Alden Estep

Research Entomologist

CONTACT USDA ARS CMAVE Mosquito & Fly Research Unit 1700 SW 23rd Drive Bldg 1, Room 145 Phone: (352) 374-5906 ORCID ¦ Google Scholar ¦ Research Gate

RESEARCH OVERVIEW

Collaborative research with three foci; the genetic and phenotypic expression of insecticide resistance in vectors of public health disease, the development of novel chemical and genetic toxicants for improved vector control, and the development of novel platforms for rapid assessment and characterization of pathogens and vectors in low resource conditions.

 

LAB MEMBERS, ALUMNI & VISITORS

Neil Sanscrainte (USDA Molecular Biologist)	Lauren Maestas (2020 - 2021)	James Becnel (USDA Retired - 2019)

 

PROFESSIONAL SERVICE

Online managing editor, Journal of the Florida Mosquito Control Association, 2022-present

Chair, Research Selection Subcommittee, Florida Coordinating Council on Mosquito Control, 2021-present

Member, Spatial Repellent Working Group, Armed Forces Pest Management Board, 2022

Member, Research Selection Subcommittee, Florida Coordinating Council on Mosquito Control, 2019-2021

Subject Matter Expert- Entomology, Vector Issues Team, CDC Zika Emergency Operations Center, 2016

Subject Matter Expert- Insecticide Resistance, FL Department of Agriculture and Consumer Services Zika Response, 2016

 

RECENT PRESENTATIONS

2024_Dodd_Module1_History_of_Insecticide_Resistance

2024_Dodd_Module2_Current_State_of_IR

2024_Dodd_Module3_OPerational_Responses_to_IR

 

PATENTS

US9796975- Double-stranded ribonucleic acid as control against insects (mosquitoes)

US10351850- Double-stranded ribonucleic acid as control against insects (house fly)

 

PUBLICATIONS

Ribeiro, V.P.; Bastos, J.K.; Estep, A.S. & Meepagala, K.M. 2024. Larvicidal activity of constituents from the main Brazilian Propolis Types: Green, Red, and Brown against Aedes aegypti.  ACS Omega 9(33), 2024. pp. 35560-35566. https://doi.org/10.1021/acsomega.4c03132

Singh, R., Sanscrainte, N.D., Estep, A.S., González, K. and Bernal, X.E., 2024. Rearing and Shipping of Uranotaenia lowii, a Frog-Biting Mosquito. Bio-protocol, 14(11): e4996. https://doi.org/10.21769/BioProtoc.4996

Estep, A.S., Sanscrainte, N.D., Stuck, J., Unlu, I., Prasauskas, A., Mundis, S.J., Cotter, N., Romero-Weaver, A.L., Fedirko, T.J., Kendziorski, N.L., Kosinski, K.J., Ramirez, D. & Buckner, E.A. 2024. The L1014F Knockdown Resistance Mutation Is Not a Strong Correlate of Phenotypic Resistance to Pyrethroids in Florida Populations of Culex quinquefasciatus. Insects, 15(3), p.197. 2024. https://doi.org/10.3390/insects15030197

Estep, A.S. & Sanscrainte, N.D. 2024. A critical review of insecticide resistance in US Aedes albopictus: Resistance status, underlying mechanisms, and directions for future research. J. Florida Mosquito Control Assoc. 71(1)

Estep, A.S.; Sanscrainte, N.D. & Okech, B.A. Aedes aegypti knockdown resistance mutations and dengue virus infection in Haiti. J. American Mosquito Control Assoc. 40(2): 102–108. 2024. https://doi.org/10.2987/23-7160

Unlu, I.; Buckner, E.A.; Medina, J.; Vasquez, C.; Cabrera, A. & Estep, A.S. Insecticide resistance of Miami-Dade Culex quinquefasciatus populations and initial field efficacy of a new resistance-breaking formulation. PLOS ONE 19(2), p.e0296046. 2024. https://doi.org/10.1371/journal.pone.0296046

