Research Project: Characterization and Mitigation of Bacterial Pathogens in the Fresh Produce Production and Processing Continuum

Location: Environmental Microbial & Food Safety Laboratory

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Antimicrobial effect of natural fruit extracts against Salmonella on whole and fresh-cut Cucumbers - (Peer Reviewed Journal)
Byun, S., Chen, C., Yin, H., Patel, J.R. 2022. Antimicrobial effect of natural fruit extracts against Salmonella on whole and fresh-cut Cucumbers. Journal of Food Safety. https://doi.org/10.1111/jfpp.16437.

Comparative transcriptome analysis reveals differentially expressed genes related to antimicrobial properties of lysostaphin in Staphylococcus aureus - (Peer Reviewed Journal)
Yan, X., Xie, Y., Li, C.C., Donovan, D.M., Gehring, A.G., Irwin, P.L., He, Y. 2022. Comparative transcriptome analysis reveals differentially expressed genes related to antimicrobial properties of lysostaphin in Staphylococcus aureus. Applied Microbiology and Biotechnology. 11(2):125. https://doi.org/10.3390/antibiotics11020125.

Differential survival of generic E. coli and Listeria spp. in Northeastern U.S. soils amended with dairy manure compost, poultry litter compost, and heat-treated poultry pellets and fate in raw edible radish crops - (Peer Reviewed Journal)
Limoges, M.A., Neher, D., Weicht, T.R., Millner, P.D., Sharma, M., Donnelly, C. 2021. Differential survival of generic E. coli and Listeria spp. in Northeastern U.S. soils amended with dairy manure compost, poultry litter compost, and heat-treated poultry pellets and fate in raw edible radish crops. Journal of Food Protection. https://doi.org/10.4315/JFP-21-261.

The influence of organic load and free chlorine on Salmonella cross-contamination of tomatoes in a model flume system - (Peer Reviewed Journal)
Bertoldi, B., Bardsley, C.A., Pabst, C.R., Baker, C., Gutierrez, A., De, J., Luo, Y., Schneider, K.R. 2021. The influence of organic load and free chlorine on Salmonella cross-contamination of tomatoes in a model flume system. Journal of Food Protection. https://doi.org/10.4315/jfp-21-212.

Levels of Salmonella enterica and Listeria monocytogenes in alternative irrigation water vary nased on water source on the Eastern Shore of Maryland - (Peer Reviewed Journal)
Acheamfour, C., Parveen, S., Hashem, F., Sharma, M., Gerdes, M., May, E.B., Rogers, K., Haymaker, J., Duncan, R., Foust, D., Tabodi, M., Bradshaw, R., Handy, E.T., East, C.L., Kim, S., Micallef, S., Callahan, M., Allard, S., Anderson-Coughlin, B., Craighead, S., Gartley, S., Vanore, A., Kniel, K.E., Solaiman, S., Bui, A., Craddock, H.A., Kulkarni, P., Rosenberg-Goldstein, R., Sapkota, A.R. 2021. Levels of Salmonella enterica and Listeria monocytogenes in alternative irrigation water vary nased on water source on the Eastern Shore of Maryland. Applied and Environmental Microbiology. 9:e00669-21. https://doi.org/10.1128/Spectrum.00669-21.

Nanoemulsified carvacrol as a novel washing treatment reduces Escherichia coli O157:H7 on fresh produce - (Peer Reviewed Journal)
Chen, C., Yin, H., Teng, Z., Byun, S., Guan, Y., Luo, Y., Upadhyay, A., Patel, J.R. 2021. Nanoemulsified carvacrol as a novel washing treatment reduces Escherichia coli O157:H7 on fresh produce. Journal of Food Protection. 84(12):2163-2173.

Listeria monocytogenes biofilm formation as affected by stainless steel surface topography and coating composition - (Peer Reviewed Journal)
Gu, T., Meesrisom, A., Luo, Y., Dinh, Q.N., Lin, S., Yang, M., Sharma, A., Tang, R., Zhang, J., Jia, Z., Millner, P.D., Pearlstein, A.J., Zhang, B. 2021. Listeria monocytogenes biofilm formation as affected by stainless steel surface topography and coating composition. Food Control. 130:108275. https://doi.org/10.1016/j.foodcont.2021.108275.

Enteric viruses and Pepper Mild Mottle Virus show significant correlation in select Mid-Atlantic agricultural waters - (Peer Reviewed Journal)
Anderson Coughlin, B., Craighead, S., Kelly, A., Vanore, A., Johnson, G., Jiang, C., Haymaker, J., White, C., Foust, D., Duncan, R., East, C.L., Handy, E., Bradshaw, R., Murray, R., Kulkarni, P., Solaiman, S., Betancourt, W., Gerba, C., Allard, S., Parveen, S., Hashem, F., Micallef, S., Sapkota, A., Sapkota, A., Sharma, M., Kniel, K. 2021. Enteric viruses and Pepper Mild Mottle Virus show significant correlation in select Mid-Atlantic agricultural waters. Applied and Environmental Microbiology. 87:e00211-21. https://doi.org/10.1128/AEM.00211-21.

Photo-triggered on-demand carvacrol vapor release from nano-generators for non-contact bacterial inactivation between nanomaterials and bacteria - (Peer Reviewed Journal)
Nong, W., Guan, W., Yin, Y., Lu, C., Wang, Q., Luo, Y., Zhang, B., Wu, J., Guan, Y. 2021. Photothermal metal-organic framework nano-generators for non-contact microorganism inactivation. Advanced Functional Materials. https://doi.org/10.1016/j.cej.2021.129874.

Nondestructive multiplex detection of foodborne pathogens with background microflora and symbiosis using a paper chromogenic array and advanced neural network - (Peer Reviewed Journal)
Zhen, J., Luo, Y., Wang, D., Dinh, Q., Lin, S., Sharma, A., Block, E.M., Yang, M., Gu, T., Pearlstein, A.J., Yu, H., Zhang, B. 2021. Nondestructive multiplex detection of foodborne pathogens with background microflora and symbiosis using a paper chromogenic array and advanced neural network. Biosensors and Bioelectronics. 183:113209. https://doi.org/10.1016/j.bios.2021.113209.

