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OTUshuff

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OTUshuff is a set of tools written in Perl to identify and correct for the presence of pseudo •-diversity in contrasts between microbial communities. The variably weighted Odum dissimilarity (D_wOdum) allows for down-weighting the influence of either abundant or rare taxa in calculating a measure of similarity between two communities. We show that down-weighting the influence of rare taxa can be used to minimize pseudo b-diversity arising from incomplete sampling. Down-weighting the influence of abundant taxa can increase the sensitivity of hypothesis testing. OTUshuff is an associated test for identifying the presence of pseudo •-diversity in pairwise community contrasts.

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OMB 0518 - 0032 (01/2025)

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