Research Project: Nutritional Role of Phytochemicals

Location: Children's Nutrition Research Center

Publications (Clicking on the reprint icon will take you to the publication reprint.)

A comprehensive UHPLC-MS/MS and extraction method for spinach (Spinacia oleracea) flavonoids - (Rapid Release Publication)
Dzakovich, M.P., Le, E.A., Tak, A.L., Chacko, S.J. 2024. A comprehensive UHPLC-MS/MS and extraction method for spinach (Spinacia oleracea) flavonoids. bioRxiv. https://doi.org/10.1101/2024.09.13.612955.

Slant board mounting fixture for seed germination - (Database / Dataset)
Chandramouli, S.K., Dzakovich, M.P. 2024. Slant board mounting fixture for seed germination. NIH 3D. https://doi.org/10.60705/3dpx/21396.1.

Discovery of steroidal alkaloid metabolites and their accumulation in pigs after short-term tomato consumption - (Peer Reviewed Journal)
Sholola, M.J., Goggans, M.L., Dzakovich, M.P., Francis, D.M., Jacobi, S.K., Cooperstone, J.L. 2024. Discovery of steroidal alkaloid metabolites and their accumulation in pigs after short-term tomato consumption. Food Chemistry. https://doi.org/10.1016/j.foodchem.2024.141346.

Development of a novel amplifiable system to quantify hydrogen peroxide in living cells - (Peer Reviewed Journal)
Wang, L., Lin, H., Yang, B., Jiang, X., Chen, J., Chowdhury, S.R., Cheng, N., Nakata, P.A., Lonard, D.M., Wang, M.C., Wang, J. 2024. Development of a novel amplifiable system to quantify hydrogen peroxide in living cells. Journal of the American Chemical Society. 146:22396-22404. https://doi.org/10.1021/jacs.4c05366.

The relative validity of nutrition assessment methods for estimating infant carotenoid intake differs by assessment tool, nutrient database, and milk carotenoid adjustment method - (Peer Reviewed Journal)
Juneja, S., Chang, J., Nguyen, T., Castaneda, R., O'Connor, T., Musaad, S., Moran, N.E. 2024. The relative validity of nutrition assessment methods for estimating infant carotenoid intake differs by assessment tool, nutrient database, and milk carotenoid adjustment method. Nutrition Research. https://doi.org/10.1016/j.nutres.2024.06.003.

Stackable 50 mL tube rack - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2024. Stackable 50 mL tube rack. NIH 3D. https://doi.org/10.60705/3DPX/21168.1.

Stackable 15 mL tube rack - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2024. Stackable 15 mL tube rack. NIH 3D. https://doi.org/10.60705/3dpx/21167.1.

Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway - (Peer Reviewed Journal)
Lan, H., Ran, J., Wang, W., Zhang, L., Wu, N., Zhao, Y., Huang, M., Ni, M., Liu, F., Cheng, N., Nakata, P.A., Pan, J., Whitham, S.A., Baker, B.J., Liu, J. 2024. Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway. Plant Communications. 5(8). Article 100937. https://doi.org/10.1016/j.xplc.2024.100937.

Constructing an environmental control module with autonomous CO2 and pressure control - (Abstract Only)

The Children's Nutrition Research Center: Leveraging controlled environment agriculture to improve pediatric health - (Abstract Only)

Nothing to be salty about: Nutrient and salinity stress improves carotenoid bioaccessibility from spinach - (Abstract Only)

Disruption of the Arabidopsis Acyl-Activating Enzyme 3 impairs seed coat mucilage accumulation and seed germination - (Peer Reviewed Journal)
Cheng, N., Nakata, P.A. 2024. Disruption of the Arabidopsis Acyl-Activating Enzyme 3 impairs seed coat mucilage accumulation and seed germination. International Journal of Molecular Sciences. 25(2). Article 1149. https://doi.org/10.3390/ijms25021149.