Microbial dynamics and the prevalence of foodborne pathogenic bacteria in sediments of Conococheague creek, Pennsylvania - (Abstract Only)

Rapid detection of Salmonella enterica in fresh produce by a novel microarray-based PathogenDx system - (Abstract Only)

Effects of hydrodynamic shear stress and equipment surface on Escherichia coli O157:H7 single- and multi-species biofilm formation - (Abstract Only)

Pre-harvest biocontrol of Listeria and Escherichia coli O157 on lettuce by lactic acid bacteria - (Abstract Only)

Characterization and mitigation of chemical oxygen demand and chlorine demand from fresh produce wash water - (Peer Reviewed Journal)
Teng, Z., Luo, Y., Zhou, B., Wang, Q., Hapeman, C.J. 2021. Characterization and mitigation of chemical oxygen demand and chlorine demand from fresh produce wash water. Food Control. 127:1008112. https://doi.org/10.1016/j.foodcont.2021.108112.

Rapid detection of Salmonella enterica in fresh produce by a novel microarray-based PathogenDx system - (Abstract Only)

Evaluation of DNA barcode abiotic surrogate as a predictor for inactivation of E. coli O157:H7 during spinach washing - (Peer Reviewed Journal)
Bolton, S., Gu, G., Gulbronson, C., Kramer, M.H., Luo, Y., Zografos, A., Nou, X. 2021. Evaluation of DNA barcode abiotic surrogate as a predictor for inactivation of E. coli O157:H7 during spinach washing. Journal of Food Science and Technology. https://doi.org/10.1016/j.lwt.2021.111321.

"Zooming" our way through virtual undergradate research training: a successful redesign of the CONSERVE summer internship program - (Peer Reviewed Journal)
Malayil, L., Negahban-Azar, M., Rosenberg Goldstein, R., Sharma, M., Gleason, J., Muise, A., Murray, R., Sapoka, A.R. 2021. "Zooming" our way through virtual undergradate research training: a successful redesign of the CONSERVE summer internship program. Journal of Microbiology and Biology Education. 22:1. https://doi.org/10.1128/jmbe.v22i1.2625.

Endophytic bacterial communities in apple leaves are minimally impacted by streptomycin use for fire blight management - (Peer Reviewed Journal)
Wallis, A., Gu, G., Ramachandran, O., Reed, E., Ottesen, A., Nou, X., Cox, K. 2021. Endophytic bacterial communities in apple leaves are minimally impacted by streptomycin use for fire blight management. Phytobiomes Journal. https://doi.org/10.1094/PBIOMES-11-20-0081-R.

Machine learning-enabled non-destructive paper chromogenic array detection of multiplexed viable pathogens on food - (Peer Reviewed Journal)
Liu, X., Yang, M., Luo, Y., Wang, S., Zhou, B., Teng, Z., Dillow, H., Gu, T., Reed, K., Sharm, A., Jia, Z., Yu, H., Zhang, B. 2021. Machine learning-enabled non-destructive paper chromogenic array detection of multiplexed viable pathogens on food. Nature Food. 2:110-117. https://www.x-mol.com/paperRedirect/1362513694001762304.

Temporal and agricultural factors influence E. coli survival in soil and transfer to cucumbers - (Peer Reviewed Journal)
Litt, P.K., Kelly, A., Omar, A., Johnson, G., Vinyard, B.T., Kniel, K.E., Sharma, M. 2021. Temporal and agricultural factors influence E. coli survival in soil and transfer to cucumbers. Applied and Environmental Microbiology. https://doi.org/10.1128/AEM.02418-20.

A novel antimicrobial technology to enhance food safety and quality of leafy vegetables using engineered water nanostructures - (Peer Reviewed Journal)
Huang, R., Vaze, N., Soorneedi, A., Moore, M., Luo, Y., Poverenov, E., Rodov, V., Demokritou, P. 2020. A novel antimicrobial technology to enhance food safety and quality of leafy vegetables using engineered water nanostructures. Environmental Science: Nano. 8:514-526. https://doi.org//10.1039/D0EN00814A.

Factors impacting water quality and microbiota during simulated dump tank wash of grape tomatoes - (Peer Reviewed Journal)
Zhou, B., Luo, Y., Teng, Z., Nou, X., Millner, P.D. 2022. Factors impacting water quality and microbiota during simulated dump tank wash of grape tomatoes. Journal of Food Protection. 84:695-703. https://doi.org/10.4315/JFP-20-343.

Alkynyl silver modified chitosan as a novel antimicrobial coating material for potential food applications - (Peer Reviewed Journal)
Mei, L., Zhang, F., Zhang, J., Li, Y., Liu, Y., Luo, Y., Wang, Q. 2020. Alkynyl silver modified chitosan as a novel antimicrobial coating material for potential food applications. Carbohydrate Polymers. 254:117416. https://doi.org/10.1016/j.carbpol.2020.117416.

Salmonella inactivation and sponge/microfiber mediated cross-contamination during papaya wash with chlorine or peracetic acid as sanitizer - (Peer Reviewed Journal)
Gu, G., Bolten, S., Mendes-Oliveira, G., Zhou, B., Teng, Z., Pearlstein, D., Luo, Y., Millner, P.D., Nou, X. 2020. Salmonella inactivation and sponge/microfiber mediated cross-contamination during papaya wash with chlorine or peracetic acid as sanitizer. Postharvest Biology and Technology. https://doi.org/10.1016/j.fm.2020.103677.

Persistence o Escherichia coli O157:H12 and Escherichia coli K12 as non-pathogenic surrogates for O157 on lettuce cultivars irrigated with alternative waters in the field - (Peer Reviewed Journal)
Yin, H., Gupta, N., Chen, C., Pradhan, A., Patel, J.R., Boomer, A.M. 2020. Persistence o Escherichia coli O157:H12 and Escherichia coli K12 as non-pathogenic surrogates for O157 on lettuce cultivars irrigated with alternative waters in the field. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2020.555459.

