Skip to main content
ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics and Animal Breeding » People & Locations » Gregory Harhay
Gregory P Harhay
Computational Biologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Escherichia coli O157:H7 tir 255 T > A allele strains differ in chromosomal and plasmid composition - (Peer Reviewed Journal)
Weinroth, M.D., Clawson, M.L., Harhay, G.P., Eppinger, M., Harhay, D.M., Smith, T.P.L., Bono, J.L. 2023. Escherichia coli O157:H7 tir 255 T > A allele strains differ in chromosomal and plasmid composition. Frontiers in Microbiology. 14. Article 1303387. https://doi.org/10.3389/fmicb.2023.1303387.
Two bovine hepacivirus genome sequences from U.S. cattle - (Peer Reviewed Journal)
Workman, A.M., Harhay, G.P., Groves, J.T., Vander Ley, B.L. 2024. Two bovine hepacivirus genome sequences from U.S. cattle. Journal of Veterinary Diagnostic Investigation. 36(2):274-277. https://doi.org/10.1177/10406387231225656.
First gene-edited calf with reduced susceptibility to a major viral pathogen - (Peer Reviewed Journal)
Workman, A.M., Heaton, M.P., Vander Ley, B., Webster, D., Sherry, L., Bostrom, J.R., Larson, S., Kalbfleisch, T., Harhay, G.P., Jobman, E., Carlson, D., Sonstegard, T.S. 2023. First gene-edited calf with reduced susceptibility to a major viral pathogen. Proceedings of the National Academy of Sciences-Nexus. 2(5). Article pgad125. https://doi.org/10.1093/pnasnexus/pgad125.
A CD46-edited calf with reduced susceptibility to bovine viral diarrhea virus - (Abstract Only)
First gene-edited calf with reduced susceptibility to a major viral pathogen - ()
Workman, A.M., Heaton, M.P., Vander Ley, B.L., Webster, D.A., Sherry, L., Larson, S., Kalbfleisch, T.S., Harhay, G.P., Jobman, E.E., Carlson, D.F., Sonstegard, T.S. 2022. First gene-edited calf with reduced susceptibility to a major viral pathogen. bioRxiv. Article 519336. https://doi.org/10.1101/2022.12.08.519336.
Recent emergence of bovine coronavirus variants with mutations in the hemagglutinin-esterase receptor binding domain in U.S. cattle - (Peer Reviewed Journal)
Workman, A.M., McDaneld, T.G., Harhay, G.P., Das, S., Loy, J.D., Hause, B.M. 2022. Recent emergence of bovine coronavirus variants with mutations in the hemagglutinin-esterase receptor binding domain in U.S. cattle. Viruses. 14(10). Article 2125. https://doi.org/10.3390/v14102125.
Table 1. Genome sequence context for JMJD1C SNPs associated with bovine congestive heart failure (BCHF) - ()
Heaton, M., Harhay, G., Smith, T.P.L., Kuhn, K., Kalbfleisch, T.S., Jobman, E., Vander Ley, B. 2022. Table 1. Genome sequence context for JMJD1C SNPs associated with bovine congestive heart failure (BCHF). Figshare. Dataset. https://doi.org/10.6084/m9.figshare.19780558.v1.
Discrete distributions of simple sequence repeat lengths in DNA template vary at individual loci in Mannheimia haemolytica isolates - (Proceedings)
Association of ARRDC3 and NFIA variants with bovine congestive heart failure in feedlot cattle - (Peer Reviewed Journal)
Heaton, M.P., Harhay, G.P., Bassett, A.S., Clark, H.J., Carlson, J.M., Jobman, E.E., Sadd, H.R., Pelster, M.C., Workman, A.M., Kuehn, L.A., Kalbfleisch, T.S., Piscatelli, H., Carrie, M., Krafsur, G.M., Grotelueschen, D.M., Vander Ley, B.L. 2022. Association of ARRDC3 and NFIA variants with bovine congestive heart failure in feedlot cattle. F1000Research. 11. Article 385. https://doi.org/10.12688/f1000research.109488.1 .
Evaluating large spontaneous deletions in a bovine cell line selected for bovine viral diarrhea virus resistance - (Peer Reviewed Journal)
Workman, A.M., Heaton, M.P., Webster, D.A., Harhay, G.P., Kalbfleisch, T.S., Smith, T.P.L., Falkenberg, S.M., Carlson, D.F., Sonstegard, T.S. 2021. Evaluating large spontaneous deletions in a bovine cell line selected for bovine viral diarrhea virus resistance. Viruses. 13(11). Article 2147. https://doi.org/10.3390/v13112147.
A conserved Histophilus somni 23S intervening sequence yields functional, fragmented 23S rRNA - (Peer Reviewed Journal)
Harhay, G.P., Harhay, D.M., Brader, K.D., Smith, T.P.L. 2021. A conserved Histophilus somni 23S intervening sequence yields functional, fragmented 23S rRNA. Microbiology Spectrum. 9(3). Article e0143121. https://doi.org/10.1128/Spectrum.01431-21.
Detection of bovine inflammatory cytokines IL-1ß, IL-6, and TNF-a with a multiplex electrochemiluminescent assay platform Reprint Icon - (Peer Reviewed Journal)
Chitko-McKown, C.G., Bierman, S.L., Kuehn, L.A., Bennett, G.L., DeDonder, K.D., Apley, M.D., Harhay, G.P., Clawson, M.L., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V. 2021. Detection of bovine inflammatory cytokines IL-1ß, IL-6, and TNF-a with a multiplex electrochemiluminescent assay platform. Veterinary Immunology and Immunopathology. 237. Article 110274. https://doi.org/10.1016/j.vetimm.2021.110274.
Cytokine and haptoglobin profiles from shipping through sickness and recovery in metaphylaxis- or un-treated cattle - (Peer Reviewed Journal)
Chitko-McKown, C.G., Bennett, G.L., Kuehn, L.A., DeDonder, K.D., Apley, M.D., Harhay, G.P., Clawson, M.L., Workman, A.M., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V. 2021. Cytokine and haptoglobin profiles from shipping through sickness and recovery in metaphylaxis- or un-treated cattle. Frontiers in Veterinary Science. 8. Article 611927. https://doi.org/10.3389/fvets.2021.611927.
Rapid estimation of Salmonella enterica contamination level in ground beef – application of the time-to-positivity method using a combination of molecular detection and direct plating Reprint Icon - (Peer Reviewed Journal)
Harhay, D.M., Weinroth, M.D., Bono, J.L., Harhay, G.P., Bosilevac, J.M. 2021. Rapid estimation of Salmonella enterica contamination level in ground beef – application of the time-to-positivity method using a combination of molecular detection and direct plating. Food Microbiology. 93. Article 103615. https://doi.org/10.1016/j.fm.2020.103615.
Classification of 16S rRNA reads is improved using a niche-specific database constructed by near-full length sequencing - (Peer Reviewed Journal)
Myer, P.R., McDaneld, T.G., Kuehn, L.A., Dedonder, K.D., Apley, M.D., Capik, S.F., Lubbers, B.V., Harhay, G.P., Harhay, D.M., Keele, J.W., Henniger, M.T., Clemmons, B.A., Smith, T.P.L. 2020. Classification of 16S rRNA reads is improved using a niche-specific database constructed by near-full length sequencing. PLoS One. 15(7):e0235498. https://doi.org/10.1371/journal.pone.0235498.
Association of ARRDC3 and NF1A genes with bovine congestive heart failure - (Abstract Only)
Heaton, M.P., Harhay, G.P., Bassett, A.S., Clark, H.J., Carlson, J.M., Smith, H.R., Pelster, M.C., Workman, A.M., Grotelueschen, D.M., Vander Ley, B.L. 2020. Association of ARRDC3 and NF1A genes with bovine congestive heart failure. In: Plant and Animal Genome Conference Proceedings, January 11-15, 2020, San Diego, California. PE0326.