Burgess, E.R., IV; Sanscrainte, N.D.; Taylor, C.E.; Buss, L.J.; Estep, A.S. 2023. Expression, activity, and consequences of biochemical inhibition of α- and β-glucosidases in different life stages of Culex quinquefasciatus. PLOS ONE 18(8): e0286609. 2023 https://doi.org/10.1371/journal.pone.0286609

Castro-Llanos, F., Flores-Mendoza, C., Escobedo-Vargas, K., Lozano, M., Zorrilla, V.O., Espada, L., Lopez-Sifuentes, V., Astete, H., Fisher, M.L., Larson, R.L., Vásquez, G.M., Estep, A.S. Implementation of insecticide resistance testing into an existing mosquito-borne pathogen surveillance program in Perú. WingBeats, Spring 2023.

Meepagala, K.M.; Estep, A.S. 2023. Larvicidal constituents from Poncirus trifoliata root extracts. J. Medical Entomology. 2023 Jul6:tjad086. https://doi.org/10.1093/jme/tjad086

Estep, A.S.; Kissoon, K; Saldana, M; & Fredregill, C. 2023. Persistent variation in insecticide resistance intensity in container breeding Aedes (Diptera: Culicidae) co-collected in Houston, TX. J. Medical Entomology. 2023 May 20: tjad051. https://doi.org/10.1093/jme/tjad051

Estep, A.S. Getting real about resistance: The top six questions. WingBeats, Fall 2022

Stoops, C.A.; Estep, A.S.; Choi, M-Y. Comparison of Mosquito Cuticle Penetration and Feeding Assays to Evaluate Novel Water Soluble Insecticides. US Army Med Dep Journal.

Sen, B.; Gurdal, B.; Estep, A.S.; Tabanca, N.; Kurkcuoglu, M.; Goger, F.; Gul, Z.; Bardakci, H.; Becnel, J.; Mat, A.; & Can Baser, K.H. 2022. The Insecticidal Activities of Erica manipuliflora Extracts in the Flowering and Fruiting Periods and their Evaluation in term of Chemical Profiles of Active Extracts. Industrial Crops & Products. 187(B): 115380. 2022. https://doi.org/10.1016/j.indcrop.2022.115380

Burgess, E.R. IV; Lopez, K.; Irwin, P.; Jaeger, C.P.; & Estep, A.S. 2022. Assessing pyrethroid resistance status in the Culex pipiens complex (Diptera: Culicidae) from the northwest suburbs of Chicago, Illinois using Cox regression of bottle bioassays and other detection tools. PLOS ONE 17(6): e0268205. 2022. https://doi.org/10.1371/journal.pone.0268205

Demiray, H., Estep, A.S., Tabanca, N., Becnel, J.J. and Demirci, B. 2022. Chemical Constituents from Rheum ribes Shoots and its Insecticidal Activity Against Aedes aegypti. Revista Brasileira de Farmacognosia, pp.1-5. doi 10.1007/s43450-021-00224-8

Jensen, B. M., Althoff, R. A., Rydberg, S. E., Royster, E. N., Estep, A., Huijben, S. 2022. Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies. J. Vis. Exp. (179), e63391, doi:10.3791/63391

Kilic, M., Orhan, I. E., Eren, G., Okudan, E. S., Estep, A. S., Bencel, J. J., & Tabanca, N. 2021. Insecticidal activity of forty-seven marine algae species from the Mediterranean, Aegean, and Sea of Marmara in connection with their cholinesterase and tyrosinase inhibitory activity. South African Journal of Botany, 143, 435-442. doi: 10.1016/j.sajb.2021.06.038

Flores-Mendoza, C., Loyola, S., Jiang, J., Farris, C. M., Mullins, K., Estep, A. S., ... & Richards, A. L. 2021. Molecular Characterization of Bartonella Species Discovered in Ectoparasites Collected from Domestic Animals, Cuzco, Peru. Vector-Borne and Zoonotic Diseases, 21(5), 330-341. doi: 10.1089/vbz.2020.2697

