Location: Biological Control of Insects Research
2020 Annual Report
Accomplishments
1. Diet-toxicity bioassays of western and northern corn rootworms. Western and northern corn rootworms are major pests of corn production in the U.S. Corn Belt, but they are rapidly evolving resistance to all commercially available genetically modified corn varieties. The Environmental Protection Agency has mandated that registrants of Bt crops develop resistant colonies to facilitate understanding mechanisms that pests may use in surviving Bt crops and this is a major concern for the industry. Currently, diet-toxicity evaluations of each major maize seed company’s Bt toxins are conducted on proprietary artificial diets, which prevents direct comparisons of toxicity data from different seed companies. ARS researchers at Columbia, Missouri, developed advanced high throughput design and screening methods to formulate high-performing diets for use in diet-toxicity bioassays of western and northern corn rootworms. Several Industry and academic researcher groups were trained on-site to prepare and use the diets developed by ARS researchers in their own research. A Phase I Small Business Innovation Research grant was awarded to the collaborative group to further improve the packaging and shipping stability of both diets. These diets benefit researchers, industry and extension workers who must rapidly, inexpensively, easily and reproducibly assess rootworm resistance against genetically modified corn in the field, and evaluate new toxins using diet-toxicity assays.
Review Publications
Ludwick, D.C., Ericsson, A.C., Meihls, L.M., Gregory, M.L., Finke, D.L., Coudron, T.A., Hibbard, B.E., Shelby, K. 2019. Survey of bacteria associated with western corn rootworm life stages reveals no difference between insects reared in different soils. Scientific Reports. 9:15332. https://doi.org/10.1038/s41598-019-51870-x.
Yang, L., Wang, J., Jin, H., Fang, Q., Yan, Z., Lin, Z., Zou, Z., Song, Q., Stanley, D.W., Ye, G. 2019. Immune signaling pathways in the endoparasitoid, Pteromalus puparum. Archives of Insect Biochemistry and Physiology. 103(2):e21629. https://doi.org/10.1002/arch.21629.
Xu, G., Teng, Z., Gu, G., Guo, L., Wang, F., Xiao, S., Wang, J., Wang, B., Fang, Q., Wang, F., Song, Q., Stanley, D.W., Ye, G. 2019. Genomic and transcriptomic analyses of glutathione S-transferases in an endoparasitoid wasp, Pteromalus puparum. Archives of Insect Biochemistry and Physiology. 103(2):e21634. https://doi.org/10.1002/arch.21634.
Xiong, S., Yu, K., Xinhai, Y., Fang, Q., Deng, Y., Xiao, S., Yang, L., Wang, B., Wang, F., Zhichao, Y., Wang, F., Song, Q., Stanley, D.W., Ye, G. 2019. Genes acting in longevity-related pathways in the endoparasitoid, Pteromalus puparum. Archives of Insect Biochemistry and Physiology. 103(2):e21635. https://doi.org/10.1002/arch.21635.
Qi, Y., Wang, J., Xu, G., Song, Q., Stanley, D.W., Fang, Q., Ye, G. 2019. Biogenic amines biosynthetic and transduction genes in the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Archives of Insect Biochemistry and Physiology. 103(2):e21632. https://doi.org/10.1002/arch.21632.
Wang, J., Jin, H., Yang, L., Ye, X., Xiao, S., Song, Q., Stanley, D.W., Ye, G., Fang, Q. 2019. Genome-wide identification and analysis of genes encoding cuticular proteins in the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Archives of Insect Biochemistry and Physiology. 103(2):e21628. https://doi.org/10.1002/arch.21628.
Xiao, S., Wang, B., Li, K., Xiong, S., Ye, X., Wang, J., Zhang, J., Yan, Z., Wang, F., Song, Q., Stanley, D.W., Ye, G., Fang, Q. 2019. Identification and characterization of miRNAs in an endoparasitoid wasp, Pteromalus puparum. Archives of Insect Biochemistry and Physiology. 103(2):e21633. https://doi.org/10.1002/arch.21633.