Zero-valent iron sand filtration can reduce human and plant pathogenic bacteria while increasing plant growth promoting bacteria in reclaimed water - (Peer Reviewed Journal)

Microbiological quality of high-demand foods from three major cities in Ecuador - (Peer Reviewed Journal)
Salazar-Llorente, E., Morales, M., Sornoza, I., Mariduena-Zavala, M., Gu, G., Nou, X., Ortiz, J., Maldonado-Alvarado, P., Manual Cevallos, J. 2020. Microbiological quality of high-demand foods from three major cities in Ecuador. International Journal of Food Microbiology. https://doi.org/10.4315/JFP-20-271.

Coronaviruses – potential human threat from foodborne transmission? - (Peer Reviewed Journal)
Thippareddi, H., Balamurugan, S., Patel, J.R., Manpreet, S., Brassard, J. 2020. Coronaviruses – potential human threat from foodborne transmission?. LWT - Food Science and Technology. https://doi.org/10.1016/j.lwt.2020.110147.

Improving temperature management and retaining quality of freshcut leafy greens by retrofitting open refrigerated retail display cases with doors - (Peer Reviewed Journal)
Brecht, J., Xie, Y., Abrahan, C., Bornhorst, E., Luo, Y., Monge-Brenes, A., Vorst, K., Brown, W. 2020. Improving temperature management and retaining quality of freshcut leafy greens by retrofitting open refrigerated retail display cases with doors. Journal of Food Engineering. https://doi.org/10.1016/j.jfoodeng.2020.110271.

Zero-valent iron-sand filtration reduces Escherichia coli in surface water and leafy green growing environments - (Peer Reviewed Journal)
Kim, S., Bradshaw, R., Kulkarni, P., Allard, S., Chiu, P.C., Sapkota, A.R., Kniel, K.E., Newell, M.J., Handy, E.T., East, C.L., Sharma, M. 2020. Zero-valent iron-sand filtration reduces Escherichia coli in surface water and leafy green growing environments. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2020.00112.

A longitudinal assessment of Escherichia coli, total coliforms, Enterococcus and Aeromonas spp. dynamics in alternative irrigation water sources: A CONSERVE study - (Peer Reviewed Journal)
Solaiman, S., Allard, S.M., Callahan, M., Jian, C., Handy, E.T., East, C.L., Haymaker, J., Bui, A., Craddock, H., Murray, R., Kulkurni, P., Anderson-Coughlin, B., Craighead, S., Gartley, S., Vanore, A., Duncan, R., Foust, D., Taabodi, M., Sapkota, A., May, E., Hashem, F., Parveen, S., Kniel, K., Sharma, M., Sapkota, A.R., Micalef, S.A. 2020. A longitudinal assessment of Escherichia coli, total coliforms, Enterococcus and Aeromonas spp. dynamics in alternative irrigation water sources: A CONSERVE study. Applied and Environmental Microbiology. https://doi.org/10.1128/AEM.00342-20.

Contamination of spinach at germination: A route to persistence and environmental reintroduction by Salmonella - (Peer Reviewed Journal)
Kumar, G., Patel, J.R., Ravishankar, S. 2020. Contamination of spinach at germination: A route to persistence and environmental reintroduction by Salmonella. International Journal of Food Microbiology. https://doi.org/10.1016/j.ijfoodmicro.2020.108646.

The presence of Cryptosporidium spp., Toxoplasma gondii, and Giardia intestinalis in potential alternative sources of agricultural water: a conserve project - (Abstract Only)

A predictive model for survival of Escherichia coli O157:H7 and generic E. coli in soil amended with animal manure - (Peer Reviewed Journal)
Pang, H., Mokhtari, A., Chen, Y., Oryang, D., Ingram, D.T., Sharma, M., Millner, P.D., Van Doren, J.M. 2020. A predictive model for survival of Escherichia coli O157:H7 and generic E. coli in soil amended with animal manure. Risk Analysis. https://doi.org/10.1111/risa.13491.

Biocontrol of Listeria on cantaloupes in the field with lactic acid bacteria - (Peer Reviewed Journal)
Yin, H., Kumar, V.N., Macarisin, D., Patel, J.R. 2020. Biocontrol of Listeria on cantaloupes in the field with lactic acid bacteria. Journal of Food Protection. https://doi.org/10.1111/jfpp.14465.

Effect of cultivars and irrigation waters on persistence of indicator bacteria on lettuce grown in high tunnel - (Peer Reviewed Journal)
Yin, H., Chen, C., Kiaranth, S., Byun, S., Mayer, C., Harriger, D., Patel, J.R., Pradhan, A. 2020. Effect of cultivars and irrigation waters on persistence of indicator bacteria on lettuce grown in high tunnel. Journal of Food Safety. https://doi.org/10.1111/jfs.12795.

Direct metatranscriptome RNA-seq and multiplex RT-PCR amplicon sequencing on Nanopore MinION - promising strategies for multiplex identification of viable pathogens in food - (Peer Reviewed Journal)
Yang, M., Cousineau, A., Liu, X., Sun, D., Li, S., Gu, T., Luo, S., Luo, Y., Xu, M., Zhang, B. 2020. Direct metatranscriptome RNA-seq and multiplex RT-PCR amplicon sequencing on Nanopore MinION - promising strategies for multiplex identification of viable pathogens in food. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.00514.

Survival of Salmonella enterica and shifts in microbial community as impacted by tomato wash water particulate size and chlorine treatment - (Peer Reviewed Journal)
Van Haute, Sam, Luo, Y., Bolten, S., Gu, G., Nou, X., Millner, P.D. 2020. Survival of Salmonella enterica and shifts in microbial community as impacted by tomato wash water particulate size and chlorine treatment. Food Control. 90:103470. https://doi.org/10.1016/j.fm.2020.103470.