A computational method to quantify the effects of slipped strand mispairing on bacterial tetranucleotide repeats - (Peer Reviewed Journal)
Harhay, G.P., Harhay, D.M., Bono, J.L., Capik, S.F., DeDonder, K.D., Apley, M.D., Lubbers, B.V., White, B.J., Larson, R.L., Smith, T.P.L. 2019. A computational method to quantify the effects of slipped strand mispairing on bacterial tetranucleotide repeats. Nature Scientific Reports. 9:18087. https://doi.org/10.1038/s41598-019-53866-z.
Complete genome sequence of a Salmonella enterica subsp. enterica serovar Fresno isolate recovered from beef cattle lymph nodes - (Peer Reviewed Journal)
Haley, B.J., Smith, T.P., Harhay, G.P., Loneragan, G.H., Webb, H.E., Bugarel, M., Kim, S., Van Kessel, J.S., Harhay, D.M. 2019. Complete genome sequence of a Salmonella enterica subsp. enterica serovar Fresno isolate recovered from beef cattle lymph nodes. Microbiology Resource Announcements. 8(2):e01338-18. https://doi.org/10.1128/MRA.01338-18.
Complete closed genome sequences of three Salmonella enterica subsp. enterica Serovar Dublin strains isolated from cattle at harvest - (Peer Reviewed Journal)
Harhay, D.M., Smith, T.P.L., Harhay, G.P., Loneragan, G.H., Webb, H.E., Bugarel, M., Haley, B.J., Kim, S.W., Van Kessel, J.S. 2018. Complete closed genome sequences of three Salmonella enterica subsp. enterica Serovar Dublin strains isolated from cattle at harvest. Microbiology Resource Announcements. 7:e01334-18. https://doi.org/10.1128/MRA.01334-18.
Closed genome sequences and antibiograms of 16 pasteurella multocida isolates from bovine respiratory disease complex cases and apparently healthy controls - (Peer Reviewed Journal)
Harhay, G.P., Harhay, D.M., Bono, J.L., Smith, T.P.L., Capik, S.F., DeDonder, K.D., Apley, M.D., Lubbers, B.V., White, B.J., Larson, R.L. 2018. Closed genome sequences and antibiograms of 16 pasteurella multocida isolates from bovine respiratory disease complex cases and apparently healthy controls. Microbiology Resource Announcements. 7(11):e00976-18. https://doi.org/10.1128/MRA.00976-18.
Comparative genomics of Salmonella enterica serovar Montevideo reveals lineage-specific gene differences that may influence ecological niche association - (Peer Reviewed Journal)
Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovina, M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2018. Comparative genomics of Salmonella enterica serovar Montevideo reveals lineage-specific gene differences that may influence ecological niche association. Microbial Genomics. 4:1-17. https://doi.org/10.1099/mgen.0.000202.
Transcriptome responses to respiratory virus infection of pigs within the tracheobronchial lymphnode following infection with PRRSV, PCV2 or IAV - (Abstract Only)
Miller, L.C., Fleming, D.S., Harhay, G.P., Kehrli, Jr., M.E., Lager, K.M. 2018. Transcriptome responses to respiratory virus infection of pigs within the tracheobronchial lymphnode following infection with PRRSV, PCV2 or IAV. The Journal of Immunology. Vol. 200(1 Supplement):59.14.
Transcriptome responses to respiratory virus infection of pigs within the tracheobronchial lymphnode following infection with PRRSV, PCV2 or IAV - (Abstract Only)
Miller, L.C., Fleming, D.S., Harhay, G.P., Kehrli, Jr., M.E., Lager, K.M. 2018. Transcriptome responses to respiratory virus infection of pigs within the tracheobronchial lymphnode following infection with PRRSV, PCV2 or IAV. Plant & Animal Genome Conference XXVI, January 13-17, 2018, San Diego, California. Paper No. P1222.
Comparison of the transcriptome response within the tracheobronchial lymphnode following infection with PRRSV, PCV2 or SIV - (Abstract Only)
Miller, L.C., Fleming, D., Harhay, G., Bayles, D., Kehrli, M., Lager, K. 2017. Comparison of the transcriptome response within the tracheobronchial lymphnode following infection with PRRSV, PCV2, or SIV. 98th Conference of Research Workers in Animal Diseases. Abstract No. 190, p. 107.
Closed genome sequences of seven histophilus somni isolates from beef calves with bovine respiratory disease complex Reprint Icon - (Peer Reviewed Journal)
Harhay, G.P., Harhay, D.M., Bono, J.L., Smith, T.P.L., Capik, S.F., DeDonder, K.D., Apley, M.D., Lubbers, B.V., White, B.J., Larson, R.L. 2017. Closed genome sequences of seven histophilus somni isolates from beef calves with bovine respiratory disease complex. Genome Announcements. 5(40):e01099-17. https://doi.org/10.1128/genomeA.01099-17.
Whole genome sequencing may not be adequate to determine genome relatedness for surveillance and outbreak investigation of foodborne pathogens - (Abstract Only)
Bono, J.L., Arthur, T.M., Harhay, G.P., Smith, T.P. 2017. Whole genome sequencing may not be adequate to determine genome relatedness for surveillance and outbreak investigation of foodborne pathogens. [Abstract]. 12th Annual Sequencing, Finish, and Analysis in the Future Meeting, PS-2.32. Available: http://www.lanl.gov/conferences/sequencing-finishing-analysis-future/index.php.
Complete genome sequence of the thermotolerant foodborne pathogen Salmonella enterica serovar Senftenberg ATCC 43845 and phylogenetic analysis of loci encoding thermotolerance - (Abstract Only)
Nguyen, S.V., Bono, J.L., Smith, T.P., Harhay, G.P., Harhay, D.M. 2017. Complete genome sequence of the thermotolerant foodborne pathogen Salmonella enterica Serovar Senftenberg ATCC 43845 and phylogenetic analysis of Loci encoding thermotolerance . [Abstract] International Association for Food Protection Proceedings. Session T2-09. Available at: https://iafp.confex.com/iafp/2017/webprogram/authork.html.
Closed genomes and phenotypes of seven Histophilus somni isolates from beef calves with bovine respiratory disease complex - (Abstract Only)
Harhay, G.P., Harhay, D.M., Bono, J.L., Smith, T.P., Heaton, M.P., Clawson, M.L., Chitko-McKown, C.G., Capik, S.F., DeDonder, K.D., Apley, M.D., Lubbers, B.V., White, B.J., Larson, R.L. 2017. Closed genomes and phenotypes of seven Histophilus somni isolates from beef calves with bovine respiratory disease complex [Abstract]. American Society for Microbiology Microbe Meeting, June 1-5, 2017, New Orleans, LA. Poster #Sunday-869.
Genome sequence of the thermotolerant foodborne pathogen Salmonella enterica serovar Senftenberg ATCC 43845 and phylogenetic analysis of Loci encoding increased protein quality control mechanisms - (Peer Reviewed Journal)
Nguyen, S.V., Harhay, G.P., Bono, J.L., Smith, T.P., Harhay, D.M. 2017. Genome sequence of the thermotolerant foodborne pathogen Salmonella enterica serovar Senftenberg ATCC 43845 and phylogenetic analysis of Loci encoding increased protein quality control mechanisms. mSystems. 2:e00190-16. https://doi.org/10.1128/mSystems.00190-16.
Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes - (Peer Reviewed Journal)
Clawson, M.L., Murray, R.W., Sweeney, M.T., Apley, M.D., DeDonder, K.D., Capik, S.F., Larson, R.L., Lubbers, B.V., White, B.J., Kalbfleisch, T.S., Schuller, G., Dickey, A.M., Harhay, G.P., Heaton, M.P., Chitko-McKown, C.G., Brichta-Harhay, D.M., Bono, J.L., Smith, T.P.L. 2016. Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes. BMC Genomics. 17:982. doi: 10.1186/s12864-016-3316-8.
