Research Project: Cryopreservation of Bee Germplasm Research

Location: Insect Genetics and Biochemistry Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Fluctuating temperatures extend longevity in pupae and adult stages of the sepsid Themira biloba - (Peer Reviewed Journal)
Melicher, D.M., Bowsher, J.H., Rinehart, J.P. 2021. Fluctuating temperatures extend longevity in pupae and adult stages of the sepsid Themira biloba. Journal of Thermal Biology. 99. Article 102959. https://doi.org/10.1016/j.jtherbio.2021.102959.

Fluctuating thermal regimes extend longevity and maintain fecundity to increase colony shelf-life of Drosophila melanogaster - (Peer Reviewed Journal)
Melicher, D.M., Wilson, A.M., Yocum, G.D., Rinehart, J.P. 2021. Fluctuating thermal regimes extend longevity and maintain fecundity to increase colony shelf-life of Drosophila melanogaster. Journal of Thermal Biology. https://doi.org/10.1111/phen.12357.

Microclimate temperatures impact nesting preference in Megachile rotundata - (Peer Reviewed Journal)
Wilson, E.S., Murphy, C.E., Rinehart, J.P., Yocum, G.D., Bowsher, J.H. 2020. Microclimate temperatures impact nesting preference in Megachile rotundata. Environmental Entomology. https://doi.org/10.1093/ee/nvaa012.

A non-activating diluent to prolong in vitro viability of Apis mellifera spermatozoa: Effects on cryopreservation and on egg fertilization - (Peer Reviewed Journal)
Rajamohan, A., Danka, R.G., Hopkins, B.K., Rinehart, J.P. 2020. A non-activating diluent to prolong in vitro viability of Apis mellifera spermatozoa: Effects on cryopreservation and on egg fertilization. Journal of Cryobiology. 92:124-129. https://doi.org/10.1016/j.cryobiol.2019.11.045.

Fluctuating thermal regimes prevent chill injury but do not change patterns of oxidative stress in the alfalfa leafcutting bee, Megachile rotundata - (Peer Reviewed Journal)
Torson, A.S., Yocum, G.D., Rinehart, J.P., Nash, S.A., Bowsher, J.H. 2019. Fluctuating thermal regimes prevent chill injury but do not change patterns of oxidative stress in the alfalfa leafcutting bee, Megachile rotundata. Journal of Insect Physiology. https://doi.org/10.1016/j.jinsphys.2019.103935.

Generalized linear mixed model approach to time-to-event data with censored observations - (Proceedings)
Yeater, K.M., Yocum, G.D., Rinehart, J.P., Rajamohan, A., Bowsher, J., Greenlee, K. 2019. Generalized linear mixed model approach to time-to-event data with censored observations. In: Proceedings of the Southeast SAS Users Group Conference, October 20-22, 2019. Williamsburg, Virginia.

Thermoprofile parameters affect survival of Megachile rotundata during exposure to low-temperatures - (Peer Reviewed Journal)
Yocum, G.D., Rinehart, J.P., Rajamohan, A., Bowsher, J.H., Yeater, K.M., Greenlee, K.J. 2019. Thermoprofile parameters affect survival of Megachile rotundata during exposure to low-temperatures. Integrative & Comparative Biology. 59(4):1089-1102. https://doi.org/10.1093/icb/icz126.

Impacts of temperature stress on gene expression of the insulin pathway in overwintering Megachile rotundata - (Abstract Only)
Cambron, L.D., Yocum, G.D., Greenlee, K.J. 2019. Impacts of temperature stress on gene expression of the insulin pathway in overwintering Megachile rotundata [abstract]. Eighth International Symposium on Molecular Insect Science. July 7-10, 2019. Barcelona, Spain. Talk No. O16.

Immediate transcriptional response to a temperature pulse under a fluctuating thermal regime - (Peer Reviewed Journal)
Melicher, D.M., Torson, A.S., Anderson, T.J., Yocum, G.D., Rinehart, J.P., Bowsher, J.H. 2019. Immediate transcriptional response to a temperature pulse under a fluctuating thermal regime. Integrative & Comparative Biology. 59(2):320-337. https://doi.org/10.1093/icb/icz096.

New techniques for answering old questions: Measuring gene expression in diapausing Megachile rotundata - (Abstract Only)
Cambron, L.D., Yocum, G.D., Greenlee, K.J. 2019. New techniques for answering old questions: Measuring gene expression in diapausing Megachile rotundata [abstract]. Entomological Society of America Annual Meeting. Nov. 17-20, 2019. St. Louis, MO. Poster No. D3072.

Parental thermal history influences offspring characteristics - (Abstract Only)
Earls, K.N., Porter, M., Yocum, G.D., Greenlee, K.J. 2019. Parental thermal history influences offspring characteristics [abstract]. Entomological Society of America Annual Meeting. Nov. 17-20, 2019. St. Louis, MO. Talk No. 1019.

The influence of body size on metabolic rate in Megachile rotundata - (Abstract Only)
Grula, C., Greenlee, K.J., Rinehart, J.P., Bowsher, J.H. 2019. The influence of body size on metabolic rate in Megachile rotundata [abstract]. Entomological Society of America Annual Meeting. Nov. 17-20, 2019. St. Louis, MO. Talk No. 1024.