Prevalence of Salmonella spp. and Listeria monocytogenes in non-traditional irrigation waters in the Mid-Atlantic United States is affected by water type, season, and recovery volume - (Peer Reviewed Journal)
Sharma, M., Handy, E.T., East, C.L., Kim, S., Jiang, C., Allard, S., Callahan, M.T., Micallef, S.A., Craighead, S., Anderson, B., Gartley, S., Vanore, A., Kniel, K., Haymaker, J.R., Duncan, R., Foust, D., White, C., Taabodi, M., Hashem, F., Parveen, S., May, E., Bui, A., Craddock, H., Kulkarni, P., Murray, R.T., Sapkota, A.R. 2020. Prevalence of Salmonella spp. and Listeria monocytogenes in non-traditional irrigation waters in the Mid-Atlantic United States is affected by water type, season, and recovery volume. PLoS One. 15(3):e0229365.

Susceptibility of foodborne pathogens to sanitizers in produce rinse water and potential induction of viable but non-culturable state - (Peer Reviewed Journal)
Gu, G., Bolten, S., Mowery, J., Mowery, J.D., Luo, Y., Nou, X. 2020. Susceptibility of foodborne pathogens to sanitizers in produce rinse water and potential induction of viable but non-culturable state. Food Control. 112:107138.

Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts - (Peer Reviewed Journal)
Yin, H., Boomer, A.M., Chen, C., Patel, J.R. 2020. Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts. International Journal of Food Science and Technology. https://doi.org/10.1111/ijfs.14520.

Genome sequences of Brevundimonas naejangsanensis strain FS1091 and Bacillus amyloliquefaciens strain FS1092 isolated from a fresh-cut produce processing plant - (Abstract Only)
Gu, G., Gonzalez-Escalona, N., Zheng, J., Bolten, S., Luo, Y., Mafiz, A., Sanchez Leon, M., Nou, X. 2020. Genome sequences of Brevundimonas naejangsanensis strain FS1091 and Bacillus amyloliquefaciens strain FS1092 isolated from a fresh-cut produce processing plant. Microbiology Resource Announcements. 9:(4). https://doi.org/10.1128/MRA.01448-19.

Influence of poultry litter amendment type and irrigation events on survival and persistence of Salmonella newport - (Peer Reviewed Journal)
Tecihmann, J., Litt, P.K., Sharma, M., Nyarko, E., Kniel, K.E. 2020. Influence of poultry litter amendment type and irrigation events on survival and persistence of Salmonella newport. Journal of Food Protection. 83: 821–828.

Microbiome convergence following sanitizer treatment and identification of sanitizer resistant species from spinach and lettuce rinse water - (Peer Reviewed Journal)
Gu, G., Ottesen, A., Bolten, S., Luo, Y., Rideout, S., Nou, X. 2019. Microbiome convergence following sanitizer treatment and identification of sanitizer resistant species from spinach and lettuce rinse water. International Journal of Food Microbiology. 318:0168. https://doi.org/10.1016/j.ijfoodmicro.2019.108458.

Salmonella inactivation and cross-contamination on cherry and grape tomatoes during washing in simulated commercial wash water - (Peer Reviewed Journal)
Bolten, S., Gu, G., Luo, Y., Van Haute, S., Zhou, B., Millner, P.D., Micallef, S.A., Nou, X. 2019. Salmonella inactivation and cross-contamination on cherry and grape tomatoes during washing in simulated commercial wash water. Food Microbiology. 87:103359. https://doi.org/10.1016/j.fm.2019.103359.

Differential effects of growth medium salinity on biofilm formation of Salmonella enterica svs. Enteritidis and Newport - (Peer Reviewed Journal)
Gu, L., Chen, Q., Guo, A., Ruan, Y., Zhang, X., Nou, X. 2020. Differential effects of growth medium salinity on biofilm formation of Salmonella enterica svs. Enteritidis and Newport. Journal of Food Protection. 83(2):196-203. https://doi.org/10.4315/0362-028X.JFP-19-418.

Comparative evaluation of irrigation waters on microbiological safety of spinach in field - (Peer Reviewed Journal)
Hsin-Bai, Y., Gu, G., Nou, X., Patel, J.R. 2019. Comparative evaluation of irrigation waters on microbiological safety of spinach in field. Journal of Applied Microbiology. https://doi.org/10.1111/jam.14436.

Inhibition of Escherichia coli O157:H7 and Salmonella enterica virulence factors by benzyl isothiocyanate - (Peer Reviewed Journal)
Patel, J.R., Yin, H., Bauchan, G.R., Mowery, J.D. 2020. Inhibition of Escherichia coli O157:H7 and Salmonella enterica virulence factors by benzyl isothiocyanate. Food Microbiology. https://doi.org/10.1016/j.fm.2019.103303.

The use of redox potential to estimate free chlorine in fresh produce washing operations: possibilities and limitations - (Peer Reviewed Journal)
Van Haute, S., Zhou, B., Luo, Y., Sampers, I., Van Haverbeke, M., Millner, P.D. 2019. The use of redox potential to estimate free chlorine in fresh produce washing operations: possibilities and limitations. Postharvest Biology and Technology. https://doi.org/10.1016/j.postharvbio.2019.110957.

Antibiofilm efficacy of Peptide 108 against listeria monocytogenes and shiga toxigenic Escherichia coli on equipment surfaces - (Peer Reviewed Journal)
Boomer, A., Hsin-Bai, Y., Patel, J.R. 2019. Antibiofilm efficacy of Peptide 108 against listeria monocytogenes and shiga toxigenic Escherichia coli on equipment surfaces. Journal of Food Protection. https://doi.org/10.4315/0362-028X.JFP-19-168.

Microbiota in ground water and alternative irrigation water, and spinach microbiota impacted by irrigation with different type of water - (Peer Reviewed Journal)
Gu, G., Yin, H., Ottesen, A., Bolten, S., Patel, J.R., Rideout, S., Nou, X. 2019. Microbiota in ground water and alternative irrigation water, and spinach microbiota impacted by irrigation with different type of water. Phytobiomes Journal. https://doi.org/10.1094/PBIOMES-09-18-0037-R .