Exploring the role of genetic diversity on spinach flavonoid bioaccessibility - (Abstract Only)

Transcriptomics and metabolomics reveal tomato consumption alters hepatic xenobiotic metabolism and induces steroidal alkaloid metabolite accumulation in mice - (Peer Reviewed Journal)
Dzakovich, M.P., Goggans, M.L., Thomas-Ahner, J., Moran, N.E., Clinton, S.K., Francis, D.M., Cooperstone, J.L. 2024. Transcriptomics and metabolomics reveal tomato consumption alters hepatic xenobiotic metabolism and induces steroidal alkaloid metabolite accumulation in mice. Molecular Nutrition and Food Research. https://doi.org/10.1002/mnfr.202300239.

Skin carotenoids measured by reflection spectroscopy correlate with dietary carotenoid intake in racially and ethnically diverse US toddlers from Houston, Texas - (Peer Reviewed Journal)
Varghese, V., Cepni, A.B., Chang, J., Kim, H., Moran, N.E., Ledoux, T.A. 2023. Skin carotenoids measured by reflection spectroscopy correlate with dietary carotenoid intake in racially and ethnically diverse US toddlers from Houston, Texas. Journal of the Academy of Nutrition and Dietetics. 124(5):628-635. https://doi.org/10.1016/j.jand.2023.10.015.

Sequential removal of cation/H+ exchangers reveals their additive role in elemental distribution, calcium depletion and anoxia tolerance - (Peer Reviewed Journal)
Mathew, I.E., Rhein, H.S., Yang, J., Gradogna, A., Carpaneto, A., Guo, Q., Tappero, R., Scholz-Starke, J., Barkla, B., Hirschi, K.D., Punshon, T. 2023. Sequential removal of cation/H+ exchangers reveals their additive role in elemental distribution, calcium depletion and anoxia tolerance. Plant Cell and Environment. 47(2):557-573. https://doi.org/10.1111/pce.14756.

Nothing to be salty about: Nutrient and salinity stress improves carotenoid bioaccessibility from spinach - (Abstract Only)

Examining potential modifiers of human skin and plasma carotenoid responses in a randomized trial of a carotenoid-containing juice intervention - (Peer Reviewed Journal)
Jilcott Pitts, S.B., Wu, Q., Moran, N.E., Laska, M.N., Harnack, L. 2023. Examining potential modifiers of human skin and plasma carotenoid responses in a randomized trial of a carotenoid-containing juice intervention. Journal of Nutrition. 153(11):3287-3294. https://doi.org/10.1016/j.tjnut.2023.09.014.

Preterm pigs fed donor human milk have greater liver beta-carotene concentrations than pigs fed infant formula - (Peer Reviewed Journal)
Moran, N.E., Wade, J., Stroh, R., Stoll, B., Guthrie, G., Hair, A.B., Burrin, D.G. 2023. Preterm pigs fed donor human milk have greater liver beta-carotene concentrations than pigs fed infant formula. Journal of Nutrition. https://doi.org/10.1016/j.tjnut.2023.08.026.

6 x 4 PoxyGrid 15 mL conical tube metal rack adapter - orbital shaker to vortexer conversion - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2023. 6 x 4 PoxyGrid 15 mL conical tube metal rack adapter - orbital shaker to vortexer conversion. Government Publication/Report. https://3d.nih.gov/entries/3DPX-020366.

10 x 8 PoxyGrid 15 mL shaker lid - orbital shaker to vortexer conversion - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2023. 10 x 8 PoxyGrid 15 mL shaker lid - orbital shaker to vortexer conversion. Government Publication/Report. https://3d.nih.gov/entries/3DPX-020365.

Adjustable shaking incubator tube rack holder - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2023. Adjustable shaking incubator tube rack holder. Government Publication/Report. https://3d.nih.gov/entries/3DPX-020364.

One gallon pot six plant punch - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2023. One gallon pot six plant punch. Government Publication/Report. https://3d.nih.gov/entries/3DPX-020363.

