CHROMOSOME LANDING AND SEQUENCE ANALYSIS OF THE MLA RESISTANCE GENE CLUSTERIN BARLEY

Authors: Wise, Roger; WEI, FUSHENG - IOWA STATE UNIVERSITY; MORROLL, SHAUN - FORMER USDA EMPLOYEE; KURTH, JOACHIM - SAINSBURY LABORATORY; Gobelman Werner, Karin; MAO, LONG - CLEMSON UNIVERSITY; WOO, SUNG - CLEMSON UNIVERSITY; SCHULZE-LEFERT, PAUL - SAINSBURY LABORATORY; WING, ROD - CLEMSON UNIVERSITY

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/25/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Powdery mildew of barley, caused by Erysiphe graminis f. sp. hordei, is a model system for investigating the mechanisms of gene-for-gene interaction between large-genome cereals and obligate-fungal pathogens. A large number of loci that confer resistance to this disease are located on the short arm of chromosomes 5(1H). The Mla resistance-gene cluster is positioned near the telomeric end and this chromosome arm. We have saturated the Mla region with AFLP, RAPD, and RFLP markers and have used these markers to identify and develop a contig of YAC and BAC clones spanning the Mla cluster. Two overlapping BACs totaling 150-kb were selected for high- throughput sequencing. Computational analysis via BLAST surveys suggests that there are at least two NBS-LRR resistance gene analogs (RGA), a chymotrypsin inhibitor gene family, several BARE-1 copia-like retro elements, and an acyl carrier protein III among this gene rich region. PCR Rprimers are being developed to genetically position the gene-like sequence on our high-resolution recombinant population.