Herbicides, antibiotics, stimulants, and disinfectants in agricultural water sources - (Peer Reviewed Journal)
Panthi, S., Sapkota, A.R., Raspannti, G., Allard, S., Bui, A., Craddock, H., Murrary, R., Zhu, L., East, C.L., Handy, E.T., Callahan, M., Haymaker, J., Kulkarni, P., Anderson, B., Craighead, S., Gartley, S., Vanore, A., Betancourt, W.Q., Duncan, R., Foust, D., Sharma, M., Micallef, S.A., Gerba, C., Parveen, S., Hashem, F., May, E., Kalmia, K., Pop, M., Ravishankar, S., Sapkota, A. 2019. Herbicides, antibiotics, stimulants, and disinfectants in agricultural water sources. Environmental Research. 174:1-8. https://doi.org/10.1016/j.envres.2019.04.011.

Nanoemulsified carvacrol as a novel washing treatment reduces Escherichia coli O157:H7 on fresh produce - (Abstract Only)
Chen, C., Hsin-Bai, Y., Byun, S., Zi, T., Luo, Y., Patel, J.R. 2019. Nanoemulsified carvacrol as a novel washing treatment reduces Escherichia coli O157:H7 on fresh produce. BARC Poster Day. 10:25.

Efficacy of natural fruit extracts in reducing Salmonella on whole cucumbers - (Abstract Only)
Byun, S., Chen, C., Hsin-Bai, Y., Patel, J.R. 2019. Efficacy of natural fruit extracts in reducing Salmonella on whole cucumbers. BARC Poster Day. 8:23.

Inactivation of Salmonella on alfalfa seeds by benzyl isothiocyanate - (Abstract Only)
Yin, H., Boomer, A., Chen, C., Patel, J.R. 2019. Inactivation of Salmonella on alfalfa seeds by benzyl isothiocyanate. BARC Poster Day. 57:72.

Biocontrol of foodborne pathogens on strawberries with lactic acid bacteria - (Abstract Only)
Boomer, A., Yin, H., Chen, C., Byun, S., Patel, J.R. 2019. Biocontrol of foodborne pathogens on strawberries with lactic acid bacteria. BARC Poster Day. 6:21.

Antimicrobial effects of natural fruit extracts against Salmonella on cucumbers - (Abstract Only)
Byun, S., Chen, C., Yin, H., Patel, J.R. 2019. Antimicrobial effects of natural fruit extracts against Salmonella on cucumbers. International Association for Food Protection. 2:194.

Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds - (Abstract Only)
Yin, H., Chen, C., Boomer, A., Patel, J.R. 2019. Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds. International Association for Food Protection. 2:197.

Impact of irrigation with wastewater and roof-harvested rainwater on the persistence of Escherichia coli surrogates on lettuce cultivars in the field - (Abstract Only)
Gupta, N., Yin, H., Boomer, A., Chen, C., Byun, S., Patel, J.R. 2019. Impact of irrigation with wastewater and roof-harvested rainwater on the persistence of Escherichia coli surrogates on lettuce cultivars in the field. International Association for Food Protection. 2:200.

Control of Listeria monocytogenes on fresh strawberries by lactic acid bacteria - (Abstract Only)
Colorado Suarez, S., Yin, H., Chen, C., Patel, J.R. 2019. Control of Listeria monocytogenes on fresh strawberries by lactic acid bacteria. International Association for Food Protection. 2:196.

Carvacrol nanoemulsion controls Escherichia coli O157:H7 on fresh produce - (Abstract Only)
Chen, C., Yin, H., Byun, S., Zi, T., Luo, Y., Patel, J.R. 2019. Carvacrol nanoemulsion controls Escherichia coli O157:H7 on fresh produce. International Association for Food Protection. 10:25.

Presence of Salmonella and Listeria monocytogenes in reclaimed and surface irrigation water sources on Maryland's eastern shore: a Conserve study - (Abstract Only)

Evaluation of zero-valent iron filtration to reduce Escherichia coli in agricultural irrigation water in laboratory and field trials - (Abstract Only)

Evaluation of Survival and Infectivity of Environmental Listeria monocytogenes Isolates in Tidal Brackish Irrigation Water - (Abstract Only)

Factors affecting Salmonella Newport survival in soil and subsequent transfer to spinach plants - (Abstract Only)

A longitudinal study using 16s rRNA gene sequence analysis of soil amended or unamended with heat-treated poultry pellets contaminated with Salmonella Newport - (Abstract Only)

Salmonella Newport in soils amended with heat-treated poultry pellets survived longer and more readily transferred to and persisted on spinach - (Peer Reviewed Journal)
Shah, M.K., Bradshaw, R., Nyarko, E., Handy, E.T., East, C.L., Millner, P.D., Bergholz, T.M., Sharma, M. 2019. Salmonella Newport in soils amended with heat-treated poultry pellets survived longer and more readily transferred to and persisted on spinach. Applied and Environmental Microbiology. 85:e02392-18. https://doi.org/10.1128/AEM.02392-18.

The efficacy of zero valent iron filtration on the reduction of Escherichia coli and Listeria monocytogenes in surface water for use in irrigation - (Peer Reviewed Journal)
Marik, C.M., Anderson, B., Gartley, S., Craighead, S., Bradshaw, R., Kulkarni, P., Sharma, M., Kniel, K.E. 2019. The efficacy of zero valent iron filtration on the reduction of Escherichia coli and Listeria monocytogenes in surface water for use in irrigation. Environmental Research. 173:33-39. https://doi.org/10.1016/j.envres.2019.02.028.