Haptoglobin concentrations in cattle either mass-medicated with gamithromycin or sham-treated - from shipping through sickness and recovery - (Abstract Only)
Chitko-McKown, C.G., Workman, A.M., DeDonder, K.D., Bennett, G.L., Apley, M.E., Harhay, G.P., Harhay, D.M., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Clawson, M.L. 2016. Haptoglobin concentrations in cattle either mass-medicated with gamithromycin or sham-treated - from shipping through sickness and recovery [Abstract]. Conference of Research Workers in Animal Diseases, December 4-6, 2016, Chicago, IL. Abstract #135.
Complete, closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Typhimurium strains isolated from human and bovine sources Reprint Icon - (Peer Reviewed Journal)
Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2016. Complete, closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Typhimurium strains isolated from human and bovine sources. Genome Announcements. 4(6):e01212-16. doi:10.1128/genomeA.01212-16.
Comparative methylome analysis of the occasional ruminant respiratory pathogen Bibersteinia trehalosi - (Peer Reviewed Journal)
Anton, B.P., Harhay, G.P., Smith, T.P.L., Blom, J., Roberts, R.J. 2016. Comparative methylome analysis of the occasional ruminant respiratory pathogen Bibersteinia trehalosi. PLoS One. 11(8): e0161499.
Observations on macrolide resistance and susceptibility testing performance in field isolates collected from clinical bovine respiratory disease cases - (Peer Reviewed Journal)
DeDonder, K.D., Harhay, D.M., Apley, M.D., Lubbers, B.V., Clawson, M.L., Schuller, G., Harhay, G.P., White, B.J., Larson, R.L., Capik, S.F., Riviere, J.E., Kalbfleisch, T., Tessman, R.K. 2016. Observations on macrolide resistance and susceptibility testing performance in field isolates collected from clinical bovine respiratory disease cases. Veterinary Microbiology. 192:186-193.
Comparison of the diagnostic performance of bacterial culture of nasopharyngeal swab and bronchoalveolar lavage fluid samples obtained from calves with bovine respiratory disease - (Peer Reviewed Journal)
Capik, S.F., White, B.J., Lubbers, B.V., Apley, M.D., DeDonder, K.D., Larson, R.L., Harhay, G.P., Chitko-McKown, C.G., Harhay, D.M., Kalbfleisch, T.S., Schuller, G., Clawson, M.L. 2017. Comparison of the diagnostic performance of bacterial culture of nasopharyngeal swab and bronchoalveolar lavage fluid samples obtained from calves with bovine respiratory disease. American Journal of Veterinary Research. 78(3):350-358.
Haptoglobin concentrations from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated - (Abstract Only)
Chitko-McKown, C.G., Workman, A.M., DeDonder, K.D., Bennett, G.L., Apley, M.D., Harhay, G.P., Harhay, D.M., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Clawson, M.L. 2016. Haptoglobin concentrations from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated. International Veterinary Immunology Symposium, August 16-19, 2016, Broadbeach, Australia. Abstract #107.
Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources Reprint Icon - (Peer Reviewed Journal)
Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2016. Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources. Genome Announcements. Vol. 4(3): e00447-16. doi: 10.1128/genomeA.00447-16.
Identification of the pan and core genomes for Mannheimia haemolytica genotypes 1 and 2 - (Abstract Only)
Clawson, M.L., Schuller, G., Dickey, A.M., Bono, J.L., Murray, R.W., Sweeney, M.T., Apley, M.D., DeDonder, K.D., Capik, S.F., Larson, R.L., Lubbers, B.V., White, B.J., Harhay, G.P., Heaton, M.P., Chitko-McKown, C.G., Harhay, D.M., Smith, T.P. 2016. Identification of the pan and core genomes for Mannheimia haemolytica genotypes 1 and 2. American Society for Microbiology 2016 Meeting, July 16-20, 2016, Boston, MA. Poster No. Friday-060.
Genome sequencing, metabolic and antibiotic resistance phenotyping of diverse nasopharyngeal bacteria isolated from cattle in an epidemiological study of bovine respiratory disease - (Abstract Only)
Harhay, G.P., Harhay, D.M., Bono, J.L., Smith, T.P., Heaton, M.P., Clawson, M.L., Chitko-McKown, C.G., Capik, S.F., DeDonder, K.D., Apley, M.D., Lubbers, B.V., White, B.J., Larson, R.L. 2016. Genome sequencing, metabolic and antibiotic resistance phenotyping of diverse nasopharyngeal bacteria isolated from cattle in an epidemiological study of bovine respiratory disease [abstract]. American Society for Microbiology 2016 Meeting, July 16-20, 2016, Boston, MA. Poster No. Friday-057.
Resolving bovine viral diarrhea virus subtypes from persistently infected US beef calves with complete genome sequence - (Peer Reviewed Journal)
Workman, A.M., Heaton, M.P., Harhay, G.P., Smith, T.P.L., Grotelueschen, D.M., Sjeklocha, D.B., Brodersen, B.W., Petersen, J.L., Chitko-McKown, C.G. 2016. Resolving bovine viral diarrhea virus subtypes from persistently infected U.S. beef calves with complete genome sequence. Journal of Veterinary Diagnostic Investigation. 28(5):519-528.
Mobilome differences between Salmonella enterica serovars Anatum and Typhimurium isolated from cattle and humans and potential impact on virulence - (Abstract Only)
Nguyen, S.V., Bono, J.L., Smith, T.P.L., Kelley, C.M., Wang, R., Harhay, G.P., Harhay, D.M. 2016. Mobilome differences between Salmonella enterica serovars Anatum and Typhimurium isolated from cattle and humans and potential impact on virulence. [Abstract] In proceedings: 60th Wind River Conference on Procaryotic Biology, 2016 June 1-5. Estes Park, Colorado. p. 67.
Complete closed genome sequences of Salmonella enterica subsp. enterica serotypes Anatum, Montevideo, Typhimurium and Newport, isolated from beef, cattle, and humans Reprint Icon - (Peer Reviewed Journal)
Harhay, D.M., Bono, J.L., Smith, T.P., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Harhay, G.P. 2016. Complete closed genome sequences of Salmonella enterica subsp. enterica serotypes Anatum, Montevideo, Typhimurium and Newport, isolated from beef, cattle, and humans. Genome Announcements. 4(1):e01683-15. doi:10.1128/genomeA.01683-15.
A searchable, whole genome resource designed for protein variant analysis in diverse lineages of U.S. beef cattle - (Abstract Only)
Heaton, M.P., Kalbfleisch, T.S., Carnahan, J.K., Smith, T.P.L., Harhay, G.P., Simpson, B., Qiu, J. 2015. A searchable, whole genome resource designed for protein variant analysis in diverse lineages of U.S. beef cattle [abstract]. International Plant and Animal Genome XXIV Conference Proceedings, January 9-13, 2016, San Diego, CA. Poster #P0506.
Effects of pseudorabies virus infection on the tracheobronchial lymph node transcriptome - (Peer Reviewed Journal)
Miller, L.C., Bayles, D.O., Zanella, E.L., Lager, K.M., 2016. Effects of pseudorabies virus infection on the tracheobronchial lymph node transcriptome. Bioinformatics and Biology Insights. 9(Suppl 2):25-36.
Comparison of serum PTX3 from shipping through sickness and recovery in cattle - (Abstract Only)
Chitko-McKown, C.G., Workman, A.M., DeDonder, K.D., Bennett, G.L., Apley, M.D., Harhay, G.P., Harhay, D.M., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Clawson, M.L. 2015. Comparison of serum PTX3 from shipping through sickness and recovery in cattle [Abstract]. Conference of Research Workers in Animal Diseases, December 6-8, 2015, Chicago, IL. Abstract #070P.
