Biological Science Lab Technician
Education:
- B. S. Plant Biology, Biochemistry, University of Minnesota
- M. S. Plant Pathology, University of Minnesota
Research Interests
As a technician in the laboratory of Dr. Milton Drott, I work on the fungal pathogen Fusarium graminearum, a causal agent of Fusarium Head Blight (FHB) of cereal crops such as wheat and barley. FHB is a devasting disease worldwide caused by the fungus infecting flowering heads, resulting in yield loss not only from damaged kernels, but also due to mycotoxin contamination of the grain with trichothecene mycotoxins, including deoxynivalenol. Research in the Drott Lab includes studies of F. graminearum populations and genomes, the role of wild grasses in F. graminearum populations/ disease capabilities, and functional genomics of secondary metabolites. While I work on aspects of many of the Drott lab projects, I am most interested in functional genomics with regards to coordination of primary and secondary metabolism, and factors influencing population dynamics of F. graminearum and other Fusarium species that cause FHB.
will take you to the publication reprint.)
-(Peer Reviewed Journal)
O'Mara, S.P., Broz, K.L., Schwister, E.M., Singh, L., Dong, Y., Elmore, J.M., Kistler, H.C. 2023. The Fusarium graminearum transporters Abc1 and Abc6 are important for xenobiotic resistance, trichothecene accumulation, and virulence to wheat. Phytopathology. https://doi.org/10.1094/phyto-09-22-0345-r.
-(Peer Reviewed Journal)
O'Mara, S.P., Broz, K.L., Boenisch, M.J., Zhong, Z., Dong, Y., Kistler, H.C. 2020. The Fusarium graminearum t-SNARE Sso2 is involved in growth, defense, and DON accumulation and virulence. Molecular Plant-Microbe Interactions. 33(7):888-901. https://doi.org/10.1094/MPMI-01-20-0012-R. -(Peer Reviewed Journal)
Zhang, Y., Yang, H., Turra, D., Zhou, S., Ayhan, D.H., Delulio, G.A., Guo, L., Broz, K.L., Wiederhold, N., Coleman, J.J., O Donnell, K., Youngster, I., McAdam, A.J., Savinov, S., Shea, T., Young, S., Zeng, Q., Rep, M., Pearlman, E., Schwartz, D.C., Di Pietro, A., Kistler, H.C., Ma, L. 2020. The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes. Communications Biology. 3:50. https://doi.org/10.1038/s42003-020-0770-2. -(Peer Reviewed Journal)
Boenisch, M.J., Blum, A., Broz, K.L., Gardiner, D.M., Kistler, H.C. 2019. Nanoscale enrichment of the cytosolic enzyme trichodiene synthase near reorganized endoplasmic reticulum in Fusarium graminearum. Fungal Genetics and Biology. 124:73-77. https://doi.org/10.1016/j.fgb.2018.12.008. -(Peer Reviewed Journal)
Flynn, C.M., Broz, K.L., Jonkers, W., Schmidt-Dannert, C., Kistler, H.C. 2019. Expression of the Fusarium graminearum terpenome and involvement of the endoplasmic reticulum-derived toxisome. Fungal Genetics and Biology. 124:78-87. https://doi.org/10.1016/j.fgb.2019.01.006.
Jelinski, N.A., Broz, K.L., Jonkers, W., Ma, L., Kistler, H.C. 2017. Effector gene suites in some soil isolates of Fusarium oxysporum are not sufficient predictors of vascular wilt in tomato. Phytopathology. 107:842-851.
Boenisch, M.J., Broz, K.L., Purvine, S.O., Chrisler, W.B., Nicora, C.D., Connolly, L.R., Freitag, M., Baker, S.E., Kistler, H.C. 2017. Reorganization of the ER during mycotoxin production in Fusarium graminearum. Scientific Reports. 7:44296.
Kistler, H.C., Broz, K.L. 2015. Cellular compartmentalization of secondary metabolism. Frontiers in Microbiology. 6(68):1-11.
Varga, E., Wiesenberger, G., Hametner, C., Ward, T.J., Dong, Y., Schofbeck, D., McCormick, S.P., Broz, K.L., Stuckler, R., Kistler, H.C. 2014. Discovery and toxicity assessment of a novel type A trichothecene produced by US isolates of Fusarium graminearum [abstract]. World Mycotoxin Forum.
