| Kable Lab |
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KABLE LAB
Mary Kable, Ph.D. Research Scientist Adjunct Assistant Professor, UC Davis Department of Nutrition Office: (530) 752-1607 Email address: mary.kable@usda.gov Research Interests The Kable laboratory is interested in mechanisms governing how diet impacts the bacterial composition of the human gut and how these diet-bacterial interactions can influence human health. In particular, we are interested in how dietary fiber can affect the composition of the gut microbiota in such a way as to increase or decrease colonization resistance and susceptibility to food borne pathogens. Kable Lab Themes The Kable Lab research is currently focused on (1) characterization of the gut microbiota in a healthy human cohort and potential interactions between this composition and the long term dietary habits of healthy individuals, (2) optimization of methods for medium through-put culture of diverse gut microbiota, (3) in vitro fermentation of complete gut microbiota and individual bacteria from healthy human subjects with various food components, especially dietary fiber.
ARS Employees David Storms, Support Scientist Laurynne Coates, USDA Postdoctoral Researcher Trainees Ste Traxler, Graduate Student Researcher Sarah Spearman, UC Davis Junior Specialist Alyce Faso, UC Davis Undergraduate Volunteer
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Riley, H.L., Kable, M.E., Marco, M.L., Keim, N.L. 2019. The role of the gut microbiome in predicting response to diet and the development of precision nutrition models. Part II: Results. Advances in Nutrition. https://doi.org/10.1093/advances/nmz049.
Westreich, S.T., Ardeshir, A., Alkan, Z., Kable, M.E., Korf, I., Lemay, D.G. 2019. Fecal metatranscriptomics of macaques with idiopathic chronic diarrhea reveals altered mucin degradation and fucose utilization. BMC Microbiome. 7:41. https://doi.org/10.1186/s40168-019-0664-z.
Hughes, R.L., Marco, M., Hughes, J.P., Keim, N.L., Kable, M.E. 2019. The role of the gut microbiome in predicting response to diet and the development of precision nutrition models. Part I: Overview of Current Methods. Advances in Nutrition. https://doi.org/10.1093/advances/nmz022.
Kable, M.E., Hansen, L.M., Styer, C.M., Gideonsson, P., Deck, S.L., Shevtsova, A., Rakhimova, O., Eaton, K.A., Martin, M.E., Boren, T., Solnick, J.V. 2017. Host determinants of expression of the helicobacter pylori BabA adhesin. Scientific Reports. 7:46499.
Cooper, D.N., Kable, M.E., Marco, M.L., De Leon, A., Rust, B.M., Baker, J.E., Horn, W.F., Burnett, D., Keim, N.L. 2017. The effects of moderate whole grain consumption on fasting glucose and lipids, gastrointestinal symptoms, and microbiota. Nutrients. 9(2):173. doi: 10.3390/nu9020173.
Westreich, S.T., Ardeshir, A., Kable, M.E., Korf, I., Lemay, D.G. 2016. The metatranscriptome of the rhesus macaque: investigating potential causes of idiopathic chronic diarrhea. Experimental Biology. 31/650.
Impact of Diet on Intestinal Microbiota, Gut Health and Immune Function In-House Appropriated (D) Accession Number:432895 Assessment of Antimicrobial Resistance Genes in Human Fecal Samples Non-Assistance Cooperative Agreement (S) Accession Number:436885 Mothers, Infants and Lactation Quality-Extra Analyses (MILQ II) Project II Trust Fund Cooperative Agreement (T) Accession Number:437095 The Role of Honey and Small Intestine Microbes in Digestive Health Trust Fund Cooperative Agreement (T) Accession Number:435767 Association of Dairy Intake With Protection Against Gastrointestinal Inflammation Trust Fund Cooperative Agreement (T) Accession Number:436064
Methods and Technique The Kable Lab focuses on methods for the analysis of human gut microbes. For growth and fermentation experiments we use a: 2.7’ x 6.5’ Coy vinyl anaerobic chamber with space for two people to work, equipped with • airlock, •oxygen/hydrogen monitor, • hydrogen sulfide removal system, • incubator • and SPECTROstar Nano full UV/Vis spectrum absorbance microplate reader with cuvette port Endpoints analyzed in the Kable Lab: • 16S rRNA sequence based assessment of human gut microbial communities • Cultured bacterial isolates from human gut samples • Bacterial growth curves, antibiotic susceptibility, etc. • Trans-epithelial resistance of colonic epithelial cells after exposure to microbial fermentation products
