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James Zhu (PhD)
Foreign Animal Disease Research
Computational Biologist

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Projects
Intervention Strategies to Control and Eradicate Foreign Animal Diseases of Swine
In-House Appropriated (D)
  Accession Number: 441308
Intervention Strategies to Respond, Control, and Eradicate Foot-and-Mouth Disease Virus (FMDV)
In-House Appropriated (D)
  Accession Number: 441364
Determining Immune Cells Draining to Lymph Nodes and the Factors Affecting the Cell Drainage
Non-Assistance Cooperative Agreement (S)
  Accession Number: 446468

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Exploring the molecular basis of vesicular stomatitis virus pathogenesis in swine: insights from expression profiling of primary macrophages infected with M51R mutant virus Reprint Icon - (Peer Reviewed Journal)
Velazquez Salinas, L., Medina, G.N., Valdez, F., Collinson, S., Zarate, S., Zhu, J.J., Rodriguez, L.L. 2023. Exploring the molecular basis of vesicular stomatitis virus pathogenesis in swine: insights from expression profiling of primary macrophages infected with M51R mutant virus. Pathogens. 12(7). Article 896. https://doi.org/10.3390/pathogens12070896.
Mutation of FMDV Lpro residues outside substrate binding domain drives viral attenuation Reprint Icon - (Abstract Only)
Diaz San Segundo, F.C., Azzinaro, P.A., Zhu, J.J., Medina, G.N., De Los Santos, T.B. 2022. Mutation of FMDV Lpro residues outside substrate binding domain drives viral attenuation. Meeting Abstract. https://doi.org/10.3389/fvets.2022.1028077.
Mutation of FMDV Lpro H138 reside outside substrate binding domain drives viral attenuation in vitro and in swine Reprint Icon - (Peer Reviewed Journal)
Azzinaro, P.A., Medina, G.N., Rai, D., Ramirez Medina, E., Spinard Iii, E.J., Zhu, J.J., Rieder, A.E., De Los Santos, T.B., Diaz San Segundo, F.C. 2022. Mutation of FMDV Lpro H138 reside outside substrate binding domain drives viral attenuation in vitro and in swine. Frontiers in Veterinary Science. https://www.frontiersin.org/articles/10.3389/fvets.2022.1028077/full.
African Swine Fever Vaccinology: Why Is It Difficult? Reprint Icon - (Peer Reviewed Journal)
Zhu, J.J. 2022. African Swine Fever Vaccinology: Why Is It Difficult? . Viruses. https://doi.org/10.3390/v14092021.
African Swine Fever Vaccinology: Why Is It Difficult? Reprint Icon - (Peer Reviewed Journal)
Zhu, J.J. 2022. African Swine Fever Vaccinology: Why Is It Difficult?. Viruses. https://doi.org/10.3390/v14092021.
Inferred Causal Mechanisms of Persistent FMDV Infection from Differential Gene Expression during the Transitional Phase from Acute to Persistent Infection Reprint Icon - (Peer Reviewed Journal)
Zhu, J.J., Stenfeldt, C., Bishop, E.A., Canter, J.A., Eschbaumer, M., Rodriguez, L.L., Arzt, J. 2022. Inferred Causal Mechanisms of Persistent FMDV Infection from Differential Gene Expression during the Transitional Phase from Acute to Persistent Infection. Pathogens. 11:8:822. https://doi.org/10.3390/pathogens11080822.
Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry Reprint Icon - (Peer Reviewed Journal)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Neilan, J., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry. Scientific Reports. https://doi.org/10.3390/v14061249.
Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg Reprint Icon - (Abstract Only)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg. Meeting Abstract. https://doi.org/10.3390/v15040915.
Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Pina-Pedrero, S., Zhu, J.J., Rodriguez, F., Borca, M.V., Gladue, D.P. 2021. Deletion of E184L, a putative DIVA target from the pandemic strain of African Swine Fever Virus, produces a reduction in virulence and protection against virulent challenge. Viruses. https://doi.org/10.1128/JVI.01419-21.
Molecular Pathogenesis and Immune Evasion of Vesicular Stomatitis Virus Inferred from Genes Expression Changes in Ex Vivo Porcine Macrophages after Infection Reprint Icon - (Peer Reviewed Journal)
Zhu, J.J., Velazquez-Salinas, L., Canter, J., Rodriguez, L.L. 2021. Molecular Pathogenesis and Immune Evasion of Vesicular Stomatitis Virus Inferred from Genes Expression Changes in Ex Vivo Porcine Macrophages after Infection. Pathogens. https://doi.org/10.3390/pathogens10091134.
Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2021. Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene. Viruses. https://doi.org/10.3390/v13020286.
Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene. Viruses. https://doi.org/10.3390/v13010039.
Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene Reprint Icon - (Peer Reviewed Journal)
Ramierez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Velazquez-Salinas, L., Zhu, J.J., Gladue, D.P., Borca, M.V. 2020. Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene. Viruses. https://doi.org/10.3390/v12101193.
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S.E., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine. Viruses. https://doi.org/10.3390/v12060676.
Mechanisms of Foot-and-Mouth Disease virus persistence inferred from immune-related genes differentially expressed between pharyngeal epithelia of virus carriers and non-carriers Reprint Icon - (Peer Reviewed Journal)
Zhu, J.J., Stenfeldt, C., Bishop, E., Canter, J.C., Eschbaumer, M., Rodriguez, L.L., Arzt, J. 2020. Mechanisms of Foot-and-Mouth Disease virus persistence inferred from immune-related genes differentially expressed between pharyngeal epithelia of virus carriers and non-carriers. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2020.00340.
The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Silva, E., Rai, A., Pruitt, S.E., Vuono, E.A., Velazquez-Salinas, L., Berggren, K.A., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP. Viruses. https://doi.org/10.1128/JVI.02017-19.
Mechanisms of African Swine Fever Virus Pathogenesis and Immune Evasion Inferred from Gene Expression Changes in Infected Swine Macrophages - (Peer Reviewed Journal)
Clearance of a persistent Picornavirus infection is associated with enhanced pro-apoptotic and cellular immune responses Reprint Icon - (Peer Reviewed Journal)
Stenfeldt, C., Eschbaumer, M., Smoliga, G.R., Rodriguez, L.L., Zhu, J.J., Arzt, J. 2017. Clearance of a persistent Picornavirus infection is associated with enhanced pro-apoptotic and cellular immune responses. Scientific Reports. 7:17800. https://doi.org/10.1038/s41598-017-18112-4.
Transcriptomic analysis of persistent infection with foot-and-mouth disease virus in cattle suggests impairment of cell-mediated immunity in the nasopharynx - (Peer Reviewed Journal)
Eschbaumer, M., Stenfeldt, C., Smoliga, G.R., Pacheco, J., Rodriguez, L.L., Li, R.W., Zhu, J.J., Arzt, J. 2016. Transcriptomic analysis of persistent infection with foot-and-mouth disease virus in cattle suggests impairment of cell-mediated immunity in the nasopharynx. PLoS One. 11:e0162750.
Depletion of elongation initiation factor 4E binding proteins by CRISPR/Cas9 genome editing enhances antiviral response in porcine cells - (Peer Reviewed Journal)
Ramirez-Carvajal, L., Singh, N., De Los Santos, T.B., Rodriguez, L.L., Zhu, J.J., Long, C.R. 2015. Depletion of elongation initiation factor 4E binding proteins by CRISPR/Cas9 genome editing enhances antiviral response in porcine cells. Antiviral Research. 125:8-13. doi: 10.1016/j.antiviral.2015.11.002.
Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain Reprint Icon - (Peer Reviewed Journal)
Fernandez-Sainz, I., Zhu, J.J., O'Donnell, V., Diaz San Segundo, F.C., Velazquez, L., De Los Santos, T.B., Borca, M.V. 2015. Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain. Virology. 483:284-290. DOI: 10.1016/j.virol.2015.04.024.
A colorimetric bioassay for high-througput and cost-effectively assessing anti-foot-and-mouth disease virus activity - (Peer Reviewed Journal)
Ramanathan, P., Puckette, M., Hartwig, E.J., Bishop, E.A., Rodriguez, L.L., Grubman, M.J., Zhu, J.J. 2015. A colorimetric bioassay for high-througput and cost-effectively assessing anti-foot-and-mouth disease virus activity. Veterinary Immunology and Immunopathology. 164(1-2):74-78. DOI: 0.1016/j.vetimm.2015.01.005.
Expression of porcine fusion protein IRF7/3(5D) efficiently controls foot-and-mouth disease virus replication - (Peer Reviewed Journal)
Ramirez-Carvajal, L., Long, C.R., Zhu, J.J., Diaz San Segundo, F.C., Rodriguez, L.L., De Los Santos, T.B. 2014. Expression of porcine fusion protein IRF7/3(5D) efficiently controls foot-and-mouth disease virus replication. Journal of Virology. 88(19):11140-11153.
Mechanisms of foot-and-mouth disease virus tropism inferred from differential tissue gene expression - (Peer Reviewed Journal)
Zhu, J.J., Arzt, J., Puckette, M.C., Smoliga, G.R., Pacheco Tobin, J., Rodriguez, L.L. 2013. Mechanisms of foot-and-mouth disease virus tropism inferred from differential tissue gene expression. PLoS One. 8(5):e64119. DOI:10.1371/journal.pone.0064119.