Full-length coding sequence for 12 bovine viral diarrhea virus isolates from persistently infected cattle in a feedyard in Kansas - (Peer Reviewed Journal)
Workman, A.M., Harhay, G.P., Heaton, M.P., Grotelueschen, D.M., Sjeklocha, D.B., Smith, T.P.L. 2015. Full-length coding sequence for 12 bovine viral diarrhea virus isolates from persistently infected cattle in a feedyard in Kansas. Genome Announcements. 3(3):e00487-15. doi:10.1128/genomeA.00487-15.
Comparative analysis of twenty-four complete aenome aequences of Salmonella enterica Serotypes Anatum, Montevideo, Typhimurium and Newport isolated from ground beef or asymptomatic cattle on farm or at harvest - (Abstract Only)
Harhay, D.M., Bono, J.L., Smith, T.P., Kalbfleisch, T., Kelley, C., Wang, R., Harhay, G.P. 2015. Comparative analysis of twenty-four complete aenome aequences of Salmonella enterica Serotypes Anatum, Montevideo, Typhimurium and Newport isolated from ground beef or asymptomatic cattle on farm or at harvest [abstract]. American Society for Microbiology 115th General Meeting. Poster No. 397, Session 029.
Complete closed genome sequences of a Mannheimia haemolytica serotype A1 leukotoxin deletion mutant and its wild type parent strain - (Peer Reviewed Journal)
Heaton, M.P., Harhay, G.P., Smith, T.P.L., Bono, J.L., Chitko-McKown, C.G. 2015. Complete closed genome sequences of a Mannheimia haemolytica serotype A1 leukotoxin deletion mutant and its wild-type parent strain. Genome Announcements. 3(3):e00417-15. DOI: 10.1128/genomeA.00417-15.
Joint analysis of bacterial DNA methylation, predicted promoter and regulation motifs for biological significance - (Abstract Only)
Harhay, G.P., Anton, B.P., Smith, T.P.L. [abstract]. 2015. Joint analysis of bacterial DNA methylation, predicted promoter and regulation motifs for biological significance. American Society for Microbiology 2015, 115th General Meeting, May 30-June 2, 2015, New Orleans, LA. Poster #3468.
Identification of a mannheimia haemolytica genetic subtype that causes bovine respiratory disease - (Abstract Only)
Clawson, M.L., Murray, R.W., Sweeney, M.T., Apley, M.D., DeDonder, K.D., Capik, S., Larson, R., Lubbers, B.V., Kalbfleisch, T.S., Schuller, G., Harhay, G.P., Heaton, M.P., Chitko-McKown, C.G., Workman, A.M., Harhay, D.M., Bono, J.L., Smith, T.P.L. [abstract]. 2015. Identification of a mannheimia haemolytica genetic subtype that causes bovine respiratory disease. American Society for Microbiology 2015, 115th General Meeting, May 30-June 2, 2015, New Orleans, LA. Poster #1410.
Genomic comparison of Salmonella enterica serotypes commonly associated with cattle and beef – Analysis of variation in virulence potential - (Abstract Only)
Harhay, D.M., Bono, J.L., Wang, R., Smith, T.P., Harhay, G.P., Kalbfleisch, T., Wheeler, T.L. 2015. Genomic comparison of Salmonella enterica serotypes commonly associated with cattle and beef – Analysis of variation in virulence potential. [Abstract]. Beef Industry Safety Summit. http://www.bifsco.org/2015researchsessionabstracts.aspx.
Cytokine profiles from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated - (Abstract Only)
Chitko-McKown, C.G., DeDonder, K.D., Bennett, G.L., Apley, M.D., Harhay, G.P., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Workman, A.M. 2014. Cytokine profiles from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated [abstract]. Conference on Research Workers in Animal Disease, December 7-9, 2014, Chicago, Illinois. Paper No. 148.
Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses - (Peer Reviewed Journal)
Miller, L.C., Jiang, Z., Sang, Y., Harhay, G.P., Lager, K.M. 2014. Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses. Veterinary Immunology and Immunopathology. 159(3-4):180-191.
Complete closed genome sequences of four Mannheimia varigena isolates from cattle with shipping fever - (Peer Reviewed Journal)
Harhay, G.P., Murray, R.W., Lubbers, B., Griffin, D., Koren, S., Phillippy, A.M., Harhay, D.M., Bono, J.L., Clawson, M.L., Heaton, M.P., Chitko-McKown, C.G., Smith, T.P. 2014. Complete closed genome sequences of four Mannheimia varigena isolates from cattle with shipping fever. Genome Announcements. 2(1):e00088-14.
Complete closed genome sequences of three Bibersteinia trehalosi nasopharyngeal isolates from cattle with shipping fever - (Peer Reviewed Journal)
Harhay, G.P., McVey, D.S., Koren, S., Phillippy, A.M., Bono, J.L., Harhay, D.M., Clawson, M.L., Heaton, M.P., Chitko-McKown, C.G., Korlach, J., Smith, T.P. 2014. Complete closed genome sequences of three Bibersteinia trehalosi nasopharyngeal isolates from cattle with shipping fever. Genome Announcements. 2(1):e00084-14.
Characterization of microbiomes related to respiratory disease in cattle - (Abstract Only)
Smith, T.P., Kim, M.S., Myer, P.R., McDaneld, T.G., Kuehn, L.A., Clawson, M.L., Harhay, D.M., Harhay, G.P. 2014. Characterization of microbiomes related to respiratory disease in cattle [Abstract]. Proceedings of the Plant and Animal Genome XXII Conference, January 11-15, 2014, San Diego, CA. Poster W759.
Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses - (Abstract Only)
Miller, L.C., Jiang, Z., Sang, Y., Harhay, G.P., Lager, K.M. 2014.Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses. International Plant & Animal Genome XXII. P595.
Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses - (Abstract Only)
Reducing assembly complexity of microbial genomes with single-molecule sequencing - (Peer Reviewed Journal)
Koren, S., Harhay, G.P., Smith, T.P.L., Bono, J.L., Harhay, D.M., McVey, D.S., Radune, D., Bergman, N.H., Phillippy, A.M. 2013. Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome Biology. 14:R101. DOI: 10.1186/GB-2013-14-9-R101.
Effects of TMEM154 haplotypes 1 and 3 on susceptibility to ovine progressive pneumonia virus following natural exposure in sheep - (Peer Reviewed Journal)
Leymaster, K.A., Chitko-McKown, C.G., Clawson, M.L., Harhay, G.P., Heaton, M.P. 2013. Effects of TMEM154 haplotypes 1 and 3 on susceptibility to ovine progressive pneumonia virus following natural exposure in sheep. Journal of Animal Science. 91(11):5114-5121.
Small ruminant lentivirus genetic subgroups associate with sheep TMEM154 genotypes - (Peer Reviewed Journal)
Sider, L.H., Heaton, M.P., Chitko-Mckown, C.G., Harhay, G.P., Smith, T.P.L., Leymaster, K.A., Laegreid, W.W., and Clawson, M.L. 2013. Small ruminant lentivirus genetic subgroups associate with sheep TMEM154 genotypes. Veterinary Research. 44:64. DOI: 10.1186/1297-9716-44-64.
The methylome and virulence of bovine respiratory disease bacterial pathogens - ()
Harhay, G.P. 2013. The methylome and virulence of bovine respiratory disease bacterial pathogens. American Society for Microbiology. Presentation in WS-24, Studying Whole-Genome Microbial Epigenetics.
