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Leslie Harden (Les)
Produce Safety and Microbiology Research
Chemist

Phone: (510) 559-5714
Fax: (510) 559-5777

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Utilising natural diversity of kinases to rationally engineer interactions with the angiosperm immune receptor ZAR1 Reprint Icon - (Peer Reviewed Journal)
Diplock, N., Baudin, M., Harden, L.A., Silva, C.J., Erickson-Beltran, M.L., Hassan, J.A., Lewis, J.D. 2023. Utilising natural diversity of kinases to rationally engineer interactions with the angiosperm immune receptor ZAR1. Plant, Cell & Environment. 46(7):2238-2254. https://doi.org/10.1111/pce.14603.
Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit Reprint Icon - (Peer Reviewed Journal)
Thilmony, R.L., Dasgupta, K., Shao, M., Harris, D., Hartman, J., Harden, L.A., Chan, R., Thomson, J.G. 2022. Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit. Frontiers in Plant Science. 13. Article 945738. https://doi.org/10.3389/fpls.2022.945738.
Characterization of a T4-like bacteriophage vB_EcoM-Sa45lw as a potential biocontrol agent for Shiga toxin-producing Escherichia coli O45 contaminated on Mung Bean seeds Reprint Icon - (Peer Reviewed Journal)
Liao, Y., Zhang, Y., Salvador, A., Harden, L.A., Wu, V.C. 2022. Characterization of a T4-like bacteriophage vB_EcoM-Sa45lw as a potential biocontrol agent for Shiga toxin-producing Escherichia coli O45 contaminated on Mung Bean seeds. Microbiology Spectrum. 10(1). Article e02220-21. https://doi.org/10.1128/spectrum.02220-21.
Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli Reprint Icon - (Peer Reviewed Journal)
Lavenburg, V., Liao, Y., Salvador, A., Hsu, A.L., Harden, L.A., Wu, V.C. 2020. Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli. Cryobiology. 96:85-91. https://doi.org/10.1016/j.cryobiol.2020.07.012.
Phage therapy for controlling Shiga toxin-producing Escherichia coli O103 using lytic bacteriophages - (Abstract Only)
Characterization of a lytic bacteriophage as an antimicrobial agent for biocontrol of Shiga toxin-producing Escherichia coli O145 strains Reprint Icon - (Peer Reviewed Journal)
Liao, Y., Salvador, A., Harden, L.A., Liu, F., Lavenburg, V.M., Li, R.W., Wu, V.C. 2019. Characterization of a lytic bacteriophage as an antimicrobial agent for biocontrol of Shiga toxin-producing Escherichia coli O145 strains. Antibiotics. 8(2):74. https://doi.org/10.3390/antibiotics8020074.
Characterization of a Lytic Bacteriophage as an Antimicrobial Agent for Biocontrol of Shiga Toxin-Producing Escherichia coli O145 Strains - (Abstract Only)
Quantitative HPLC-MS/MS analysis of toxins in soapberry (Sapindaceae) seeds: Methylenecyclopropylglycine and Hypoglycin A Reprint Icon - (Peer Reviewed Journal)
Sanford, A.A., Isenberg, S.L., Carter, M., Mojica, M.A., Matthews, T.P., Harden, L.A., Takeoka, G.R., Thomas, J.D., Pirkle, J.L., Johnson, R.C. 2018. Quantitative HPLC-MS/MS analysis of toxins in soapberry (Sapindaceae) seeds: Methylenecyclopropylglycine and Hypoglycin A. Journal of Food Chemistry. 264:449-454. https://doi.org/10.1016/j.foodchem.2018.04.093.
