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Research Project: Conservation and Utilization of Temperate-Adapted Fruit, Nut, and Other Specialty Crop Genetic Resources

Location: National Clonal Germplasm Repository

Title: A public mid-density genotyping platform for cultivated blueberry

Author
item ZHAO, DONGYAN - Cornell University
item SABKOTA, MANOJ - Cornell University
item GLAUBITZ, JEFF - Cornell University
item Bassil, Nahla
item MENGIST, MOLLA - North Carolina State University
item IORIZZO, MASSIMO - North Carolina State University
item HELLER-USZYNSKA, KASIA - Diversity Arrays Technology
item MOLLINARI, MARCELO - North Carolina State University
item BEIL, CRAIG - Cornell University
item SHEEHAN, MOIRA - Cornell University

Submitted to: Genetic Resources and Crop Evolution
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/2/2024
Publication Date: 4/11/2024
Citation: Zhao, D., Sabkota, M., Glaubitz, J., Bassil, N.V., Mengist, M., Iorizzo, M., Heller-Uszynska, K., Mollinari, M., Beil, C., Sheehan, M. 2024. A public mid-density genotyping platform for cultivated blueberry. Genetic Resources and Crop Evolution. 5(9):36-44. https://doi.org/10.46265/genresj.WQZS1824.
DOI: https://doi.org/10.46265/genresj.WQZS1824

Interpretive Summary: In this study, we report the creation of a panel of 3,000 loci distributed across the cultivated blueberry genome for use in molecular breeding and genomic prediction. This marker panel brings cost-effective and rapid genotyping capabilities to public and private breeding programs. The open access provided by this platform will allow genetic data sets generated on the marker panel to be compared and joined across projects, institutions, and countries. This genotyping resource has the power to make routine genotyping a reality for any blueberry breeder.

Technical Abstract: Small public breeding programs have many barriers to adopting technology, particularly creating, and using genetic marker panels for genomic-based decisions in selection. Here we report the creation of a DArTag panel of 3,000 loci distributed across the tetraploid blueberry genome for use in molecular breeding and genomic prediction. The creation of this marker panel brings cost-effective and rapid genotyping capabilities to public and private breeding programs. The open access provided by this platform will allow genetic data sets generated on the marker panel to be compared and joined across projects, institutions, and countries. This genotyping resource has the power to make routine genotyping a reality for any breeder of blueberry.