Complete closed genome sequences of Mannheimia haemolytica serotypes A1 and A6 isolated from cattle - (Peer Reviewed Journal)
Harhay, G.P., Koren, S., Phillippy, A.M., McVey, D.S., Kuszak, J., Clawson, M.L., Harhay, D.M., Heaton, M.P., Chitko-McKown, C.G., Smith, T.P.L. 2013. Complete closed genome sequences of Mannheimia haemolytica serotypes A1 and A6 isolated from cattle. Genome Announcements. 1(3):e00188-13. DOI: 10.1128/GENOMEA.00188-13.
Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with influenza A virus - (Abstract Only)
Miller, L.C., Jiang, Z., Sang, Y., Harhay, G.P., Lager, K.M. 2013. Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with influenza A virus. Second Annual Great Plains Emerging Infectious Diseases Conference. p. 23.
Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses - (Abstract Only)
Reactomes of porcine alveolar macrophages infected with porcine reproductive and respiratory syndrome virus - (Peer Reviewed Journal)
Jiang, Z., Zhou, X., Michal, J.J., Wu, X.-L., Zhang, L., Zhang, M., Ding, B., Liu, B., Manoranjan, V.S., Neill, J.D., Harhay, G.P., Kehrli, Jr., M.E., Miller, L.C. 2013. Reactomes of porcine alveolar macrophages infected with porcine reproductive and respiratory syndrome virus. PLoS One. 8(3):e59229.
Salmonella virulence, genomics and interactions with the immune system - (Proceedings)
Harhay, D.M., Smith, T.P., Harhay, G.P., Bono, J.L., Bosilevac, J.M. 2013. Salmonella virulence, genomics and interactions with the immune system. Meeting Abstract. The Beef Industry Safety Summit, March 13-15, 2013, Dallas Texas, FLASHDRIVE.
Genetic testing for TMEM154 mutations associated with lentivirus susceptibility in sheep - (Peer Reviewed Journal)
Heaton, M.P., Kalbfleisch, T.S., Petrik, D.T., Simpson, B., Kijas, J.W., Clawson, M.L., Chitko MckKown, C.G., Harhay, G.P., Leymaster, K.A. 2013. Genetic testing for TMEM154 mutations associated with lentivirus susceptibility in sheep. PLoS One. 8(2):e55490. doi:10.1371/journal.pone.0055490.
Application of SMRT genome sequencing to reveal the methylomes of bacteria associated with respiratory disease outbreaks in beef cattle - (Abstract Only)
Harhay, G.P., McVey, D.S., Korlach, J., Roberts, R., Chitko-McKown, C.G., Clawson, M.L., Heaton, M.P., Harhay, D.M., Smith, T.P.L., Anton, B.P. 2013. Application of SMRT genome sequencing to reveal the methylomes of bacteria associated with respiratory disease outbreaks in beef cattle [Abstract]. Advances in Genome Biology and Technology Conference, February 20-23, 2013, Marco Island, FL. Page 99.
Genetic testing for TMEM154 mutations associated with lentivirus susceptibility in sheep - (Abstract Only)
Heaton, M.P., Kalbfleisch, T., Petrik, D.T., Simpson, B., Kijas, J., Clawson, M.L., Chitko-McKown, C.G., Leymaster, K.A., Harhay, G.P. 2013. Genetic testing for TMEM154 mutations associated with lentivirus susceptibility in sheep [abstract]. International Plant & Animal Genome XXI Conference. No. P0625.
Comparative transcriptome response in swine tracheobronchial lymph nodes to viral infection - (Abstract Only)
Miller, L.C., Harhay, G.P., Kehrli, Jr., M.E., Lager, K.M. 2013. Comparative transcriptome response in swine tracheobronchial lymph nodes to viral infection [abstract]. International Plant & Animal Genome XXI, January 12-16, 2013, San Diego, California. Paper No. W756.
Next-generation sequencing of the porcine skeletal muscle transcriptome for computational prediction of microRNA gene targets - (Peer Reviewed Journal)
McDaneld, T.G., Smith, T.P.L., Harhay, G.P., and Wiedmann, R.T. 2012. Next-generation sequencing of the porcine skeletal muscle transcriptome for computational prediction of microRNA gene targets. PLoS One. 7(7):e42039. doi:10.1371/journal.pone.0042039.
Phylogenetic clades of ovine progressive pneumonia virus (OPPV) associate with sheep TMEM154 genotypes - (Abstract Only)
Sider, L.H., Clawson, M.L., Heaton, M.P., Chitko Mckown, C.G., Harhay, G.P., Smith, T.P.L., Leymaster, K.A. 2012. Phylogenetic clades of ovine progressive pneumonia virus (OPPV) associate with sheep TMEM154 genotypes. The 58th Genomic Congress, September 11-14, 2012. Foz de Iguacu, Brazil. Poster No. GM001.
Comparison of bacterial communities in faeces of beef cattle fed diets containing corn and wet distillers grain with solubles - (Peer Reviewed Journal)
Durso, L.M., Wells, J., Harhay, G.P., Rice, W.C., Kuehn, L.A., Bono, J.L., Shackelford, S.D., Wheeler, T.L., Smith, T.P. 2012. Comparison of bacterial communities in faeces of beef cattle fed diets containing corn and wet distillers grain with solubles. Letters in Applied Microbiology. 55(2):109-14. DOI: 10.1111/J.1472-765X.2012.03265.X.
Reduced OPP susceptibility in naturally exposed lambs with homozygous TMEM154 K35 genotypes - (Abstract Only)
Chitko Mckown, C.G., Leymaster, K.A., Heaton, M.P., Clawson, M.L., Harhay, G.P. 2012. Reduced OPP susceptibility in naturally exposed lambs with homozygous TMEM154 K35 genotypes [abstract]. Immunology 2012. The 99th Annual Meeting of the American Association for Immunology, May 4-8, 2012, Boston, Massachusetts. Page 275. Abstract No. 170.3. Poster No. P1246.
Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans - (Peer Reviewed Journal)
Bono, J.L., Smith, T.P., Keen, J.E., Harhay, G.P., McDaneld, T.G., Mandrell, R.E., Jung, W., Besser, T.E., Gerner-Smidt, P., Bielaszewska, M., Karch, H., Clawson, M.L. 2012. Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans. Molecular Biology and Evolution. 29(8):2047-2062. doi: 10.1093/molbev/mss072.
Application of combined SMRT and long-read pyrosequencing to produce reference genome sequences of bacteria associated with respiratory disease outbreaks in beef cattle - (Abstract Only)
Harhay, G.P., Mcvey, D.S., Kitzmiller, A., Clamp, M., Osborne, B., Dwan, C., Zhang, J., Sisneros, N., Wang, S., Peluso, P., Bono, J.L., Chitko Mckown, C.G., Clawson, M.L., Heaton, M.P., Harhay, D.M., Smith, T.P.L. [abstract]. 2012. Application of combined SMRT and long-read pyrosequencing to produce reference genome sequences of bacteria associated with respiratory disease outbreaks in beef cattle. Advances in Genome Biology and Technology, February 15-18, 2012, Marco Island, Florida. Poster No. 129.
Enabling next-gen sequencing and analysis at the USDA-ARS U.S. Meat Animal Research Center with MiniLIMS - (Abstract Only)
Reduced lentivirus susceptibility in sheep with TMEM154 mutations - (Peer Reviewed Journal)
Heaton, M.P., Clawson, M.L., Chitko-Mckown, C.G., Leymaster, K.A., Smith, T.P.L., Harhay, G.P., White, S.N., Herrmann-Hoesing, L.M., Mousel, M.R., Lewis, G.S., Kalbfleisch, T.S., Keen, J.E., Laegreid, W.W. 2012. Reduced lentivirus susceptibility in sheep with TMEM154 mutations. PLoS Genetics. 8(1): e1002467. DOI: 10.1371/journal.pgen.1002467.
