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ARS Home » Research » Publications at this Location » Publication #99742
Title: CHARACTERIZATION OF A NEW ADENOVIRUS SPECIES ISOLATED FROM BLACK-TAILED DEER IN CALIFORNIA

Author
item LEHMKUHL, HOWARD
item WOODS, LESLIE - UNIV.OF CALIF.,DAVIS, CA

Submitted to: International Congress of Virology
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: An adenovirus associated with systemic and localized vascular damage was demonstrated by transmission electron microscopy and immunohistochemistry in a newly recognized epizootic hemorrhagic disease in California black-tailed deer. Currently in ruminants, there are 10 bovine adenovirus (BAdV), 2 caprine (GAdV) and 6 ovine (OAdV) species. The objectives of this study were to describe the cultural, physicochemical and serological characteristics of a virus isolated from a deer lung from a field case using fetal deer cell cultures. Infected cells were refractile eventually became swollen and rounded. Single intranuclear inclusions were sometimes evident early in the infection cycle. The virus would not replicate in low passage bovine, caprine or ovine cells. The virus had the cultural, morphological and physicochemical characteristics of members of the Adenoviridae family. Antiserum to the deer adenovirus (homologous titer 262,144) neutralized BAdV 6 and 7 and GAdV 1 (heterologous titer 512, 128 and 16,384 respectively). Antiserum to BAdV 6 (homologous titer 2,048) did not neutralize the deer adenovirus but antiserum to BAdV 7 (homologous titer 8,192) and GAdV 1 (homologous titer 32,768) neutralized the deer adenovirus (heterologous titer for both 16,384). Cross-neutralization with the other BAdV, GAdV and OAdV, species was not observed. Restriction endonuclease patterns generated for the deer adenovirus were unique. While the deer adenovirus was closely related antigenically to BAdV 7 and GAdV 1, it appears sufficiently distinct culturally, and molecularly to justify its consideration as a new adenovirus.