Sen-Utsukarci, B., Kessler, S. M., Akbal-Dagistan, O., Estep, A. S., Tabanca, N., Kurkcuoglu, M., ... & Baser, K. H. C. 2021. Chemical composition and biological activities of Valeriana dioscoridis SM. Roots. South African Journal of Botany, 141, 306-312. doi: 10.1016/j.sajb.2021.05.007

Luu-Dam, N. A., Tabanca, N., Estep, A. S., Nguyen, D. H., & Kendra, P. E. 2021. Insecticidal and Attractant Activities of Magnolia citrata Leaf Essential Oil against Two Major Pests from Diptera: Aedes aegypti (Culicidae) and Ceratitis capitata (Tephritidae). Molecules, 26(8), 2311. doi: 10.3390/molecules26082311

Al-Massarani, S.M., El-Gamal, A.A., Al-Rehaily, A.J., Al-Sheddi, E.S., Al-Oqail, M.M., Farshori, N.N., Estep, A.S., Tabanca, N. and Becnel, J.J., 2021. Insecticidal Activity and Free Radical Scavenging Properties of Isolated Phytoconstituents from the Saudi Plant Nuxia oppositifolia (Hochst.). Molecules, 26(4), p.914. doi: 10.3390/molecules26040914

Estep, A.S., Sanscrainte, N.D., Cuba, I., Allen, G.M., Becnel, J.J. and Linthicum, K.J., 2020. Failure of Permethrin-Treated Military Uniforms to Protect Against a Laboratory-Maintained Knockdown-Resistant Strain of Aedes aegypti. Journal of the American Mosquito Control Association, 36(2), pp.127-130. doi: 10.2987/19-6906.1

Mundis, S.J., Estep, A.S., Waits, C.M. and Ryan, S.J., 2020. Spatial variation in the frequency of knockdown resistance genotypes in Florida Aedes aegypti populations. Parasites & Vectors, 13(1), pp.1-12. doi: 10.1186/s13071-020-04112-3

Al-Massarani, S., El-Shaibany, A., Tabanca, N., Ali, A., Estep, A.S., Becnel, J.J., Goger, F., Demirci, B., El-Gamal, A. and Baser, K.H.C., 2019. Assessment of selected Saudi and Yemeni plants for mosquitocidal activities against the yellow fever mosquito Aedes aegypti. Saudi Pharmaceutical Journal, 27(7), pp.930-938. doi: 10.1016/j.jsps.2019.07.001

Tsikolia, M., Bernier, U.R., Agramonte, N.M., Estep, A.S., Becnel, J.J., Linthicum, K.J. and Bloomquist, J.R., 2019. Insecticidal and repellent properties of novel trifluoromethylphenyl amides III. Pesticide biochemistry and physiology, 161, pp.5-11. doi: 10.1016/j.pestbp.2019.06.012

Sever, B., Altıntop, M.D., Özdemir, A., Tabanca, N., Estep, A.S., Becnel, J.J. and Bloomquist, J.R., 2019. Biological evaluation of a series of benzothiazole derivatives as mosquitocidal agents. Open Chemistry, 17(1), pp.288-294. doi: 10.1515/chem-2019-0027

Sen-Utsukarci, B., Taskin, T., Goger, F., Tabanca, N., Estep, A.S., Kessler, S.M., Akbal-Dagistan, O., Bardakci, H., Kurkcuoglu, M., Becnel, J. and Kiemer, A., 2019. Chemical composition and antioxidant, cytotoxic, and insecticidal potential of Valeriana alliariifolia in Turkey. Archives of Industrial Hygiene and Toxicology, 70(3), pp.207-218. doi: 10.2478/aiht-2019-70-3273

Demiray, H., Tabanca, N., Estep, A.S., Becnel, J.J. and Demirci, B., 2019. Chemical composition of the essential oil and n-hexane extract of Stachys tmolea subsp. Tmolea Boiss., an endemic species of Turkey, and their mosquitocidal activity against dengue vector Aedes aegypti. Saudi Pharmaceutical Journal, 27(6), pp.877-881. doi: 10.1016/j.jsps.2019.05.009

Sen-Utsukarci, B., Tabanca, N., Estep, A.S., Akbal-Dağıstan, Ö., Kessler, S.M., Öztürk, Z., Becnel, J., Kiemer, A.K., Mat, A. 2019. The Cytotoxicity and Insecticidal Activity of Extracts from Delphinium formosum Boiss. & Huet. Istanbul J Pharm doi: 10.26650/IstanbulJPharm.2019.19034.