Comparison of immune transcriptome response following infection with PRRSV, PCV2 and SIV - (Abstract Only)
Miller, L.C., Harhay, G., Kehrli, M., Lager, K. 2011. Comparison of immune transcriptome response following infection with PRRSV, PCV2 and SIV [abstract]. 2011 International PRRS Symposium. Paper No. 51. p. 68.
Immune transcriptome response to PRRSV infection - (Abstract Only)
Miller, L.C., Harhay, G.P., Lager, K.M., Kehrli, Jr., M.E. 2011. Immune transcriptome response to PRRSV infection [abstract]. XIIth International Nidovirus Symposium. p. 79.
Gene expression response of multidrug resistant Salmonella enterica serovar Newport to simulated multiple hurdle beef carcass processing interventions - (Abstract Only)
Harhay, D.M., Smith, T.P., Harhay, G.P. 2011. Gene expression response of multidrug resistant Salmonella enterica serovar Newport to simulated multiple hurdle beef carcass processing interventions. In: Proceedings of the American Society for Microbiology. 111th General Meeting of the American Society for Microbiology, May 21-24, 2011, New Orleans, Louisiana. 2011 CDROM. Meeting Abstract K-2692.
Towards an understanding of how Salmonella potentially evades beef carcass processing interventions - (Abstract Only)
Brichta-Harhay, D.M., Gragg, S.E., Smith, T.P.L., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L., Brashears, M.M., Brooks, J.C., Brown, T.R., Harhay, G.P., Loneragan, G.H. 2011. Towards an understanding of how Salmonella potentially evades beef carcass processing interventions [abstract]. 2011 Beef Industry Safety Summit. Abstract No. 2.
Biosurveillance at the United States Meat Animal Research Center - (Abstract Only)
Harhay, G.P., Harhay, D.M., Chitko Mckown, C.G., Smith, T.P. 2011. Biosurveillance at the United States Meat Animal Research Center [abstract]. Global Biosurveillance: Enabling Science and Technology 2nd Biothreat Nonproliferation Conference. Abstract No. 12.
In depth global analysis of transcript abundance levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus - (Peer Reviewed Journal)
Miller, L.C., Neill, J.D., Harhay, G.P., Lager, K.M., Laegreid, W.W., Kehrli, Jr., M.E. 2010. In-depth global analysis of transcript abundance levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus. Advances in Virology. 2010:Article 864181. Available: http://downloads.hindawi.com/journals/av/2010/864181.pdf.
Virulence-associated and antibiotic resistance genes of microbial populations in cattle feces analyzed using a metagenomic approach - (Peer Reviewed Journal)
Durso, L.M., Harhay, G.P., Bono, J.L., Smith, T.P. 2011. Virulence-associated and antibiotic resistance genes of microbial populations in cattle feces analyzed using a metagenomic approach. Journal of Microbiological Methods. 84: 278-282.
Bacterial community analysis of beef cattle feedlots reveals that pen surface is distinct from feces - (Peer Reviewed Journal)
Durso, L.M., Harhay, G.P., Smith, T.P., Bono, J.L., Desantis, T.Z., Clawson, M.L. 2011. Bacterial community analysis of beef cattle feedlots reveals that pen surface is distinct from feces. Foodborne Pathogens and Disease. 8(5):647-649. Available: DOI: 10.1089/fpd.2010.0774.
An atlas of bovine gene expression reveals novel distinctive tissue characteristics and evidence for improving genome annotation - (Peer Reviewed Journal)
Harhay, G.P., Smith, T.P.L., Alexander, L.J., Haudenschild, C.D., Keele, J.W., Matukumalli, L.K., Schroeder, S.G., Van Tassell, C.P., Gresham, C.R., Bridges, S.M., Burgess, S.C., Sonstegard, T.S. 2010. An atlas of bovine gene expression reveals novel distinctive tissue characteristics and evidence for improving genome annotation. Genome Biology [online serial]. 11:R102.
Transcript Expression Analysis in Tracheobronchial Lymph Nodes of Pseudorabies Virus Infected Pigs - (Abstract Only)
Miller, L.C., Harhay, G.P., Bayles, D.O., Zanella, E.L., Lager, K.M. 2010. Transcript expression analysis in tracheobronchial lymph nodes of pseudorabies virus infected pigs [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 115P.
Transcript Expression Analysis in Tracheobronchial Lymph Nodes of Pseudorabies Virus Infected Pigs - (Abstract Only)
Miller, L.C., Harhay, G.P., Bayles, D.O., Zanella, E.L., Lager, K.M. 2010. Transcript expression analysis in tracheobronchial lymph nodes of pseudorabies virus infected pigs [abstract]. 45th Meeting of the United States-Japan Cooperative Program in Natural Resources Panel of Animal and Avian Health. p. 11.
Transcript Expression Analysis in Tracheobronchial Lymph Nodes of Pseudorabies Virus Infected Pigs - (Abstract Only)
Miller, L.C., Harhay, G.P., Bayles, D.O., Zanella, E.L., Lager, K.M. 2010. Transcript Expression Analysis in Tracheobronchial Lymph Nodes of Pseudorabies Virus Infected Pigs [abstract]. 32nd Conference for the International Society for Animal Genetics. Paper No. P105.
Animal-to-Animal Variation in Fecal Microbial Diversity among Beef Cattle - (Peer Reviewed Journal)
Durso, L.M., Harhay, G.P., Smith, T.P.L., Bono, J.L., Desantis, T.Z., Harhay, D.M., Andersen, G.L., Keen, J.E., Laegreid, W.W., Clawson, M.L. 2010. Animal-to-Animal Variation in Fecal Microbial Diversity among Beef Cattle. Applied and Environmental Microbiology. 76(14):4858-4862.
Comparison of cattle fecal communities from animals fed diets containing corn and distiller's grains - (Abstract Only)
Durso, L.M., Harhay, G.P., Wells, J., Bono, J.L., Riethoven, J.M., Shackelford, S.D., Wheeler, T.L., Smith, T.P. 2010. Comparison of cattle fecal communities from animals fed diets containing corn and distiller's grains. PS.25.018, Abstract #393A. Abstract for Microbial Ecology International Symposium, Seattle, WA, Aug. 22-27, 2010. Abstract Book, Pg. 128
In Depth Global Analysis of Transcript Abundance Levels Following Infection with Porcine Reproductive and Respiratory Syndrome Virus - (Abstract Only)
Miller, L.C., Harhay, G.P., Lager, K.M., Neill, J.D., Kehrli, Jr., M.E. 2010. In-Depth Global Analysis of Transcript Abundance Levels Following Infection with Porcine Reproductive and Respiratory Syndrome Virus [abstract]. In: Proceedings of the 9th International Positive Strand RNA Virus Symposium, May 17-21, 2010, Atlanta, Georgia. p. 54.
De novo Reconstruction of the Pig Skeletal Muscle Transcriptome for Identification of MicroRNA Gene Targets - (Abstract Only)
McDaneld, T.G., Wiedmann, R.T., Harhay, G.P., Smith, T.P.L. 2010. De novo Reconstruction of the Pig Skeletal Muscle Transcriptome for Identification of MicroRNA Gene Targets (abstract). In: Proceedings of the Advances in Genome Biology & Technology Conference, February 24-27,2010, Marco Island, Florida. p. 114.
A Digital Gene Expression-Based Bovine Gene Atlas Evaluating 92 Adult, Juvenile and Fetal Cattle Tissues - (Abstract Only)
Harhay, G.P., Alexander, L.J., Smith, T.P.L., Matukumalli, L.K., Schroeder, S.G., Sonstegard, T.S. 2010. A Digital Gene Expression-Based Bovine Gene Atlas Evaluating 92 Adult, Juvenile and Fetal Cattle Tissues [abstract]. In: Proceedings of the Advances in Genome Biology & Technology Conference, February 24-27, 2010, Marco Island, Florida. p. 67.