Prevalence of shiga-toxigenic and atypical enteropathogenic Escherichia coli in untreated surface water and reclaimed water in the Mid-Atlantic U.S - (Peer Reviewed Journal)
Haymaker, J., Sharma, M., Parveen, S., Hashem, F., May, E.B., Handy, E.T., White, C., East, C.L., Bradshaw, R., Micallef, S., Callahan, M., Allard, S., Anderson, B., Craighead, S., Gartley, S., Vanore, A., Kniel, K.E., Solaiman, S., Bui, A., Murray, R.T., Craddock, H.A., Kulkarni, P., Foust, D., Duncan, R., Taabodi, M., Sapkota, A.R. 2019. Prevalence of shiga-toxigenic and atypical enteropathogenic Escherichia coli in untreated surface water and reclaimed water in the Mid-Atlantic U.S. Environmental Research. 172:630-636. https://doi.org/10.1016/j.envres.2019.02.019.

Zerovalent iron-sand filtration can reduce the concentration of multiple antimicrobials in conventionally treated reclaimed wastewater - (Peer Reviewed Journal)
Kulkarni, P., Raspanti, G., Bui, A., Bradshaw, R., Kniel, K., Chiu, P., Sharma, M., Sapkota, A., Sapkota, A.R. 2019. Zerovalent iron-sand filtration can reduce the concentration of multiple antimicrobials in conventionally treated reclaimed wastewater. Environmental Research. https://doi.org/10.1016/j.envres.2019.02.012.

Impact of routine sanitation on Zone 3 microbiome in a fresh-cut produce processing facility - (Peer Reviewed Journal)
Gu, G., Ottesen, A., Bolten, S., Wang, L., Luo, Y., Rideout, S., Lyu, S., Nou, X. 2019. Impact of routine sanitation on Zone 3 microbiome in a fresh-cut produce processing facility. International Journal of Food Microbiology. 294:31-41. https://doi.org/10.1016/j.ijfoodmicro.2019.02.002 .

Survival and growth of Salmonella enterica and Listeria Monocytogenes on fresh-cut fruits and radish under different temperature abuse conditions - (Peer Reviewed Journal)
Huang, J., Zhou, B., Zheng, J., Nou, X., Luo, Y. 2019. Survival and growth of Salmonella enterica and Listeria Monocytogenes on fresh-cut fruits and radish under different temperature abuse conditions. Food Control. 100:300-304.

Role of farming practices in sustainable agriculture - (Proceedings)
Patel, J.R., Yin, H. 2019. Role of farming practices in sustainable agriculture. Food and Dairy Industry Conference Proceedings. 17-18.

Survival of Escherichia coli in manure-amended soils is affected by spatiotemporal, agricultural, and weather factors in the Mid-Atlantic United States - (Peer Reviewed Journal)
Sharma, M., Millner, P.D., Hashem, F., Vinyard, B.T., East, C.L., Handy, E.T., White, K.E., Stonebraker, R., Cotton, C.P. 2019. Survival of Escherichia coli in manure-amended soils is affected by spatiotemporal, agricultural, and weather factors in the Mid-Atlantic United States. Applied and Environmental Microbiology. 85:e02392-18. https://doi.org/10.1128/AEM.02392-18.

Incidence of fecal indicator and pathogenic bacteria in reclaimed and return flow waters in Arizona, USA - (Peer Reviewed Journal)
Zhu, L., Torres, M., Betancourt, W.Q., Sharma, M., Micallef, S.A., Gerba, C., Sapkota, A.R., Sapkota, A., Parveen, S., May, E. 2018. Incidence of fecal indicator and pathogenic bacteria in reclaimed and return flow waters in Arizona, USA. Environmental Research. 170:122-127. https://doi.org/10.1016/j.envres.2018.11.048.

Survival and growth of wild-type and rpoS-deficient Salmonella Newport strains in soil extracts amended with heat-treated poultry pellets - (Peer Reviewed Journal)
Shah, M.K., Bradshaw, R.N., Nyarko, E., Millner, P.D., Neher, D., Weicht, T., Bergholz, T.M., Sharma, M. 2019. Survival and growth of wild-type and rpoS-deficient Salmonella Newport strains in soil extracts amended with heat-treated poultry pellets. Journal of Food Protection. https://doi.org/10.4315/0362-028X.JFP-18-465.

Porous metal-organic framework (MOF) carrier for incorporation of volatile antimicrobial essential oil - (Peer Reviewed Journal)
Wu, Y., Luo, Y., Zhang, B., Zhou, B., Wang, Q. 2018. Porous metal-organic framework (MOF) carrier for incorporation of volatile antimicrobial essential oil. Food Control. 98:174-178.

Commodity specific food safety guidelines for the fresh tomato supply chain - (Trade Journal)
Mcentire, J., Gombas, D., Luo, Y., Decosta, S., Campbell, J., Dougherty, J., Danyluk, M., Roberts, M., Suslow, T., Schneider, K., Anderson, R., Cotta, S., Davis, C., Lovelace, T., Moreno, M., Valadez, A. 2019. Commodity specific food safety guidelines for the fresh tomato supply chain. United Fresh Produce Association. 3:1-51.

Assessment of current practices of organic farmers regarding biological soil amendments of animal origin in a multi-regional US study - (Peer Reviewed Journal)
Pires, A., Millner, P.D., Baron, J., Jay-Russell, M. 2018. Assessment of current practices of organic farmers regarding biological soil amendments of animal origin in a multi-regional US study. Food Protection Trends. 38:347-362.

Minimizing pathogen growth and quality deterioration of packaged leafy greens by maintaining optimum temperature in refrigerated display cases with doors - (Peer Reviewed Journal)
De Frias, A.J., Luo, Y., Zhou, B., Turner, E.R., Millner, P.D., Nou, X. 2018. Minimizing pathogen growth and quality deterioration of packaged leafy greens by maintaining optimum temperature in refrigerated display cases with doors. Food Control. 92:488-495.

Are cruciferous vegetables the key to combat foodborne pathogens infections? - (Abstract Only)
Yin, H., Patel, J.R., Mowery, J.D., Bauchan, G.R. 2018. Are cruciferous vegetables the key to combat foodborne pathogens infections?. BARC Poster Day. 53:66.

