12.7, 12.6 and 4.6 shoots per explant for cultivars Duke, Georgiagem, Sierra and Jersey, respectively, occurred on regeneration medium with zeatin riboside and under a light intensity of 55 umol per meter per second. Whereas Duke regenerated equally well on regeneration medium with either zeatin riboside or 5 uM thidiazuron, regeneration frequencies for Georgiagem and Sierra were significantly higher on zeatin riboside. A light intensity of 55 umol per meter per second significantly increased regeneration of cultivars Duke, Jersey, and Sierra on zeatin riboside, but inhibited regeneration of Duke on 5 uM thidazuron.