Multipurpose universal laboratory tube rack - (Database / Dataset)
Chlouber, B.D., Dzakovich, M.P. 2023. Multipurpose universal laboratory tube rack. Government Publication/Report. https://3d.nih.gov/entries/3DPX-020367.

Trait stacking simultaneously enhances provitamin A carotenoid and mineral bioaccessibility in biofortified sorghum bicolor - (Peer Reviewed Journal)
Dzakovich, M.P., Debelo, H., Albertsen, M., Che, P., Jones, T., Simon, M., Zhao, Z., Glassman, K., Ferruzzi, M. 2023. Trait stacking simultaneously enhances provitamin A carotenoid and mineral bioaccessibility in biofortified sorghum bicolor. Food & Function. https://doi.org/10.1039/D2FO03606A.

Orchestration of miRNA patterns by testosterone and dietary tomato carotenoids during early prostate carcinogenesis in TRAMP mice - (Peer Reviewed Journal)
Wan, L., Thomas-Ahner, J.M., Pearl, D.K., Erdman, J.W., Moran, N.E., Clinton, S.K. 2023. Orchestration of miRNA patterns by testosterone and dietary tomato carotenoids during early prostate carcinogenesis in TRAMP mice. Journal of Nutrition. 153(7):1877-1888. https://doi.org/10.1016/j.tjnut.2023.05.015.

Transcriptomics and metabolomics reveal tomato consumption alters hepatic xenobiotic metabolism and induces steroidal alkaloid metabolite accumulation in mice - (Pre-print Publication)
Dzakovich, M.P., Goggans, M.L., Thomas-Ahner, J., Moran, N.E., Clinton, S.K., Francis, D.M., Cooperstone, J.L. 2023. Transcriptomics and metabolomics reveal tomato consumption alters hepatic xenobiotic metabolism and induces steroidal alkaloid metabolite accumulation in mice. bioRxiv. https://doi.org/10.1101/2023.04.18.536606.

Soybean GmSAUL1, a bona fide U-Box E3 ligase, negatively regulates immunity likely through repressing the activation of GmMPK3 - (Peer Reviewed Journal)
Li, J., Ye, M., Wang, C., Ma, X., Wu, N., Zohng, C., Zhang, Y., Cheng, N., Nakata, P.A., Zeng, L., Liu, J. 2023. Soybean GmSAUL1, a bona fide U-Box E3 ligase, negatively regulates immunity likely through repressing the activation of GmMPK3. International Journal of Molecular Sciences. 24. Article 6240. https://doi.org/10.3390/ijms24076240.

Reflection spectroscopy-assessed skin carotenoids are sensitive to change in carotenoid intake in a six-week randomized controlled feeding trial in a racially/ethnically diverse sample - (Peer Reviewed Journal)
Jillcot Pitts, S., Moran, N.E., Laska, M.N., Wu, Q., Harnack, L., Moe, S., Carr-Manthe, P., Gates, E., Chang, J., Zaidi, Y., Gelineau, A., Berg, L., Craft, N.E. 2023. Reflection spectroscopy-assessed skin carotenoids are sensitive to change in carotenoid intake in a six-week randomized controlled feeding trial in a racially/ethnically diverse sample. Journal of Nutrition. 153:1133-1142. https://doi.org/10.1016/j.tjnut.2023.02.017.

Instead of calories, should we be counting our consumption of exosomes and microRNAs? - (Review Article)
Hirschi, K.D., Rhein, H.S., Nalliah, V. 2023. Instead of calories, should we be counting our consumption of exosomes and microRNAs? MicroRNA. https://doi.org/10.2174/2211536612666230331083455.

Liver specific disruption of Glutaredoxin 3 leads to iron accumulation and impaired cellular iron homeostasis - (Peer Reviewed Journal)
Cheng, N., Donelson, J., Breton, G., Nakata, P.A. 2023. Liver specific disruption of Glutaredoxin 3 leads to iron accumulation and impaired cellular iron homeostasis. Biochemical and Biophysical Research Communications. 649:39-46. https://doi.org/10.1016/j.bbrc.2023.01.095.