Meepagala, K.M., Estep, A.S., Clausen, B.M. and Becnel, J.J., 2018. Mosquitocidal Activity of a Naturally Occurring Isochroman and Synthetic Analogs from the Plant Pathogenic Fungus, Diaporthe eres Against Aedes aegypti (Diptera: Culicidae). Journal of medical entomology, 55(4), pp.969-974. doi: 10.1093/jme/tjy016

Hajek, A.E., Solter, L.F., Maddox, J.V., Huang, W.F., Estep, A.S., Krawczyk, G., Weber, D.C., Hoelmer, K.A., Sanscrainte, N.D. and Becnel, J.J., 2018. Nosema maddoxi sp. nov.(Microsporidia, Nosematidae), a widespread pathogen of the green stink bug Chinavia hilaris (Say) and the brown marmorated stink bug Halyomorpha halys (Stål). Journal of Eukaryotic Microbiology, 65(3), pp.315-330. doi: 10.1111/jeu.12475

Estep, A.S., Sanscrainte, N.D., Waits, C.M., Bernard, S.J., Lloyd, A.M., Lucas, K.J., Buckner, E.A., Vaidyanathan, R., Morreale, R., Conti, L.A. and Becnel, J.J., 2018. Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse). PLoS neglected tropical diseases, 12(10), p.e0006544. doi: 10.1371/journal.pntd.0006544

Tok, F., Kocyigit‐Kaymakcioglu, B., Tabanca, N., Estep, A.S., Gross, A.D., Geldenhuys, W.J., Becnel, J.J. and Bloomquist, J.R., 2018. Synthesis and structure–activity relationships of carbohydrazides and 1, 3, 4‐oxadiazole derivatives bearing an imidazolidine moiety against the yellow fever and dengue vector, Aedes aegypti. Pest management science, 74(2), pp.413-421. doi: 10.1002/ps.4722

Sanscrainte, N.D., Arimoto, H., Waits, C.M., Li, L.Y., Johnson, D., Geden, C., Becnel, J.J. and Estep, A.S., 2018. Reduction in Musca domestica fecundity by dsRNA-mediated gene knockdown. PloS one, 13(1), p.e0187353. doi: 10.1371/journal.pone.0187353

Sanscrainte, N.D., Waits, C.M., Geden, C.J., Estep, A.S. and Becnel, J.J., 2018. Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies. JoVE (Journal of Visualized Experiments), (141), p.e58650. doi: 10.3791/58650

Shen, Z.H., Sun, Z.H., Becnel, J.J., Estep, A., Wedge, D.E., Tan, C.X., Weng, J.Q., Han, L. and Liu, X.H., 2018. Synthesis and Mosquiticidal Activity of Novel Hydrazone Containing Pyrimidine Derivatives against Aedes aegypti. Letters in Drug Design & Discovery, 15(9), pp.951-956. doi: 10.2174/1570180815666180102141640

Dunford, J.C., Estep, A.S., Waits, C.M. et al. 2018. Evaluation of the long-term efficacy of K-Othrine® PolyZone on three surfaces against laboratory reared Anopheles gambiae in semi-field conditions. Malar J 17, 94. doi: 10.1186/s12936-018-2239-z

Tsikolia, M., Bernier, U.R., Agramonte, N.M., Estep, A.S., Becnel, J.J., Tabanca, N., Linthicum, K.J., Gross, A.D., Guerin, P.M., Kröber, T. and Bloomquist, J.R., 2018. Insecticidal and repellent properties of novel trifluoromethylphenyl amides II. Pesticide biochemistry and physiology, 151, pp.40-46. doi: 10.1016/j.pestbp.2018.08.006