Antibiofilm efficacy of peptide against listeria monocytogenes and shiga toxigenic escherichia coli on equipment eurfaces - (Abstract Only)
Boomer, A., Yin, H., Patel, J.R. 2018. Antibiofilm efficacy of peptide against listeria monocytogenes and shiga toxigenic escherichia coli on equipment eurfaces. BARC Poster Day. p.6.

Presence of bacterial pathogens in fresh produce from local retail markets in Maryland region - (Abstract Only)
Vaidehi, B., Patel, J.R. 2018. Presence of bacterial pathogens in fresh produce from local retail markets in Maryland region. International Association for Food Protection. p. 160.

Impact of wastewater and roof-harvest water irrigation on microbial quality of spinach - (Abstract Only)
Yin, H., Gu, G., Nou, X., Patel, J.R. 2018. Impact of wastewater and roof-harvest water irrigation on microbial quality of spinach. International Association for Food Protection. p.162.

Microbiological quality of spinach irrigated with reclaimed wastewater and roof-harvest water - (Peer Reviewed Journal)
Hsin-Bai, Y., Nou, X., Patel, J.R., Gu, G. 2018. Microbiological quality of spinach irrigated with reclaimed wastewater and roof-harvest water. Journal of Applied Microbiology. 125:133-141. https://doi.org/10.1111/jam.13746.

Can UV absorbance rapidly estimate the chlorine demand in wash water during fresh-cut produce washing processes? - (Peer Reviewed Journal)
Van Haute, S., Luo, Y., Sampers, I., Mei, L., Teng, Z., Zhou, B., Bornhorst, E., Wang, Q., Millner, P.D. 2018. Can UV absorbance rapidly estimate the chlorine demand in wash water during fresh-cut produce washing processes? Postharvest Biology and Technology. 142:19-27. https://doi.org/10.1016/j.postharvbio.2018.02.002.

Inactivation of Escherichia coli O157:H7 and Salmonella on fresh herbs by plant essential oils - (Peer Reviewed Journal)
Patel, J.R., Keelara, S., Green, J.A. 2018. Inactivation of Escherichia coli O157:H7 and Salmonella on fresh herbs by plant essential oils. Foodborne Pathogens and Disease. 15(6):1-7. https://doi.org/10.1089/fpd.2017.2377.

Comparison of methods to determine the microbial quality of alternative irrigation waters - (Peer Reviewed Journal)
Hsin-Bai, Y., Patel, J.R. 2018. Comparison of methods to determine the microbial quality of alternative irrigation waters. Agricultural Water Management. 201:38-45. https://doi.org/10.1016/j.agwat.2018.01.012.

Shifts in spinach microbial communities after chlorine washing and storage at compliant and abusive temperatures - (Peer Reviewed Journal)
Gu, G., Ottesen, A., Bolten, S.J., Ramachandran, P., Reed, E., Rideout, S., Luo, Y., Patel, J.R., Brown, E., Nou, X. 2018. Shifts in spinach microbial communities after chlorine washing and storage at compliant and abusive temperatures. Food Microbiology. 73:73-84. https://doi.org/10.1016/j.fm.2018.01.002.

Listeria monocytogenes persistence and transfer to cantaloupes in the packing environment is affected by equipment surface type and cleanliness - (Peer Reviewed Journal)
Nyarko, E., Kniel, K.E., Zhou, B., Millner, P.D., Luo, Y., Handy, E.T., East, C.L., Sharma, M. 2018. Listeria monocytogenes persistence and transfer to cantaloupes in the packing environment is affected by equipment surface type and cleanliness. Food Control. 85:177-185.

Visualizing pathogen internalization pathways in fresh tomatoes using MicroCT and confocal laser scanning microscopy - (Peer Reviewed Journal)
Zhou, B., Luo, Y., Bauchan, G.R., Feng, H., Stommel, J.R. 2017. Visualizing pathogen internalization pathways in fresh tomatoes using MicroCT and confocal laser scanning microscopy. Food Control. 85:276-282.

Association between bacterial survival and free chlorine concentration during commercial fresh-cut produce wash operation - (Peer Reviewed Journal)
Luo, Y., Zhou, B., Van Haute, S., Nou, X., Zhang, B., Teng, Z., Turner, E.R., Wang, Q., Millner, P.D. 2017. Association between bacterial survival and free chlorine concentration during commercial fresh-cut produce wash operation. Food Microbiology. 70:120-128.

Enhanced biofilm formation in dual-species culture of Listeria monocytogenes and Ralstonia insidiosa - (Peer Reviewed Journal)
Xu, Y., Nagy, A., Bauchan, G.R., Xia, X., Nou, X. 2017. Enhanced biofilm formation in dual-species culture of Listeria monocytogenes and Ralstonia insidiosa. AIMS Environmental Science. 3(4):774-783.

Internalization of Listeria monocytogenes in cantaloupes during dump tank washing and hydrocooling - (Peer Reviewed Journal)
Macarisin, D., Wooten, A., De Jesus, A., Hur, M., Bae, S., Patel, J.R., Evans, P., Brown, E., Hammack, T., Chen, Y. 2017. Internalization of Listeria monocytogenes in cantaloupes during dump tank washing and hydrocooling. International Journal of Food Microbiology. 527:165-175.

Assessment of microbial quality of reclaimed water, roof-harvest water, and creek water for irrigation - (Abstract Only)
Yin, H., Green, J.A., Patel, J.R. 2017. Assessment of microbial quality of reclaimed water, roof-harvest water, and creek water for irrigation. BARC Poster Day. p.67. April 26, 2017.

Water in agriculture - (Peer Reviewed Journal)
Markland, S.M., Ingram, D.T., Kniel, K.E., Sharma, M. 2017. Water in agriculture. Microbiology Spectrum. (5)3.