Generating reproducing anoxia conditions for plant phenotyping - (Peer Reviewed Journal)
Mathew, I.E., Rhein, H.S., Green, A.J., Hirschi, K.D. 2023. Generating reproducing anoxia conditions for plant phenotyping. Bio-protocol. 13(3). https://doi.org/10.21769/BioProtoc.4603.

Natural history of TANGO2 deficiency disorder: Baseline assessment of 73 patients - (Peer Reviewed Journal)
Miyake, C.Y., Lay, E.J., Soler-Alfonso, C., Glinton, K.E., Houck, K.M., Tosur, M., Moran, N.E., Stephens, S.B., Scaglia, F., Howard, T.S., Kim, J.J., Pham, T., Valdres, S.O., Li, N., Murali, C.N., Zhang, L., Kava, M., Yim, D., Beach, C., Webster, G., Liberman, L., Janson, C.M., Kannankeril, P.J., Baxter, S., Singer-Berk, M., Wood, J., Mackenzie, S.J., Sacher, M., Ghaloul-Gonzalez, L., Pedroza, C., Morris, S.A., Ehsan, S.A., Azamian, M.S., Lalani, S.R. 2022. Natural history of TANGO2 deficiency disorder: Baseline assessment of 73 patients. Genetics in Medicine. 25(4). Article 100352. https://doi.org/10.1016/j.gim.2022.11.020.

Crucial role of Arabidopsis glutaredoxin S17 in heat stress response revealed by transcriptome analysis - (Peer Reviewed Journal)
Rao, X., Cheng, N., Mathew, I.E., Hirschi, K.D., Nakata, P.A. 2022. Crucial role of Arabidopsis glutaredoxin S17 in heat stress response revealed by transcriptome analysis. Functional Plant Biology. 50(1):58-70. https://doi.org/10.1071/FP22002.

Non-invasive, reflection spectroscopy measurement of skin carotenoid score in infants is feasible and reliable - (Peer Reviewed Journal)
Moran, N.E., Chang, J., Stroh, R., Zaidi, Y., Hason, N., Musaad, S., O'Connor, T. 2022. Non-invasive, reflection spectroscopy measurement of skin carotenoid score in infants is feasible and reliable. Journal of Nutrition. https://doi.org/10.1093/jn/nxac182 .

Trait stacking simultaneously enhances provitamin a carotenoid and mineral bioaccessibility in biofortified sorghum bicolor - (Other)
Dzakovich, M.P., Debelo, H., Albertsen, M., Che, P., Jones, T., Simon, M., Zhao, Z., Glassman, K., Ferruzzi, M. 2022. Trait stacking simultaneously enhances provitamin a carotenoid and mineral bioaccessibility in biofortified sorghum bicolor. bioRxiv. https://doi.org/10.1101/2022.08.03.501587.

The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses - (Peer Reviewed Journal)
Yang, J., Mathew, I., Rhein, H., Barker, R., Guo, Q., Brunello, L., Loreti, E., Barkla, B., Gilroy, S., Perata, P., Hirschi, K.D. 2022. The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses. Plant Physiology. 190(4):2617-2636. https://doi.org/10.1093/plphys/kiac375.

A conserved oxalyl-Coenzyme A decarboxylase in oxalate catabolism - (Peer Reviewed Journal)
Cheng, N., Paris, V., Rao, X., Wang, X., Nakata, P.A. 2022. A conserved oxalyl-coenzyme A decarboxylase in oxalate catabolism. Plant Signaling and Behavior. 17:1. https://doi.org/10.1080/15592324.2022.2062555.

Tomatoes, lycopene, and prostate cancer: What have we learned from experimental models? - (Review Article)
Moran, N.E., Thomas-Ahner, J.M., Wan, L., Zuniga, K.E., Erdman, J.W., Clinton, S.K. 2022. Tomatoes, lycopene, and prostate cancer: What have we learned from experimental models? Journal of Nutrition. https://doi.org/10.1093/jn/nxac066.