Liu, X.H., Wang, Q., Sun, Z.H., Wedge, D.E., Becnel, J.J., Estep, A.S., Tan, C.X. and Weng, J.Q., 2017. Synthesis and insecticidal activity of novel pyrimidine derivatives containing urea pharmacophore against Aedes aegypti. Pest management science, 73(5), pp.953-959. doi: 10.1002/ps.4370

Gundersen-Rindal, D., Adrianos, S., Allen, M.M., Becnel, J., Chen, Y.P., Choi, M.Y., Estep, A., Evans, J.D., Garczynski, S. and Geib, S., 2017. Arthropod genomics research in the United States Department of Agriculture-Agricultural Research Service: applications of RNA interference and CRISPR gene editing technologies in pest control. Trends Entomol., 13, pp.109-137. http://www.researchtrends.net/tia/abstract.asp?in=0&vn=13&tid=20&aid=6045&pub=2017&type=3

Estep, A.S., Sanscrainte, N.D., Waits, C.M., Louton, J.E. and Becnel, J.J., 2017. Resistance status and resistance mechanisms in a strain of Aedes aegypti (Diptera: Culicidae) from Puerto Rico. Journal of medical entomology, 54(6), pp.1643-1648. doi: 10.1093/jme/tjx143

Waits, C.M., Fulcher, A., Louton, J.E., Richardson, A.G., Becnel, J.J. and Estep, A.S., 2017. A comparative analysis of resistance testing methods in Aedes albopictus (Diptera: Culicidae) from St. Johns County, Florida. Florida Entomologist, 100(3), pp.571-578. doi: 10.1653/024.100.0313

Lloyd, A.M., Farooq, M., Estep, A.S., Xue, R.D. and Kline, D.L., 2017. Evaluation of pyriproxyfen dissemination via Aedes albopictus from a point-source larvicide application in northeast Florida. Journal of the American Mosquito Control Association, 33(2), pp.151-155. doi: 10.2987/14-6459.1

Demiray, H., Tabanca, N., Goger, F., Estep, A.S., Becnel, J.J. and Demirci, B., 2017. Chemical Composition and Mosquitocidal Activity of n-Hexane and Methanolic Extracts from Euphorbia anacampseros var. tmolea: An Endemic Species of Turkey against Aedes aegypti. Asian Journal of Chemistry, 29(11), pp.2488-2492. doi: 10.14233/ajchem.2017.20813

Estep, A.S., Sanscrainte, N.D. and Becnel, J.J., 2016. DsRNA-mediated targeting of ribosomal transcripts RPS6 and RPL26 induces long-lasting and significant reductions in fecundity of the vector Aedes aegypti. Journal of insect physiology, 90, pp.17-26. doi: 10.1016/j.jinsphys.2016.05.001

Meepagala, K.M., Estep, A.S. and Becnel, J.J., 2016. Larvicidal and Adulticidal Activity of Chroman and Chromene Analogues against Susceptible and Permethrin-Resistant Mosquito Strains. Journal of agricultural and food chemistry, 64(24), pp.4914-4920. doi: 10.1021/acs.jafc.6b01299

Weng, J.Q., Ali, A., Estep, A., Becnel, J., Meyer, S.L., Wedge, D.E., Jacob, M. and Rimando, A.M., 2016. Synthesis and Biological Evaluation of 3, 5‐Dimethoxystilbene Analogs. Chemistry & biodiversity, 13(9), pp.1165-1177. doi: 10.1002/cbdv.201500516

Estep, A.S., Becnel, J.J. and Lee, S.T., 2016. Toxicity of Compounds Isolated from White Snakeroot (Ageratina altissima) to Adult and Larval Yellow Fever Mosquitoes (Aedes aegypti). Natural product communications, 11(11), pp.1675-1677. doi: 10.1177/1934578X1601101113

Al-Massarani, S., Al-Enzi, F., Al-Tamimi, M., Al-Jomaiah, N., Al-amri, R., Başer, K.H.C., Tabanca, N., Estep, A.S., Becnel, J.J., Bloomquist, J.R. and Demirci, B., 2016. Composition & biological activity of Cyperus rotundus L. tuber volatiles from Saudi Arabia. Natural Volatiles & Essential Oils, 3(2), pp.26-34. https://dergipark.org.tr/en/pub/nveo/issue/24570/260181