Assessment of microbial quality of reclaimed water, roof-harvest water, and creek water for irrigation - (Abstract Only)
Yin, H., Green, J.A., Patel, J.R. 2017. Assessment of microbial quality of reclaimed water, roof-harvest water, and creek water for irrigation. International Food Technology Meeting Abstracts. p.06-53. June 24-28, 2017.

Practices and conditions which promote persistence of Listeria monocytogenes on equipment surfaces and transfer to cantaloupes in the packing environment - (Abstract Only)

Prevalence of E. coli, Salmonella spp. and L. monocytogenes in non-traditional irrigation waters in the Mid-Atlantic U.S.: a conserve project - (Abstract Only)

Assessment of indicator bacteria and Aeromonas spp. in surface and nontraditional irrigation water: a conserve study - (Abstract Only)

Zero-valent iron-biosand filtration is capable of reducing antimicrobials and generic E. coli concentrations in unbuffered conventionally-treated reclaimed water: a CONSERVE project - (Abstract Only)

Generic E. coli levels in surface and nontraditional irrigation water in the mid Atlantic in relation to FSMA water quality standards: A CONSERVE study - (Abstract Only)

The effect of soil management on the persistence of E. coli and Listeria spp. in manure- amended soils in the Northeast United States - (Abstract Only)

Screening of non-traditional irrigation water sources for Shiga toxin-producing Escherichia coli in the mid-Atlantic region of the United States: a conserve study - (Abstract Only)

Influence of amendment type on persistence of Salmonella Newport in soil - (Abstract Only)

Persistence and transmission of Escherichia coli and Salmonella in a watermelon field ammended with poultry itter: year two - (Abstract Only)

Evaluation of Listeria monocytogenes survival and infectivity in non-traditional agricultural waters - (Abstract Only)

Survival of generic E. coli and Listeria spp. populations in dairy compost- and poultry litter compost-amended soils in the Northeastern United States - (Abstract Only)

A multi-site, multi-year examination of factors affecting the persistence of attenuated O157- and non-pathogenic Escherichia coli in manure-amended soils in the mid-Atlantic United States - (Abstract Only)

Persistence of non-O157 Shiga Toxin-producing Escherichia coli on fresh produce surfaces - (Abstract Only)
Green, J.A., Patel, J.R. 2017. Persistence of non-O157 Shiga Toxin-producing Escherichia coli on fresh produce surfaces. International Association for Food Protection. p.03-67. July 9-12, 2017.

Investigation on chlorine-based sanitization under stabilized conditions in the presence of organic load - (Peer Reviewed Journal)
Teng, Z., Luo, Y., Alborzi, S., Zhou, B., Chen, L., Zhang, J., Zhang, B., Millner, P.D., Wang, Q. 2017. Investigation on chlorine-based sanitization under stabilized conditions in the presence of organic load. International Journal of Food Microbiology. 67:150-157.

Antimicrobials for foodborne pathogens inactivation on cantaloupe - (Abstract Only)
Patel, J.R., Abhinav, U., Kumar, V. 2017. Antimicrobials for foodborne pathogens inactivation on cantaloupe. ARS Food Safety and Inspection Service Research Workshop. Secrion P, p.4.

Dual effectiveness of ascorbic acid and ethanol combined treatment to inhibit browning and inactivate pathogens on fresh-cut apples - (Peer Reviewed Journal)
Yan, S., Luo, Y., Zhou, B., Ingram, D. 2017. Dual effectiveness of ascorbic acid and ethanol combined treatment to inhibit browning and inactivate pathogens on fresh-cut apples. LWT - Food Science and Technology. 80:311-320.

Soil amendments and Listeria monocytogenes and Salmonella newport survival on cucurbits - (Abstract Only)

Survival of Salmonella Newport on whole and fresh-cut cucumbers treated with lytic bacteriophages - (Peer Reviewed Journal)
Sharma, M., Dashiell, G., Handy, E.T., East, C.L., Reynnells, R., White, C., Nyarko, E., Hashem, F., Millner, P.D. 2017. Survival of Salmonella Newport on whole and fresh-cut cucumbers treated with lytic bacteriophages. Journal of Food Protection. 80:668-673.

Microbiological quality of fresh nopal juice - (Peer Reviewed Journal)
Hernandez-Anguiano, A.M., Salgado, P.L., Eslava-Campos, C.A., Hernandez, M.V., Patel, J.R. 2016. Microbiological quality of fresh nopal juice. Microorganisms. 4(46):1-11.

Novel antimicrobials to control foodborne pathogens on cantaloupe - (Proceedings)
Patel, J.R., Upadhyay, A., Venkitanarayanan, K. 2016. In: Novel antimicrobials to control foodborne pathogens on cantaloupe. United States-Japan Cooperative Program in Natural Resources Conference Proceedings, November 7, 2016. p.29-30.

Guidelines to validate control of cross-contamination during washing of fresh-cut leafy vegetables - (Peer Reviewed Journal)
Gombas, D., Luo, Y., Brennan, J., Shergill, G., Petran, R., Walsh, R., Hau, H., Khurana, K., Zomorodi, B., Rosen, J., Varley, R., Deng, K. 2017. Guidelines to validate control of cross-contamination during washing of fresh-cut leafy vegetables. Journal of Food Protection. 80(2):312-330.

Nanotechnology applications to improve food safety - (Book / Chapter)
Zhang, B., Luo, Y., Chen, H. 2017. Nanotechnology applications to improve food safety. Food Safety in China: Technology, Management and Regulation. https://doi.org/10.1002/9781119238102.ch36.

Survival and growth of Listeria monocytogenes on fresh-cut ‘Athena’ and ‘Rocky Ford’ cantaloupes during storage at 4 and 10°C - (Peer Reviewed Journal)
Nyarko, E., Kniel, K., Reynnells, R., East, C.L., Handy, E.T., Luo, Y., Millner, P.D., Sharma, M. 2016. Survival and growth of Listeria monocytogenes on fresh-cut ‘Athena’ and ‘Rocky Ford’ cantaloupes during storage at 4 and 10°C. Foodborne Pathogens and Disease. 13:587-591.