Systematic review of carotenoid concentrations in human milk and infant blood - (Review Article)
Zaidi, Y., Moran, N.E., Stroh, R. 2022. Systematic review of carotenoid concentrations in human milk and infant blood. Nutrition Reviews. https://doi.org/10.1093/nutrit/nuac018.

Assessment of dietary carotenoid intake and biologic measurement of exposure in humans - (Book / Chapter)
Grainger, E.M., Webb, M.Z., Simpson, C.M., Chitchumroonchokchai, C., Riedl, K., Moran, N.E., Clinton, S.K. 2022. Assessment of dietary carotenoid intake and biologic measurement of exposure in humans. In: Wurtzel, E.T. editor. Methods in Enzymology. 1st edition. Cambridge, MA: Academic Press. p. 255-295. https://doi.org/10.1016/bs.mie.2022.05.007.

Beta-carotene oxygenase 2 genotype modulates the impact of dietary lycopene on gene expression during early TRAMP prostate carcinogenesis - (Peer Reviewed Journal)
Moran, N.E., Thomas-Ahner, J.M., Smith, J.W., Silva, C., Hason, N.A., Erdman, J.W., Clinton, S.K. 2021. Beta-carotene oxygenase 2 genotype modulates the impact of dietary lycopene on gene expression during early TRAMP prostate carcinogenesis. Journal of Nutrition. 152(4):950-960. https://doi.org/10.1093/jn/nxab445.

Steroidal alkaloid biosynthesis is coordinately regulated and differs among tomatoes in the red-fruited clade - (Peer Reviewed Journal)
Dzakovich, M.P., Francis, D.M., Cooperstone, J.L. 2022. Steroidal alkaloid biosynthesis is coordinately regulated and differs among tomatoes in the red-fruited clade. The Plant Genome. https://doi.org/10.1002/tpg2.20192.

Pressure-mediated reflection spectroscopy criterion validity as a biomarker of fruit and vegetable intake: A two-side cross-sectional study of four racial or ethnic groups - (Peer Reviewed Journal)
Jilcott Pitts, S.B., Moran, N.E., Wu, Q., Harnack, L., Craft, N.E., Hanchard, N., Bell, R., Moe, S.G., Johnson, N., Obasohan, J., Carr-Manthe, P.L., Laska, M.N. 2021. Pressure-mediated reflection spectroscopy criterion validity as a biomarker of fruit and vegetable intake: A two-side cross-sectional study of four racial or ethnic groups. Journal of Nutrition. https://doi.org/10.1093/jn/nxab349.

Milking miRNAs for all their worth - (Other)
Hirschi, K.D. 2022. Milking miRNAs for all their worth. Journal of Nutrition. https://doi.org/10.1093/jn/nxab326.

Recommendations for the use of the veggie meter for spectroscopy-based skin carotenoid measurements in the research setting - (Peer Reviewed Journal)
Radtke, M.D., Poe, M., Stookey, J., Jilcott Pitts, S., Moran, N.E., Landry, M.J., Rubin, L.P., Scherr, R.E. 2021. Recommendations for the use of the veggie meter for spectroscopy-based skin carotenoid measurements in the research setting. Current Developments in Nutrition. 5(8). Article nzab104. https://doi.org/10.1093/cdn/nzab104.

Crucial role of mammalian glutaredoxin 3 in cardiac energy metabolism in diet-induced obese mice revealed by transcriptome analysis - (Peer Reviewed Journal)
Cheng, N., Mo, Q., Donelson, J., Wang, L., Breton, G., Rodney, G.G., Wang, J., Hirschi, K.D., Wehrens, X., Nakata, P.A. 2021. Crucial role of mammalian glutaredoxin 3 in cardiac energy metabolism in diet-induced obese mice revealed by transcriptome analysis. International Journal of Biological Sciences. 17(11):2871-2883. https://doi.org/10.7150/ijbs.60263.