Identification of novel bacteriophage peptides using a combination of gene sequence LC-MS-MS analysis and BLASTP - (Abstract Only)
Escherichia coli O157:H7 converts plant-derived choline to glycine betaine for osmoprotection during pre- and post-harvest colonization of injured lettuce leaves Reprint Icon - (Peer Reviewed Journal)
Scott, R.A., Thilmony, R.L., Harden, L.A., Zhou, Y., Brandl, M. 2017. Escherichia coli O157:H7 converts plant-derived choline to glycine betaine for osmoprotection during pre- and post-harvest colonization of injured lettuce leaves. Frontiers in Microbiology. 8:2436. https://doi.org/10.3389/fmicb.2017.02436.
Association of acute toxic encephalopathy with litchi consumption in an outbreak in Muzaffarpur, India, 2014: a case-control study Reprint Icon - (Peer Reviewed Journal)
Shrivastava, A., Kumar, A., Thomas, J., Bhushan, G., Isenberg, S.L., Carter, M., Chhabra, M., Mittal, V., Khare, S., Sejvar, J., Dwivedi, M., Johnson, R., Pirkle, J.L., Yadav, R., Velayudhan, A., Pappanna, M., Singh, P., Somashekar, D., Pradhan, A., Goel, K., Pandey, R., Kumar, M., Kumar, S., Sharer, D., Hall, P., Graham, L., Matthews, T.P., Johnson, D., Schier, J., Chakrabarti, A., Perumal, S., Kumar, R., Valentin, L., Caldwell, K., Jarrett, J., Harden, L.A., Takeoka, G.R., Tong, S., Singh, R., Sing, R., Earhart, K., Dhariwal, A., Chauhan, L., Laserson, K., Venkatesh, S., Srikantiah, P. 2017. Association of acute toxic encephalopathy with litchi consumption in an outbreak in Muzaffarpur, India, 2014: a case-control study. The Lancet Global Health. 5(4):e458-e466. http://dx.doi.org/10.1016/S2214-109X(17)30035-9.
Quantification of toxins in soapberry (Sapindaceae) arils: Hypoglycin A and Methylenecyclopropylglycine - (Peer Reviewed Journal)
Isenberg, S.L., Carter, M.S., Hayes, S.R., Graham, L., Johnson, D., Matthews, T.P., Harden, L.A., Takeoka, G.R., Thomas, J.D., Pirkle, J.L., Johnson, R.C. 2016. Quantification of toxins in soapberry (Sapindaceae) arils: Hypoglycin A and Methylenecyclopropylglycine. Journal of Agricultural and Food Chemistry. 64(27):5607-5613. doi:10.1021/acs.jafc.6b02478.
A family of small cyclic amphipathic peptides (SCAmpPs) genes in citrus - (Peer Reviewed Journal)
Belknap, W.R., Mc Cue, K.F., Harden, L.A., Vensel, W.H., Bausher, M.G., Stover, E.W. 2015. A family of small cyclic amphipathic peptides (SCAmpPs) genes in citrus. Genome. 16:303-313.
Detection of methylenecyclopropylglycine (MCPG) and hypoglycin A in litchi fruit (abstract) - (Abstract Only)
Harden, L.A., Pan, J., Takeoka, G.R. 2015. Detection of methylenecyclopropylglycine (MCPG) and hypoglycin A in litchi fruit (abstract). 63rd ASMS Conference on Mass Spectrometry and Allied Topic, St. Louis, MO. May 31-June 4, 2015.
Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4 - (Peer Reviewed Journal)
Zhang, Y., Du, W., Fregevu, C., Kothary, M.H., Harden, L.A., Mchugh, T.H. 2014. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. Journal of Agricultural and Food Chemistry. 62(52):12695-12700. doi: 10.1021/jf50451021.
Antioxidant activity, phenolic and anthocyanin contents of various rhubarb (Rheum-spp.) varieties - (Peer Reviewed Journal)
Takeoka, G.R., Dao, L.T., Harden, L.A., Pantoja, A., Kuhl, J.C. 2012. Antioxidant activity, phenolic and anthocyanin contents of various rhubarb (Rheum-spp.) varieties. Journal of Food Chemistry. DOI: 10.1111/j.1365-2621.2012.03174.x.
Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California - (Peer Reviewed Journal)
Jay-Russell, M.J., Bates, A.H., Harden, L.A., Miller, W.G., Mandrell, R.E. 2012. Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California. Zoonoses and Public Health. 10.1111/j.1863-2378.2012.01465.x.
Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics - (Peer Reviewed Journal)
Fagerquist, C.K., Garbus, B.R., Miller, W.G., Williams, K.E., Yee, E., Bates, A.H., Boyle, S., Harden, L.A., Cooley, M.B., Mandrell, R.E. 2010. Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics. Analytical Chemistry. 82:2717-2725.
Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry - (Peer Reviewed Journal)
Fagerquist, C.K., Garbus, B., Williams, K., Bates, A.H., Harden, L.A. 2010. Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry. Journal of American Society for Mass Spectrometry. 21,819-832.
Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry - (Proceedings)
Fagerquist, C.K., Garbus, B.R., Williams, K.E., Bates, A.H., Boyle, S., Harden, L.A., Miller, W.G., Mandrell, R.E. 2009. Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry.IN: Proceedings of the 57th American Society of Mass Spectrometry Conference, May 31-June 4, 2009, Philadelphia, PA. DVD:A091307.8125ver.1.pdf
Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification - (Peer Reviewed Journal)
Fagerquist, C.K., Garbus, B.R., Williams, K., Bates, A.H., Boyle, S., Harden, L.A. 2009. Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification. Applied and Environmental Microbiology. 75(13):4341-4353.
Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry - (Abstract Only)
Identification of acylglycerols containing dihydroxy fatty acids in castor (Ricinus communis L.)oil by mass spectrometry - (Peer Reviewed Journal)
Lin, J.T., Arcinas, A.J., Harden, L.A. 2009. Identification of acylglycerols containing dihydroxy fatty acids in castor (Ricinus communis L.)oil by mass spectrometry. Lipids. 44:359-365.
SUB-SPECIATING CAMPYLOBACTER JEJUNI BY PROTEOMIC ANALYSIS OF ITS PROTEIN BIOMARKERS AND THEIR POST-TRANSLATIONAL MODIFICATIONS - (Peer Reviewed Journal)
Fagerquist, C.K., Bates, A.H., Heath, S., King, B.C., Garbus, B.R., Harden, L.A., Miller, W.G. Sub-speciating campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications. Journal of Proteome Research. 5:10:2527-2538
IDENTIFICATION OF (12-RICINOLEOYL-RICINOLEOYL)-DIRICINOLEOYL-GYLCEROL, AN ACYLGLYCEROL CONTAINING FOUR ACYL CHAINS, IN CASTOR BY LC-ESI-MS - (Peer Reviewed Journal)
Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-gylcerol, an acylglycerol containing four acyl chains, in castor by lc-esi-ms. Journal of Agriculture and Food Chemistry. 54(10):3498-3504.
IDENTIFICATION OF (12-RICINOLEOYL-RICINOLEOYL)-DIRICINOLEOYL-GLYCEROL IN CASTOR OIL BY ESI-MS/MS AND ITS BIOSYNTHESIS - (Abstract Only)
Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-glycerol in castor oil by esi-ms/ms and its biosynthesis. American Oil Chemists' Society Meeting.
IDENTIFICATION OF BENZALKONIUM CHLORIDE IN COMMERCIAL GRAPEFRUIT SEED EXTRACTS - (Peer Reviewed Journal)
Takeoka, G.R., Dao, L.T., Wong, R.Y., Harden, L.A. 2005. Identification of Benzalkonium Chloride in Commercial Grapefruit Seed Extracts. Journal of Agricultural and Food Chemistry. 53:7630-7636.
