Biochemistry

Objectives

• Determine optimum duration of reduced irrigation without significant yield loss
• Compare plant response to different drought tolerance/avoidance strategies
• Determine biochemical responses(s) associated with specific plant physiological response(s)
• Determine gene expression patterns in specific biochemical pathways or proteins and correlate with plants' performance and yield
• Incorporate knowledge of specific biochemical and gene expression patterns to develop molecular markers for the development of drought tolerant/resistant peanut varieties

50 day Control with 100% irrigation	50 day Control with 50% irrigation
Early season water deficit (up to 50 days after germination) provides some drought tolerant benefits.

Biochemical and Gene Expression Study of Peanut TSWV Resistance

Objectives

• Determine the levels of Tomato Spotted Wilt Virus infection using molecular diagnosis
• Correlate peanut TSWV symptoms to the number of viral particles
• Determine biochemical and gene expression patterns of plant responses to the degrees of viral infection
• Correlate specific peanut proteins and genes to the degrees of viral resistance
• Correlate viral resistance in different peanut varieties and determine specific peanut proteins and gene expression patterns
• Incorporate understanding of specific biochemical and gene expression patterns to develop molecular markers for TSWV tolerance/resistance in peanut

Tomato Spotted Wilt Virus (TSWV) infected peanut and PCR diagnosis

Application of Genomics to Understand Peanut Drought Response

• Utilize peanut micro-arrays to determine gene expression patterns in drought resistant versus drought susceptible peanut varieties
• Determine specific regulated proteins or genes that may be involved in drought tolerance
• Develop gene expression profiles on specific proteins or genes using controlled experiments
• Incorporate information on newly discovered proteins or genes to develop moleculamarkers for drought tolerance/resistance in peanut
   
  

  Genomic application involves gene chip micro-array, molecular marker development and variety genetic analysis

 

For more information contact Phat Dang.