Meepagala, K.M., Estep, A.S. and Becnel, J.J., 2016. Mosquitocidal Activity of Extracts from Ammi visnaga (Apiaceae) Seeds. Journal of Agricultural Chemistry and Environment, 5(04), p.170. doi: 10.4236/jacen.2016.54018

Hoy, M.A., Waterhouse, R.M., Wu, K., Estep, A.S., Ioannidis, P., Palmer, W.J., Pomerantz, A.F., Simao, F.A., Thomas, J., Jiggins, F.M. and Murphy, T.D., 2016. Genome sequencing of the phytoseiid predatory mite Metaseiulus occidentalis reveals completely atomized Hox genes and superdynamic intron evolution. Genome biology and evolution, 8(6), pp.1762-1775. doi: 10.1093/gbe/evw048

Puglise, J.M., Estep, A.S. and Becnel, J.J., 2016. Expression Profiles and RNAi Silencing of Inhibitor of Apoptosis Transcripts in Aedes, Anopheles, and Culex Mosquitoes (Diptera: Culicidae). Journal of medical entomology, 53(2), 304-314. doi: 10.1093/jme/tjv191

Meepagala, K.M., Becnel, J.J. and Estep, A.S., 2015. Phomalactone as the Active Constituent against Mosquitoes from Nigrospora spherica. Agricultural Sciences, 6(10), p.1195. doi: 10.4236/as.2015.610114

Choi, M.Y., Sanscrainte, N.D., Estep, A.S., Vander Meer, R.K. and Becnel, J.J., 2015. Identification and expression of a new member of the pyrokinin/pban gene family in the sand fly Phlebotomus papatasi. Journal of insect physiology, 79, pp.55-62. doi: 10.1016/j.jinsphys.2015.06.001

Sarikahya, N.B., Kayce, P., Tabanca, N., Estep, A.S., Becnel, J.J., Khan, I.A. and Kirmizigul, S., 2015. Toxicity of Cephalaria Species and their Individual Constituents against Aedes aegypti. Natural product communications, 10(7), pp.1195-1198. doi: 10.1177/1934578X1501000715

Plischuk, S., Sanscrainte, N.D., Becnel, J.J., Estep, A.S. and Lange, C.E., 2015. Tubulinosema pampeana sp. n. (Microsporidia, Tubulinosematidae), a pathogen of the South American bumble bee Bombus atratus. Journal of invertebrate pathology, 126, pp.31-42. doi: 10.1016/j.jip.2015.01.006

Wanner, J., Tabanca, N., Zehl, M., Jirovetz, L., Schmidt, E., Patschka, A., Ali, A., S Estep, A., J Becnel, J., Y Li, A. and A Khan, I., 2015. Investigations into the chemistry and insecticidal activity of Euonymus europaeus seed oil and methanol extract. Current Bioactive Compounds, 11(1), pp.13-22. https://www.ingentaconnect.com/content/ben/cbc/2015/00000011/00000001/art00005#expand/collapse

Dunford, J.C., Stoops, C.A., Estep, A.S., Britch, S.C., Richardson, A.G., Walker, T.W., Farooq, M., Hoel, D.F., Platt, R.R., Smith, V.L. and Wirtz, R.A., 2014. SR450 And Superhawk XP Applications of Bacillus thuringiensis israelensis against Culex quinquefasciatus. Journal of the American Mosquito Control Association, 30(3), pp.191-198. doi: 10.2987/14-6400R.1

Chang, F., Dutta, S., Becnel, J. J., Estep, A. S., & Mascal, M. 2014. Synthesis of the insecticide prothrin and its analogues from biomass-derived 5-(chloromethyl) furfural. Journal of agricultural and food chemistry, 62(2), 476-480. doi: 10.1021/jf4045843

