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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #98087
Title: EXPRESSION OF TRANSCRIPTS FOR TRANSCRIPTION FACTORS AND ADIPOCYTE-RELATED PROTEINS IN NEONATAL PIGS

Author
item DING, SHIH-TORNG - BAYLOR COLLEGE OF MED
item MCNEEL, RONALD - BAYLOR COLLEGE OF MED
item Mersmann, Harry

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: An interpretive summary is not required for this abstract.

Technical Abstract: Several transcription factors form a cascade to regulate each other and adipocyte differentiation. We studied the expression of adipocyte transcripts during development of subcutaneous porcine adipose tissue. Crossbred pigs were 0, 3, 10, 17 and 28 days old. Cloned porcine gene fragments were used to make riboprobes for northern analysis (20 mug total RNA/lane). The developmental patterns for peroxisome proliferator-activate receptor gamma (PPAR gamma), adipocyte determination and differentiation- dependent factor 1 (ADD1), and lipoprotein lipase (LPL) were similar. Each transcript began at low concentration and increased steadily, about 10-fold to day 28. These results suggest that PPAR gamma and ADD1 regulate the development of LPL in porcine adipocytes. The transcripts for CCAAT/enhancer binding protein alpha (C/EBP alpha) were at low levels until a 10-fold increase at day 28. Transcripts for retinoid x receptor alpha and dadipocyte fatty acid binding protein (aP2) were at >50% of day 28 levels a all earlier ages. The high aP2 and low C/EBP alpha transcript levels were unexpected because the levels for both were low early in differentiation and increased during differentiation of rodent-derived clonal adipocytes and porcine stromal/vascular cells in vitro.