Information Theoretical Analysis of a Bovine Gene Atlas Reveals Chromosomal Regions with Tissue Specific Gene Expression. - (Abstract Only)
Sonstegard, T.S., Harhay, G.P., Alexander, L.J., Schroeder, S.G., Keele, J.W., Matukumalli, L., Bridges, S., Burgess, S., Van Tassell, C.P., Smith, T.P. 2010. Information Theoretical Analysis of a Bovine Gene Atlas Reveals Chromosomal Regions with Tissue Specific Gene Expression. Plant and Animal Genome VX Conference Abstracts.
The Genome Sequence of Taurine Cattle: A Window to Ruminant Biology and Evolution - (Peer Reviewed Journal)
Elsik, C.G., Gibbs, R., Skow, L., Tellam, R., Weinstock, G., Worley, K., Kappes, S.M., Green, R.D., Alexander, L.J., Bennett, G.L., Carroll, J.A., Chitko Mckown, C.G., Hamernik, D.L., Harhay, G.P., Keele, J.W., Liu, G., Macneil, M.D., Matukumalli, L.K., Rijnkels, M., Roberts, A.J., Smith, T.P., Snelling, W.M., Stone, R.T., Waterman, R.C., White, S.N. 2009. The Genome Sequence of Taurine Cattle: A Window to Ruminant Biology and Evolution. Science. 324:522-528.
Bacterial Communities Associated with Shiga-Toxigenic E. coli O157:H7 (STEC O157) in Beef Cattle Feedlot Environments - (Abstract Only)
Durso, L.M., Harhay, G.P., Bono, J.L., Smith, T.P., Laegreid, W.W., Keen, J.E., Clawson, M.L. 2009. Bacterial Communities Associated with Shiga-Toxigenic E. coli O157:H7 (STEC O157) in Beef Cattle Feedlot Environments [abstract]. Microbial Ecology 57(3):569.
Prevalence of the prion gene E211K variant in U.S. cattle - (Peer Reviewed Journal)
Heaton, M.P., Keele, J.W., Harhay, G.P., Richt, J., Koohmaraie, M., Wheeler, T.L., Shackelford, S.D., Casas, E., King, D.A., Sonstegard, T.S., Van Tassell, C.P., Neibergs, H.L., Chase, C.C., Kalbfleisch, T.S., Smith, T.P., Clawson, M.L., Laegreid, W.W. 2008. Prevalence of the prion gene E211K variant in U.S. cattle. BioMed Central (BMC) Veterinary Research [journal online]. 4:25. Available: (http://www.biomedcentral.com/1746-6148/4/25).
A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle - (Peer Reviewed Journal)
Clawson, M.L., Heaton, M.P., Keele, J.W., Smith, T.P., Harhay, G.P., Richt, J., Laegreid, W.W. 2008. A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle. BMC Research Notes [journal online]. 1:32. Available: http://www.biomedcentral.com/1756-0500/1/32.
In depth global analysis of gene expression levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus - (Abstract Only)
Miller, L., Harhay, G., Lager, K., Kehrli Jr., M., Laegreid, W., Neill, J. 2008. In depth global analysis of gene expression levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus [abstract]. ARK-Genomics Conference 2008: 3rd International Symposium on Animal Functional Genomics. Paper No. ISAFG-P22. p. 38.
Microbial Community Structure of Feedlot Cattle Feces - (Abstract Only)
Durso, L.M., Harhay, G.P., Clawson, M.L., Laegreid, W.W., Bono, J.L., Keen, J.E., Smith, T.P. Microbial community structure of feedlot cattle feces. In: Proceedings of the 108th General Meeting of the American Society for Microbiology, June 1-5, 2008, Boston, Massachusetts. 2008 CDROM.
Effect of porcine reproductive and respiratory syndrome virus on porcine alveolar macrophage function as determined using serial analysis of gene expression (SAGE) - (Peer Reviewed Journal)
Miller, L.C., Harhay, G.P., Lager, K.M., Smith, T.P., Neill, J.D. 2008. Effect of porcine reproductive and respiratory syndrome virus on porcine alveolar macrophage function as determined using serial analysis of gene expression (SAGE). Developments in Biologicals. 132:169-174.
Gene Expression Profiling of Porcine Reproductive and Respiratory Syndrome Virus Infected Porcine Alveolar Macrophages - (Abstract Only)
Miller, L.C., Harhay, G.P., Lager, K.M., Smith, T.P., Neill, J.D. 2007. Gene expression profiling of PRRSV-infected porcine alveolar macrophages [abstract]. Porcine Reproductive and Respiratory Syndrome International Symposium. Paper No. 47.
Integrating the USMARC genetic map for the pig with the pig physical map - (Abstract Only)
Wiedmann, R.T., Freking, B.A., Nonneman, D.J., Snelling, W.M., Keele, J.W., Harhay, G.P., Rohrer, G.A. Integrating the USMARC genetic map for the pig with the pig physical map. 2008. Annual International Plant & Animal Genome Conference. Poster No. P565, pg 259.
Prevalence of the prion gene E211K variant in U.S. cattle - (Abstract Only)
Heaton, M.P., Keele, J.W., Harhay, G.P., Richt, J., Koohmaraie, M., Wheeler, T.L., Shackelford, S.D., Casas, E., King, D.A., Sonstegard, T.S., Van Tassell, C.P., Neibergs, H.L., Chase, C.C., Kalbfleisch, T.S., Smith, T.P., Clawson, M.L., Laegreid, W.W. 2008. Prevalence of the prion gene E211K variant in U.S. cattle (abstract). Plant and Animal Genome XVI Conference. Poster No. P538.
Description and analysis of the Bovine Gene Atlas - An extensive compendium of bovine transcript profiles - (Abstract Only)
Harhay, G.P., Keele, J.W., Smith, T.P., Alexander, L.J., Matukumalli, L.K., Schroeder, S.G., Liu, G., Van Tassell, C.P., Sonstegard, T.S. 2008. Description and analysis of the Bovine Gene Atlas - An extensive compendium of bovine transcript profiles (abstract). Plant and Animal Genome XVI Conference. Poster No. P516.
Effect of porcine reproductive and respiratory syndrome virus on porcine alveolar macrophage function as determined using serial analysis of gene expression (SAGE) - (Abstract Only)
Neill, J.D., Miller, L.C., Harhay, G.P., Lager, K.M. 2007. Effect of porcine reproductive and respiratory syndrome virus on porcine alveolar macrophage function as determined using serial analysis of gene expression (SAGE) [abstract]. International Symposium on Animal Genomics for Animal Health. Paper No. PA-070. p. 58.
Creation of a Gene Atlas in cattle using sequence-based transcriptional profiling - (Abstract Only)
Sonstegard, T.S., Keele, J.W., Harhay, G.P., Smith, T.P., Matukumalli, L.K., Van Tassell, C.P., Liu, G., Alexander, L.J. 2007. Creation of a Gene Atlas in cattle using sequence-based transcriptional profiling. [abstract] Joint Abstracts of the American Dairy Science and Society of Animal Science.
Prion gene haplotypes of U.S. cattle - (Peer Reviewed Journal)
Clawson, M.L., Heaton, M.P., Keele, J.W., Smith, T.P., Harhay, G.P., Laegreid, W.W. 2006. Prion gene haplotypes of U.S. cattle. BioMed Central (BMC) Genetics. 7:51.
An updated USMARC genetic map for the pig integrated with the pig physical map - (Abstract Only)
Rohrer, G.A., Snelling, W.M., Freking, B.A., Nonneman, D.J., Harhay, G.P., Keele, J.W. 2007. An updated USMARC genetic map for the pig integrated with the pig physical map [abstract]. Plant and Animal Genome XV Conference. Poster No. W421.