Varga, E., Wiesenberger, G., Hametner, C., Ward, T.J., Dong, Y., Schofbeck, D., McCormick, S.P., Broz, K.L., Stuckler, R., Schuhmacher, R., Krska, R., Kistler, H.C., Berthiller, F., Adam, G. 2015. New tricks of an old enemy: isolates of Fusarium graminearum produce a type A trichothecene mycotoxin. Environmental Microbiology. 17(8):2588-2600.
Liang, J., Xayamongkhon, H., Broz, K.L., Dong, Y., McCormick, S.P., Abramova, S., Ward, T.J., Ma, Z.H., Kistler, H.C. 2014. Temporal dynamics and population genetic structure of Fusarium graminearum in the upper Midwestern United States. Fungal Genetics and Biology. 73:83-92.
Jonkers, W., Xayamongkhon, H., Haas, M., Olivain, C., Van der Does, H., Broz, K.L., Rep, M., Alabouvette, C., Steinberg, C., Kistler, H.C. 2014. EBR1 genomic expansion and its role in virulence of Fusarium species. Environmental Microbiology. Available: http://www.ncbi.nlm.nih.gov/pubmed/24237614.
Menke, J., Weber, J., Broz, K.L., Kistler, H.C. 2013. Cellular development associated with induced secondary metabolism in the filamentous fungus Fusarium graminearum. PLoS Pathogens. 8(5):e63077.
Jonkers, W., Dong, Y., Broz, K.L., Kistler, H.C. 2012. The Wor1-like protein Fgp1 regulates pathogenicity, toxin synthesis and reproduction in the phytopathogenic fungus Fusarium graminearum. PLoS Pathogens. 8(5):e1002724.
Sarver, B.A., Ward, T.J., Gale, L.R., Broz, K.L., Kistler, H.C., Aoki, T., Nicholson, P., Carter, J., O Donnell, K. 2011. Novel fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance. Fungal Genetics and Biology. 48(12):1077-1152.
Breakspear, A., Pasquali, M., Broz, K.L., Dong, Y., Kistler, H.C. 2011. Npc1 is involved in sterol trafficking in the filamentous fungus Fusarium graminearum. Fungal Genetics Conference/Asilomar. 58(Supplement):186.
Breakspear, A., Pasquali, M., Broz, K.L., Dong, Y., Kistler, H.C. 2011. Npc1 is involved in sterol trafficking in the filamentous fungus Fusarium graminearum. Fungal Genetics and Biology. 48:725-730.
Seong, K., Pasquali, M., Zhou, X., Song, J., Hilburn, K.L., Mccormick, S.P., Dong, Y., Xu, J., Kistler, H.C. 2009. Global Gene Regulation by Fusarium Transcription Factors Tri6 and Tri10 Reveals Adaptations for Toxin Biosynthesis. Fungal Genetics Newsletter. 56:168.
Seong, K., Pasquali, M., Hilburn, K.L., Mccormick, S.P., Xu, J., Kistler, H.C. 2009. Global Gene Regulation by Fusarium Transcription Factors Tri6 and Tri10 Reveals Adaptations for Toxin Biosynthesis. Molecular Microbiology. 72:354-367.
Pasquali, M., Seong, K., Menke, J., Lysoe, E., Hilburn, K.L., Xu, J., Kistler, H.C. 2007. Spore Development and Trichothecene Mutants of Fusarium graminearum [abstract]. Proceedings of the National Fusarium Head Blight Forum. p. 32.
Cuomo, C.A., Guldener, U., Xu, J., Trail, F., Turgeon, B., Di Pietro, A., Walton, J.D., Ma, L., Baker, S.E., Rep, M., Adam, G., Antoniw, J., Baldwin, T., Calvo, S., Chang, Y., Decaprio, D., Gale, L., Gnerre, S., Goswami, R.S., Hammond-Kosack, K., Harris, L.J., Hilburn, K.L., Kennell, J., Kroken, S., Magnuson, J.K., Mannhaupt, G., Mauceli, E., Mewes, H., Mitterbauer, R., Muehlbauer, G., Munsterkotter, M., Nelson, D., O Donnell, K., Ouellet, T., Qi, W., Quesneville, H., Roncero, M.G., Seong, K., Tetko, I.V., Urban, M., Waalwijk, C., Ward, T.J., Yao, J., Birren, B.W., Kistler, H.C. 2007. The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization. Science. 317(5843):1400-1402.