Reid, W.R., Thornton, A., Pridgeon, J.W., Becnel, J.J., Tang, F., Estep, A., Clark, G.G., Allan, S. and Liu, N., 2014. Transcriptional analysis of four family 4 P450s in a Puerto Rico strain of Aedes aegypti (Diptera: Culicidae) compared with an Orlando strain and their possible functional roles in permethrin resistance. Journal of medical entomology, 51(3), pp.605-615. doi: 10.1603/ME13228

Akdag, K., Kocyigit-Kaymakcioglu, B., Tabanca, N., Ali, A., Estep, A., Becnel, J. J., & Khan, I. A. 2014. Synthesis and larvicidal and adult topical activity of some hydrazide-hydrazone derivatives against Aedes aegypti, Marmara Pharm J.9, 10. doi: 10.12991/mpj.2014187238

Choi, M.Y., Estep, A., Sanscrainte, N., Becnel, J. and Vander Meer, R.K., 2013. Identification and expression of PBAN/diapause hormone and GPCRs from Aedes aegypti. Molecular and cellular endocrinology, 375(1-2), pp.113-120. doi: 10.1016/j.mce.2013.05.019

Lloyd AM, Farooq M, Diclaro JW, Kline DL, Estep AS. 2013. Field Evaluation of Commercial Off-the-Shelf Spatial Repellents Against the Asian Tiger Mosquito, Aedes Albopictus (Skuse), and the Potential for Use During Deployment. US Army Med Dep J. 2013 Apr-Jun:80-6. https://pubmed.ncbi.nlm.nih.gov/23584913/

Farooq, M., Lloyd, A.M., Estep, A.S., Walker, T.W. and Hughes, T., 2013. Temporal and Storage Effects on Ultra-Low Volume Droplets of Insecticides Collected on Teflon®-Coated Slides1. Journal of the American Mosquito Control Association, 29(4), pp.389-392. doi: 10.2987/13-6333.1

Estep AS, Zettel-Nalen C, Ellis JD (2013, February) Blue Orchard Bee (Osmia lignaria Say). UF/IFAS Featured Creatures. Retrieved May 27, 2013 from http://entnemdept.ufl.edu/creatures/MISC/BEES/blue_orchard_bee.htm

Koski CL, Estep AS, Sawant-Mane S, Shin ML, Highbarger L, & Hansch GM (1996). Complement regulatory molecules on human myelin and glial cells: differential expression affects the deposition of activated complement proteins. Journal of neurochemistry, 66(1), 303-312. doi: 10.1046/j.1471-4159.1996.66010303.x

Sawant-Mane S, Estep III A, & Koski CL (1994). Antibody of patients with Guillain-Barré syndrome mediates complement-dependent cytolysis of rat Schwann cells: susceptibility to cytolysis reflects Schwann cell phenotype. Journal of neuroimmunology, 49(1), 145-152. doi: 10.1016/0165-5728(94)90190-2

 

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CONFERENCE PRESENTATIONS AND SEMINARS

2024    Invited Presentation: “Molecular Surveillance of Insecticide Resistance Markers and Testing Results from Haiti” GEIS Vector Surveillance Summit, Silver Spring, MD September 5, 2024.

2024    Invited Presentation: “Collaboration as a tool to effectively assess insecticide resistance in mosquitoes and implement improved operational responses” CDC Arboviral Diseases Branch, Ft. Collins, CO August 12, 2024. (declined)

2024    Invited Presentation: “The current state of insecticide resistance in Florida mosquitoes” Florida Department of Agriculture and Consumer Services CEU Webinar Series, Virtual, May 14, 2024.

2024    Invited Presentation:   “Three vectors, Three Versions of Insecticide Resistance and Three Strategies for Effective Management” Florida Annual Entomologist/Biologist Workshop, Indian River, FL, May 07, 2024.

2024    Invited Presentation: “Rapid insecticide resistance testing during an emergency response” CDC/NACCHO 2024 Vector Control Hurricane Crisis Workshop St Augustine, FL April 22-25, 2024.

2024    Presentation: “Monitoring and managing insecticide resistance” American Mosquito Control Association 90th Annual Meeting, Dallas, TX March 4-8, 2024.