SPECIATION OF CAMPYLOBACTER COLI, C. JEJUNI, C. HELVETICUS, C. LARI, C. SPUTORUM, AND C. UPSALIENSIS BY MATRIX-ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROMETRY - (Peer Reviewed Journal)
Mandrell, R.E., Harden, L.A., Bates, A.H., Miller, W.G., Haddon, W.F., Fagerquist, C.K. 2005. Speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry. Applied and Environmental Microbiology. 71:10 p. 6292-6307
GENOMIC AND PROTEOMIC IDENTIFICATION OF A DNA-BINDING PROTEIN USED IN THE "FINGERPRINTING" OF CAMPYLOBACTER SPECIES AND STRAINS BY MALDI-TOF-MS PROTEIN BIOMARKER ANALYSIS - (Peer Reviewed Journal)
Fagerquist, C.K., Miller, W.G., Harden, L.A., Bates, A.H., Vensel, W.H., Wang, G., Mandrell, R.E. 2005. Genomic and proteomic identification of a dna-binding protein used in the "fingerprinting" of campylobacter species and strains by maldi-tof-ms protein biomarker analysis. Analytical Chemistry. web release 6/25/05, 77:4897-4907
DELPHINIDIN 3-O-(2-O-BETA-D-GLUCOPYRANOSYL-ALPHA-L-ARABINOPYRANOSIDE), A NOVEL ANTHOCYANIN IDENTIFIED IN BELUGA BLACK LENTILS - (Peer Reviewed Journal)
Takeoka, G.R., Dao, L.T., Tamura, H., Harden, L.A. 2005. Delphinidin 3-O-(2-O-Beta-D-Glucopyranosyl-Alpha-L-arabinopyranoside): A Novel Anthocyanin Identified in Beluga Black Lentils. Journal of Agricultural and Food Chemistry. Vol 53. p. 4932-4937.
AUTHENTICATION OF COMMERCIAL GRAPEFRUIT SEED EXTRACTS - (Abstract Only)
Takeoka, G.R., Dao, L.T., Wong, R.Y., Harden, L.A. 2005. Authentication of commercial grapefruit seed extracts. Meeting Abstract. AFGD Paper No. 50, ACS Nat'l Meeting, March 2005, San Diego, CA.
FINGERPRINTING OF CAMPYLOBACTER SPECIES AND STRAINS BY MASS SPECTROMETRIC IDENTIFICATION OF PROTEIN BIOMARKERS - (Proceedings)
Fagerquist, C.K., Harden, L.A., Miller, W.G., Fouts, D., Nelson, K., Mandrell, R.E. 2004. Fingerprinting of campylobacter species and strains by mass spectrometric identification of protein biomarkers. In: Proceedings of the 52nd American Society for Mass Spectrometry Conference and Allied Topics. May 23-27, 2004 Nashville, TN. CDROM
BIOCHEMICAL DIVERSITY AMONG THE 1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE ISOZYMES ENCODED BY THE ARABIDOPSIS GENE FAMILY - (Peer Reviewed Journal)
YAMAGAMI, T., TSUCHISAKA, A., YAMADA, K., HADDON, W.F., HARDEN, L.A., THEOLOGIS, A. Biochemical Diversity among the 1-Amino-cyclopropane-1-Carboxylate Synthase Isozymes Encoded by the Arabidopsis Gene Family. JOURNAL OF BIOLOGICAL CHEMISTRY. 2003. V. 278:49102-49112.
LIPASE-CATALYZED SYNTHESIS OF 1,2(2,3)-DIRICINOLEIN. - (Abstract Only)
TURNER, C., NGUYEN, T.T., LIN, J.T., WONG, R.Y., HARDEN, L.A., LUNDIN, R.E., MCKEON, T.A. LIPASE-CATALYZED SYNTHESIS OF 1,2(2,3)-DIRICINOLEIN.. BIOCHEMISTRY & MOLECULAR BIOLOGY OF PLANT FATTY ACIDS AND GLYCEROLIPIDS. LIPIDS, v. 38(11)2003, pp:1197-1205