Antiviral activity of bovine type III interferon against foot-and-mouth disease virus - (Peer Reviewed Journal)
Diaz-San Segundo, F., Weiss, M., Perez-Martin, E., Koster, M.J., Zhu, J.J., Grubman, M.J., De Los Santos, T.B. 2011. Antiviral activity of bovine type III interferon against foot-and-mouth disease virus. Virology. 413(2):283-292.
Differential gene expression in bovine cells infected with wild type and leaderless foot-and-mouth disease virus - (Peer Reviewed Journal)
Zhu, J.J., Weiss, M., Grubman, M.J., De Los Santos, T.B. 2010. Differential gene expression in bovine cells infected with wild type and leaderless foot-and-mouth disease virus. Virology. 404(1):32-40.
IgA antibody response of swine to foot-and-mouth disease virus (FMDV) infection and vaccination - (Peer Reviewed Journal)
Pacheco Tobin, J., Butler, J.E., Jew, J., Ferman, G.S., Zhu, J.J., Golde, W.T. 2010. IgA antibody response of swine to foot-and-mouth disease virus (FMDV) infection and vaccination. Clinical and Vaccine Immunology. 17(4):550-558.
IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination - (Review Article)
Pacheco Tobin, J., Butler, J.E., Jew, J., Ferman, G., Zhu, J.J., Golde, W.T. 2010. IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination. Clinical and Vaccine Immunology. p. 550-558.
Mutations in the classical swine fever virus NS4B protein affects virulence in swine - (Abstract Only)
Fernandez Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Zhiqiang, L., Risatti, G.R., Zhu, J.J., Borca, M.V. 2009. Mutations in the classical swine fever virus NS4B protein affects virulence in swine. Proceedings. P. 143.
Mutations in classical swine fever virus NS4B affect virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Lu, Z., Risatti, G.R., Zhu, J.J., Borca, M.V. 2010. Mutations in classical swine fever virus NS4B affect virulence in swine. Journal of Virology. 84(3):1536-1549.
The region between the two polyprotein initiation codons of foot-and-mouth disease virus is critical for virulence in cattle - (Peer Reviewed Journal)
Piccone, M.E., Pacheco Tobin, J., Pauszek, S.J., Borca, M.V., Rieder, A.E., Kramer Jr, E.V., Zhu, J.J., Rodriguez, L.L. 2010. The region between the two polyprotein initiation codons of foot-and-mouth disease virus is critical for virulence in cattle. Virology. 396:152-159.
Functional genomics approach to understand the role of Lpro in FMDV virulence - (Abstract Only)
De Los Santos, T.B., Grubman, M.J., Zhu, J.J. 2009. Functional genomics approach to understand the role of Lpro in FMDV virulence. American Society for Virology Meeting. Paper No. 9-21: P.262.
A Conserved Domain in the Leader Proteinase of Foot-and-Mouth Disease Virus is Required for Proper Sub-Cellular Localization and Function - (Peer Reviewed Journal)
De Los Santos, T.B., Diaz-San Segundo, F., Zhu, J.J., Koster, M.J., Dias, C., Grubman, M.J. 2008. A Conserved Domain in the Leader Proteinase of Foot-and-Mouth Disease Virus is Required for Proper Sub-Cellular Localization and Function. Journal of Virology. 83(4):1800-1810.
Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray - (Peer Reviewed Journal)
Gladue, D.P., Zhu, J.J., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Borca, M.V. 2010. Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. Virology. 138(1-2):89-96.
Cellular Changes Induced by Adenovirus Vaccine Vectors Expressing Foot-and-Mouth Disease Virus Structural and Nonstructural Proteins - (Abstract Only)
Moraes, M., O'Donnell, V.K., Burrage, T.G., Pena, L., Diaz-San Segundo, F., Koster, M.J., Zhu, J., Grubman, M.J. 2008. Cellular Changes Induced by Adenovirus Vaccine Vectors Expressing Foot-and-Mouth Disease Virus Structural and Nonstructural Proteins. European Study Group on the Molecular Biology of Picornaviruses. P 203
A Conserved Domain in the Leader Proteinase of Foot-and-Mouth Disease Virus is Required for Proper Subcellular Localization and Function - (Abstract Only)
De Los Santos, T., San Segundo-Diaz, F., Zhu, J., Koster, M.J., Grubman, M.J. 2008. A conserved domain in the leader proteinase of foot-and-mouth disease virus is required for proper subcellular localization and function. European Study Group on the Molecular Biology of Picornaviruses. P 86
DNA microarray analysis reveals crosstalk of Salmonella enterica serovar Typhimurium with AI-2 of Escherichia coli - (Abstract Only)
Jesudhasan, P.R., Cepeda, M.L., Widmer, K., Dowd, S.E., Soni, K.A., Hume, M.E., Zhu, J., Pillai, S.D. 2007. DNA microarray analysis reveals crosstalk of Salmonella enterica serovar Typhimurium with AI-2 of Escherichia coli [abstract]. In: American Society for Microbiology Cell to Cell Communication in Bacteria Abstracts, October 7-10, 2007, Austin, Texas. No. 110A.