Polymorphisms and haplotype structure of bovine PRND (doppel) and PRNT - (Abstract Only)
Laegreid, W.W., Clawson, M.L., Smith, T.P., Harhay, G.P., Keele, J.W., Heaton, M.P. 2007. Polymorphisms and haplotype structure of bovine PRND (doppel) and PRNT [abstract]. Plant and Animal Genome XV Conference. Poster No. P535.
A rapid sequence-based method for comprehensive polymorphism identification within a 25.2-kb region of the bovine prion gene in BSE-affected cattle - (Abstract Only)
Clawson, M.L., Heaton, M.P., Keele, J.W., Smith, T.P., Harhay, G.P., Laegreid, W.W. 2007. A rapid sequence-based method for comprehensive polymorphism identification within a 25.2-kb region of the bovine prion gene in BSE-affected cattle [abstract]. Plant and Animal Genome XV Conference. Poster No. P533.
A marker set for parentage-based DNA traceback in beef and dairy cattle - (Abstract Only)
Heaton, M.P., Snelling, W.M., Smith, T.P., Keele, J.W., Harhay, G.P., Wiedmann, R.T., Bennett, G.L., Freking, B.A., Van Tassell, C.P., Sonstegard, T.S., Gasbarre, L.C., Moore, S.S., Murdoch, B., Mckay, S.D., Kalbfleisch, T., Laegreid, W.W. 2007. A marker set for parentage-based DNA traceback in beef and dairy cattle [abstract]. Plant and Animal Genome XV Conference. Poster No. P516.
CHARACTERIZATION OF 954 BOVINE FULL-CDS CDNA SEQUENCES - (Peer Reviewed Journal)
Harhay, G.P., Sonstegard, T.S., Keele, J.W., Heaton, M.P., Clawson, M.L., Snelling, W.M., Wiedmann, R.T., Van Tassell, C.P., Smith, T.P. 2005. Characterization of 954 bovine full-CDS cDNA sequences. Biomed Central (BMC) Genomics 6:166 (11 pp).
IDENTIFICATION OF GENETIC MARKERS FOR FAT DEPOSITION AND MEAT TENDERNESS ON BOVINE CHROMOSOME 5: DEVELOPMENT OF A LOW DENSITY SNP MAP - (Peer Reviewed Journal)
Stone, R.T., Casas, E., Smith, T.P.L., Keele, J.W., Harhay, G.P., Bennett, G.L., Koohmaraie, M., Wheeler, T.L., Shackelford, S.D., Snelling, W.M. 2005. Identification of genetic markers for fat deposition and meat tenderness on bovine chromosome 5: Development of a low-density single nucleotide polymorphism map. Journal of Animal Science. 83:2280-2288.
LINKAGE MAPPING BOVINE EST-BASED SNP - (Peer Reviewed Journal)
Snelling, W.M., Casas, E., Stone, R.T., Keele, J.W., Harhay, G.P., Bennett, G.L., Smith, T.P. 2005. Linkage mapping bovine EST-based SNP. Biomed Central (BMC) Genomics 6:74.
INTEGRATING LINKAGE AND RADIATION HYBRID MAPPING DATA FOR BOVINE CHROMOSOME 15 - (Peer Reviewed Journal)
Snelling, W.M., Gautier, M., Keele, J.W., Smith, T.P., Stone, R.T., Harhay, G.P., Bennett, G.L., Ihara, N., Takasuga, A., Takeda, H., Sugimoto, Y., Eggen, A. Integrating linkage and radiation hybrid mapping data for bovine chromosome 15. Biomed Central (BMC) Genomics. 5:77. 2004.
USE OF BOVINE SNP MARKERS TO VERIFY SAMPLE TRACKING IN BEEF PROCESSING - (Abstract Only)
Heaton, M.P., Keen, J.E., Clawson, M.L., Harhay, G.P., Bauer, N., Shultz, C., Green, B.T., Durso, L.M., Chitko Mckown, C.G., Laegreid, W.W. Use of bovine SNP markers to verify sample tracking in beef processing [abstract]. Plant and Animal Genome Conference Proceedings. p. 124.
USE OF BOVINE SINGLE NUCLEOTIDE POLYMORPHISM MARKERS TO VERIFY SAMPLE TRACKING IN BEEF PROCESSING - (Peer Reviewed Journal)
Heaton, M.P., Keen, J.E., Clawson, M.L., Harhay, G.P., Bauer, N., Schultz, C., Green, B.T., Durso, L.M., Chitko Mckown, C.G., Laegreid, W.W. 2005. Use of bovine single nucleotide polymorphism markers to verify sample tracking in beef processing. Journal of the American Veterinary Medical Association 226(8):1311-1314.
DATABASE DIRECTED FULL-LENGTH CDNA GENERATION USING EST DATA FROM NORMALIZED BOVINE CDNA LIBRARIES - (Abstract Only)
Harhay, G.P., Smith, T.P., Sonstegard, T.S., Clawson, M.L. 2004. Database directed full-length cDNA generation using EST data from normalized bovine cDNA libraries. Proc., 29th International Conference on Animal Genetics (ISAG), Tokyo, Japan. September 11-16, 2004. Abstract A022, p. 37.
DATABASE DIRECTED FULL-LENGTH CDNA GENERATION FROM NORMALIZED BOVINE CDNA LIBRARIES - (Abstract Only)
Harhay, G.P., Sonstegard, T.S., Clawson, M.L., Smith, T.P. 2004. Database directed full-length cDNA generation from normalized bovine cDNA libraries. International Genome Sequencing and Analysis Conference XVI, Washington, DC. September 27-30, 2004. Poster Abstract #49.
PRODUCTION OF EST FROM CDNA LIBRARIES DERIVED FROM IMMUNOLOGICALLY ACTIVATED BOVINE ABOMASUM - ()
Sonstegard, T.S., Van Tassell, C.P., Matukumalli, L.K., Harhay, G.P., Bosak, S., Rubenfield, M., Gasbarre, L.C. 2004. Production of est from cdna libraries derived from immunologically activated bovine abomasum. Germplasm Release.
PRODUCTION OF EST FROM CDNA LIBRARIES DERIVED FROM IMMUNOLOGICALLY ACTIVATED BOVINE SMALL INTESTINE - ()
Sonstegard, T.S., Van Tassell, C.P., Matukumalli, L.K., Harhay, G.P., Bosak, S., Rubenfield, M., Gasbarre, L.C. 2004. Production of est from cdna libraries derived from immunologically activated bovine small intestine. Germplasm Release.
PRODUCTION OF A PORCINE-HUMAN COMPARATIVE MAP BASED ON THE PORCINE GENETIC MAP AND HUMAN GENOME SEQUENCE - (Proceedings)
Rohrer, G.A., Nonneman, D.J., Freking, B.A., Harhay, G.P., Snelling, W.M., Keele, J.W. 2002. Production of a porcine-human comparative map based on the porcine genetic map and human genome sequence. Plant and Animal Genome Conference Proceedings. Abstract P612.
POSITIONAL CANDIDATE GENE SELECTION FROM LIVESTOCK EST DATABASES USING GENE ONTOLOGY - (Peer Reviewed Journal)
Harhay, G.P., Keele, J.W. 2003. Positional candidate gene selection from livestock EST databases using Gene Ontology. Bioinformatics. 19:249-255.
SELECTION AND USE OF SNP MARKERS FOR ANIMAL IDENTIFICATION AND PATERNITY ANALYSIS IN U.S. BEEF CATTLE - (Peer Reviewed Journal)
Mammalian Genome. 2002. v. 13. p. 272-281.
SELECTION AND USE OF SINGLE NUCLEOTIDE POLYMORPHISM (SNP) MARKERS FOR ANIMAL IDENTIFICATION AND PATERNITY ANALYSIS IN U.S. BEEF CATTLE - (Abstract Only)