2024    Invited Seminar: “Dodd 2024- The History of Insecticide Resistance” 40^th Annual Dodd Short Courses, Gainesville, FL January 30, 2024.

2024    Invited Presentation: “Next Steps: Characterizing Insecticide Resistance after the CDC Bottle Bioassay” 40^th Annual Dodd Short Courses, Gainesville, FL January 31, 2024.

2023    Invited Presentation: “State of the State: An update on insecticide resistance in Florida” Florida Coordinating Council on Mosquito Control Fall Meeting, Lecanto, FL October 24, 2023.

2023    Invited Training Webinar: “AMCA's Best Practices for Integrated Mosquito Management Virtual Training Program- Insecticide Resistance - Module 12” American Mosquito Control Association, October, 2023.

2023    Invited Presentation: “The Integrated Vector Management approach to effective resistance management” American Mosquito Control Association Annual Meeting, Reno, NV, March 01, 2023.

2023    Invited Presentation: “Mechanism matters: Efficacy of commercial formulations and the relationship to underlying insecticide resistance mechanisms” American Mosquito Control Association Annual Meeting, Reno, NV, March 01, 2023. (presented by Neil Sanscrainte)

2023    Invited Presentation: “Mapping kdr genotypes in Arizona populations of Aedes aegypti” American Mosquito Control Association Annual Meeting, Reno, NV, March 01, 2023.

2022    Invited Presentation: “Relating laboratory insecticide resistance testing to operational efficacy in mosquitoes.” American Chemical Society Annual Meeting, Chicago, IL, August 21-25, 2022.

2022    Invited Webinar Speaker: “Getting real about resistance: Relating laboratory assays to field interventions.” American Mosquito Control Association, May 11, 2022.

2022    Invited Virtual Presentation: “Laboratory and semi-field evaluation of insecticide resistance in field collected Culex quinquefasciatus.” 17^th Annual Arbovirus Surveillance and Mosquito Control Workshop, St. Augustine, FL, March 29, 2022. (presented by Neil Sanscrainte)

2022    Invited Virtual Presentation: “Getting real with resistance: Applying lab testing results to field operations.” 17^th Annual Arbovirus Surveillance and Mosquito Control Workshop, St. Augustine, FL, March 29, 2022.

2021    Invited Virtual Presentation: “Generating resistance information from arthropod samples destined for pathogen testing: increasing operational value for effective pest control.” Global Emerging Infections Surveillance- Armed Forces Health Surveillance Branch Teleconference, March 23, 2021.

2021    Virtual Presentation: “In vivo laboratory and operational quantification of kdr mutations in Aedes aegypti.” American Mosquito Control Association Annual Meeting, March 03, 2021.

2021    Virtual Presentation: “Genetic isolation and toxicological evaluation of the L1014F kdr mutation in field collected Culex quinquefasciatus from Louisiana.“ 87^th American Mosquito Control Association Annual Meeting, March 02, 2021. (presented by Neil Sanscrainte)

2021    Invited Virtual Presentation: “Insecticide Resistance in FL: What we know, how we use it, and what we still need to do.” FL Coordinating Council for Mosquito Control, January 19, 2021.

2020    Invited Virtual Presentation: “Quantification of the effects of Aedes aegypti kdr mutations and an update on findings in AZ.” 2020 Arizona Virtual Arbovirus Discussion Group, December 11, 2020.

2020    Invited Virtual Presentation: “Laboratory and operational quantification of kdr mutations in Aedes aegypti.” Louisiana Mosquito Control Association Annual Meeting, December 08, 2020.

2020    Invited Virtual Presentation: “Genetic isolation and toxicological evaluation of the L1014F kdr mutation in field collected Culex quinquefasciatus from Louisiana. “ Louisiana Mosquito Control Association Annual Meeting, December 08, 2020. (presented by Neil Sanscrainte)

2020    Presentation: “Using MinION: Changing the way we sequence.” Joint Base Pearl Harbor, Honolulu, HI, February 2020.