Gene regulation of Salmonella enterica serovar Typhimurium as influenced by LuxS/AI-2 - (Abstract Only)
Jesudhasan, P.R., Cepeda, M.L., Widmer, K., Dowd, S.E., Soni, K.A., Hume, M.E., Zhu, J., Pillai, S.D. 2007. Gene regulation of Salmonella enterica serovar Typhimurium as influenced by LuxS/AI-2 [abstract]. American Society for Microbiology Cell to Cell Communication in Bacteria Abstracts, October 7-10, 2007, Austin, Texas. No. 111B.
Autoinducer-2 controls virulence of Salmonella enterica serovar Typhimurium - (Abstract Only)
Jesudhasan, P.R., Cepeda, M.L., Widmer, K., Dowd, S.E., Soni, K.A., Hume, M.E., Zhu, J., Pillai, S.D. 2007. Autoinducer-2 controls virulence of Salmonella enterica serovar Typhimurium [abstract]. American Society for Microbiology Cell to Cell Communication in Bacteria Abstracts, October 7-10, 2007, Austin, Texas. No. 108A.
Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine - (Abstract Only)
Risatti, G.R., Holinka, L.G., Fernanez-Sainz, I., Carrillo, C., Kutish, G., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V., 2007, Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine. Positive Strand RNA Virus International Conference Proceedings. P2-K27, p.116
Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Peer Reviewed Journal)
Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Kutish, G.F., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V. 2007. Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Virology. 364 (2): 371-82.
Genomics and vaccine development - (Peer Reviewed Journal)
Gay, C.G., Zuerner, R., Bannantine, J.P., Lillehoj, H.S., Zhu, J., Green, R.D., Pastoret, P.P. 2007. Genomics and vaccine development. OIE Rev. Sci Tech. 26:49-67.
GENE EXPRESSION PROFILING TO STUDY 12 CYTOKINE PRODUCTION AFTER EIMERIA INFECTION - (Abstract Only)
MAPPING QUANTITATIVE TRAIT LOCI AFFECTING DISEASE RESISTANCE TO AVIAN COCCIDIOSIS (73RD NECAB MTG, COLLEGE PARK, MD) - (Abstract Only)
MAPPING QTL LOCI AFFECTING GROWTH AND DISEASE RESISTANCE TO AVIAN COCCIDIOSIS - (Abstract Only)
ANALYSIS OF DISEASE RESISTANCE ASSOCIATED PARAMETERS IN BROILER CHICKENS - (Abstract Only)
KINETIC DIFFERENCES IN INTESTINAL AND SYSTEMIC INFERFERON-GAMMA AND ANTIGEN-SPECIFIC ANTIBODIES IN CHICKENS EXPERIMENTALLY INFECTED WITH EIMERIA MAXIMA - (Peer Reviewed Journal)
KINETIC DIFFERENCES IN INTESTINAL AND SYSTEMIC INTERFON-GAMMA AND ANTIGEN- SPECIFIC ANTIBODIES IN CHICKENS EXPERIMENTALLY INFECTED WITH EIMERIA MAXIMA - (Abstract Only)
ANALYSIS OF DISEASE RESISTANCE ASSOCIATED PARAMETERS. ANALYSIS OF DISEASE RESISTANCE ASSOCIATED PARAMETERS IN BROILER CHICKENS CHALLENGED WITH EIMERIA MAXIMA - (Peer Reviewed Journal)
MAREK'S DISEASE CHALLENGE OF AN F2 BROILER CHICKEN POPULATION - (Abstract Only)
KINETIC DIFFERENCES IN INTESTINAL AND SYSTEMIC INFERTERON-GAMMA AND ANTIGEN-SPECIFIC ANTIBODIES IN CHCKENS EXPERIMENTALLY INFECTED WITH EIMERIAMAXIMA - (Peer Reviewed Journal)
SCREENING FOR HETEROZYGOUS CHICKENS. SCREENING FOR HIGHLY HETEROZYGOUS CHICKENS IN OUTBRED COMMERCIAL BROILER LINES TO INCREASE DETECTION POWER FOR MAPPING QUANTITATIVE TRAIT LOCI - (Peer Reviewed Journal)
SCREENING FOR HIGHLY HETEROZYGOUS CHICKENS IN OUTBRED COMMERCIAL BROILER LINES TO INCREASE DETECTION POWER FOR MAPPING QUANTITATIVE TRAIT LOCI - (Abstract Only)
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