Reall, T., Kraus, S., Goodman, C.L., Ringbauer Jr, J.A., Geibel, S., Stanley, D.W. 2019. Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro Cellular and Developmental Biology. 55:686-693. https://doi.org/10.1007/s11626-019-00394-9.
Zhou, K., Goodman, C.L., Ringbauer Jr, J.A., Song, Q., Beerntsen, B., Stanley, D.W. 2019. Establishment of two midgut cell lines from the fall armyworm, Spodoptera Frugiperda (Lepiodoptera: Noctuidae). In Vitro Cellular and Developmental Biology - Animal. 56:10-14. https://doi.org/10.1007/s11626-019-00420-w.
Guo, Y., Goodman, C.L., Stanley, D.W., Bonning, B.C. 2020. Cell lines for honey bee virus research. Viruses. 12(2):236. https://doi.org/10.3390/v12020236.
Zhou, K., Goodman, C.L., Ringbauer Jr, J.A., Stanley, D.W. 2020. Cell lines derived from the western corn rootworm larvae, Diabrotica virgifera virgifera (Chrysomelidae: Coleoptera). In Vitro Cellular and Developmental Biology - Animal. 56:281-285. https://doi.org/10.1007/s11626-020-00460-7.
Stanley, D.W., Kim, Y. 2019. Why most insects have very low proportions of C20 polyunsaturated fatty acids: the oxidative stress hypothesis. Archives of Insect Biochemistry and Physiology. 103(1):e21622. https://doi.org/10.1002/arch.21622.
Huynh, M.P., Hibbard, B.E., Vella, M., Lapointe, S.L., Niedz, R.P., Shelby, K., Coudron, T.A. 2019. Development of an improved and accessible diet for western corn rootworm larvae using response surface modeling. Scientific Reports. 9:16009. https://doi.org/10.1038/s41598-019-52484-z.
Ge, L., Zhou, Z., Sun, K., Huang, B., Stanley, D.W., Song, Q. 2020. The antibiotic jinggangmycin increases brown planthopper (BPH) fecundity by enhancing rice plant sugar concentrations and BPH insulin-like signaling. Chemosphere. 249:126463. https://doi.org/10.1016/j.chemosphere.2020.126463.
Yang, L., Wang, B., Qiu, L., Wan, B., Yang, Y., Liu, M., Wang, F., Fang, Q., Stanley, D.W., Ye, G. 2019. Functional characterization of a venom protein calreticulin in the Ectoparasitoid Pachycrepoideus vindemiae. Insects. 11(1):29. https://doi.org/10.3390/insects11010029.
Xu, G., Teng, Z., Gu, G., Qi, Y., Guo, L., Xiao, S., Wang, F., Fang, Q., Song, Q., Stanley, D.W., Ye, G. 2019. Genome-wide characterization and transcriptomic analyses of neuropeptides and their receptors in an endoparasitoid wasp, Pteromalus puparum. Archives of Insect Biochemistry and Physiology. 103(2):e21625. https://doi.org/10.1002/arch.21625.
Wu, J., Ge, L., Liu, F., Song, Q., Stanley, D.W. 2019. Pesticide-induced planthopper population resurgence in rice cropping systems. Annual Review of Entomology. 65:409-429. https://doi.org/10.1146/annurev-ento-011019-025215.
Ge, L., Zhou, Y., Gu, H., Wu, Q., Zhou, Z., Zheng, S., Stanley, D.W., Song, Q. 2019. Male selenoprotein F-like (SPF-L) influences female reproduction and population growth in Nilaparvata lugens (Hemiptera: Delphacidae). Frontiers in Physiology. 10:1196. https://doi.org/10.3389/fphys.2019.01196.
Pereira, A.E., Huynh, M.P., Sethi, A., Miles, A.L., French, B.W., Ellersieck, M.R., Coudron, T.A., Shelby, K., Hibbard, B.E. 2020. Baseline susceptibility of a laboratory strain of northern corn rootworm, diabrotica barberi (coleoptera: chrysomelidae) to bacillus thuringiensis traits in seedling, single plant, and diet-toxicity assays. Journal of Economic Entomology. 113(4):1955–1962. https://doi.org/10.1093/jee/toaa107.