Effects of caste on critical PO2 and flight metabolic rate in the bumble bee, Bombus impatiens - (Abstract Only)
Walter, R., Davis, G., Rinehart, J.P., Greenlee, K.J. 2019. Effects of caste on critical PO2 and flight metabolic rate in the bumble bee, Bombus impatiens [abstract]. Entomological Society of America Annual Meeting. Nov. 17-20, 2019. St. Louis, MO. Poster No. D3102.

Let me out: Effects of offspring cell position on emergence patterns of solitary, cavity-nesting bee, Megachile rotundata - (Abstract Only)
Debardlabon, K.M., Yocum, G.D., Rinehart, J.P., Greenlee, K.J. 2019. Let me out: Effects of offspring cell position on emergence patterns of solitary, cavity-nesting bee, Megachile rotundata [abstract]. Entomological Society of America Annual Meeting. Nov. 17-20, 2019. St. Louis, MO. Poster No. D3231.

Genotoxicity assessment of agrochemicals on honey bee spermatozoa using the TUNEL assay [abstract] - (Abstract Only)
Campion, C., North, H.A., Rajamohan, A., Bowsher, J.H. 2019. Genotoxicity assessment of agrochemicals on honey bee spermatozoa using the TUNEL assay [abstract]. 2019 International Pollinator Conference, July 17-20, 2019, Davis, CA. p. 16.

Are urban pollinator plantings the bee’s knees in Fargo, ND? [abstract] - (Abstract Only)
Park, M.G., Oliveras, V.A., Rinehart, J.P., Bowsher, J.H., Greenlee, K.J. 2019. Are urban pollinator plantings the bee’s knees in Fargo, ND? [abstract]. 2019 International Pollinator Conference, July 17-20, 2019, Davis, CA. p. 33.

The impact of thiamethoxam on drone navigation and behavior [abstract] - (Abstract Only)
North, H.A., Campion, C., Rajamohan, A., Bowsher, J.H. 2019. The impact of thiamethoxam on drone navigation and behavior [abstract]. 2019 International Pollinator Conference, July 17-20, 2019, Davis, CA. p. 32.

A sweet and fat review of insulin signaling and lipid metabolism in diapausing insects - (Abstract Only)
Yocum, G.D., Cambron, L.D., Greenlee, K.J. 2019. A sweet and fat review of insulin signaling and lipid metabolism in diapausing insects [abstract]. Entomological Society of America Annual Meeting. Nov. 17-20, 2019. St. Louis, MO. Talk No. 2141.

Improved spring low-temperature storage of Megachile rotundata - (Abstract Only)
Yocum, G.D., Rajamohan, A., Bowsher, J.H., Greenlee, K.J. 2019. Improved spring low-temperature storage of Megachile rotundata [abstract]. 2019 International Pollinator Conference, July 17-20, 2019, Davis, CA. p. 42.

Road trip: How hive transportation puts stress on honey bees - (Popular Publication)
Melicher, D.M. 2019. Road trip: How hive transportation puts stress on honey bees. Entomology Today. https://entomologytoday.org/2019/04/01/road-trip-hive-transportation-stress-honey-bees/.

Effect of cryopreservation on the pre-hatching behavior in the Mexican fruit fly Anastrepha ludens Loew (Diptera, Tephritidae) - (Peer Reviewed Journal)
Rajamohan, A., Rinehart, J.P., Leopold, R.A. 2018. Effect of cryopreservation on the pre-hatching behavior in the Mexican fruit fly Anastrepha ludens Loew (Diptera, Tephritidae). Cryobiology. 80:38-42. https://doi.org/10.1016/j.cryobiol.2017.12.007.

Toxicity assessment of glyphosate on honey bee (Apis mellifera) spermatozoa - (Abstract Only)
Hoopman, A., North, H., Rajamohan, A., Bowsher, J. 2018. Toxicity assessment of glyphosate on honey bee (Apis mellifera) spermatozoa [abstract]. The Society for Integrative & Comparative Biology (SCIB) Annual Meeting 2018. January 3-7, 2018. San Francisco, CA. P2-21.

Diet quantity influence phenotypic dimorphism during honey bee (Apis mellifera) caste determination - (Abstract Only)
Bowsher, J., Slater, G., Yocum, G.D. 2017. Diet quantity influence phenotypic dimorphism during honey bee (Apis mellifera) caste determination [abstract]. Entomological Society of America Annual Meeting. Denver, CO. November 5-8, 2017. Poster SD1857.

The geometric framework for nutrition reveals interactions between protein and carbohydrate during larval growth in honey bees - (Peer Reviewed Journal)
Helm, B.R., Slater, G.P., Rajamohan, A., Yocum, G.D., Greenlee, K.J., Bowsher, J.H. 2017. The geometric framework for nutrition reveals interactions between protein and carbohydrate during larval growth in honey bees. Biology Open. 6(6):872-880. doi:10.1242/bio.022582.

Cryopreservation of embryos of the Mediterranean fruit fly Ceratitis capitata Vienna 8 Genetic Sexing Strain - (Peer Reviewed Journal)
Augustinos, A., Rajamohan, A., Kyritsis, G., Zacharopoulou, A., Haq, I., Targovska, A., Caceres, C., Bourtzis, K., Abd-Alla, A.M. 2016. Cryopreservation of embryos of the Mediterranean fruit fly Ceratitis capitata Vienna 8 Genetic Sexing Strain. PLoS One. 11(8):e0160232. Available: http://dx.doi.org/10.1371/journal.pone.0160232.

Long term storage of bee semen – A six month assessment of cryopreserved semen quality using motility as an index - (Abstract Only)