An arabidopsis oxalyl-CoA decarboxylase, AtOXC, is important for oxalate catabolism in plants - (Peer Reviewed Journal)
Foster, J., Cheng, N., Paris, V., Wang, L., Wang, J., Wang, X., Nakata, P.A. 2021. An arabidopsis oxalyl-CoA decarboxylase, AtOXC, is important for oxalate catabolism in plants. International Journal of Molecular Sciences. 22(6):3266. https://doi.org/10.3390/ijms22063266.

Fusobacterium nucleatum secretes outer membrane vesicles and promotes intestinal inflammation - (Peer Reviewed Journal)
Engevik, M., Danhof, H., Ruan, W., Engevik, A.C., Chang-Graham, A.L., Engevik, K., Shi, Z., Zhao, Y., Brand, C.K., Krystofiak, E.S., Venable, S., Liu, X., Hirschi, K.D., Hyser, J.M., Spinler, J.K., Britton, R.A., Versalovic, J. 2021. Fusobacterium nucleatum secretes outer membrane vesicles and promotes intestinal inflammation. American Society for Microbiology. 12(2):e02706-e02720. https://doi.org/10.1128/mBio.02706-20.

Roles of regulatory RNAs in nutritional control - (Review Article)
McNeill, E.M., Hirschi, K.D. 2020. Roles of regulatory RNAs in nutritional control. Annual Review of Nutrition. 40:77-104. https://doi.org/10.1146/annurev-nutr-122319-035633.

In vitro digestion and cellular antioxidant activity of beta-carotene-loaded emulsion stabilized by soy protein isolate-Pleurotus eryngii polysaccharide conjugates - (Peer Reviewed Journal)
Hu, Q., Wu, Y., Zhong, L., Ma, N., Zhao, L., Ma, G., Cheng, N., Nakata, P.A., Xu, J. 2020. In vitro digestion and cellular antioxidant activity of beta-carotene-loaded emulsion stabilized by soy protein isolate-Pleurotus eryngii polysaccharide conjugates. Food Hydrocolloids. 112:106340. https://doi.org/10.1016/j.foodhyd.2020.106340.

Genetically modified plants: Nutritious, sustainable, yet underrated - (Review Article)
Hirschi, K.D. 2020. Genetically modified plants: Nutritious, sustainable, yet underrated. Journal of Nutrition. https://doi.org/10.1093/jn/nxaa220.

Gut bacteria have a novel sweet tooth: Ribose sensing and scavanging from fiber - (Other)
Karri, V., Hirschi, K.D. 2020. Gut bacteria have a novel sweet tooth: Ribose sensing and scavanging from fiber. Gut Microbes. https://doi.org/10.1080/19490976.2020.1770667.

Alteration of iron responsive gene expression in Arabidopsis glutaredoxin S17 loss of function plants with or without iron stress - (Peer Reviewed Journal)
Cheng, N., Yu, H., Rao, X., Park, S., Connolly, E.L., Hirschi, K.D., Nakata, P.A. 2020. Alteration of iron responsive gene expression in Arabidopsis glutaredoxin S17 loss of function plants with or without iron stress. Plant Signaling and Behavior. https://doi.org/10.1080/15592324.2020.1758455.

Dietary modulation of the gut microbiome - Probing the role of small RNAs - (Review Article)
Yang, J., Hirschi, K.D., Vivekanudeep, K., Spinler, J.K. 2020. Dietary modulation of the gut microbiome - Probing the role of small RNAs. Reference Module in Life Sciences. https://doi.org/10.1016/B978-0-08-100596-5.22722-4.

Expression of small interfering RNAs in lettuce using artificial microRNA technology - (Peer Reviewed Journal)
Kakeshpour, T., Tamang, T., Park, W.D., Manohar, M., Yang, J., Hirschi, K.D., Park, S. 2020. Expression of small interfering RNAs in lettuce using artificial microRNA technology. Biotechniques. 68(4). https://doi.org/10.2144/btn-2019-0139.