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Research Project: Improving Dairy Cow Feed Efficiency and Environmental Sustainability Using Genomics and Novel Technologies to Identify Physiological Contributions and Adaptations

Location: Animal Genomics and Improvement Laboratory

Title: Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment

Author
item KRAMER, ASHLEY - University Of Delaware
item ELLWOOD, KATHRYN - University Of Delaware
item GUARINA, NICOLE - University Of Delaware
item Li, Congjun - Cj
item DUTTA, ADITYA - University Of Delaware

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/9/2024
Publication Date: 11/13/2024
Citation: Kramer, A.E., Ellwood, K., Guarina, N., Li, C., Dutta, A. 2024. Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment. Poultry Science. https://doi.org/10.1016/j.psj.2024.104547.
DOI: https://doi.org/10.1016/j.psj.2024.104547

Interpretive Summary: This study is to explore the differential gene expression patterns and unstream regulators in pre-recruitment follicles. The results highlights the dynamic regulatory role of TGGbata1, and network of SMAD3, TNF and TP53 genes. These findings also imply that MYC plays a vital role as a key upstream regulator.

Technical Abstract: Exploring differential gene expression patterns and upstream regulators in pre-recruitment follicles and the F5 pre-ovulatory follicle provides valuable insights into the complex mechanisms that govern follicular development in laying hens. This work highlights the dynamic regulatory role of TGFß1, which orchestrates a network involving SMAD3, TNF, and TP53 in both pre-recruitment and pre-ovulatory follicles. SMAD3 was observed to contribute to growth and differentiation, TNF facilitated cell proliferation, and TP53 regulated cell cycle proliferation. Furthermore, MYC was found to influence several critical genes - CTNNB1, TCF, and TP53, which collectively support cell proliferation and differentiation within pre-ovulatory follicles. These findings imply that MYC plays a vital role as a key upstream regulator in pre-ovulatory follicles.