Research Project: Technologies for the Detection of Bacterial and Plant Toxins and Allergens that Impact Food Safety and Food Defense

Location: Foodborne Toxin Detection and Prevention Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Rapid analysis of amatoxins in human urine by means of affinity column chromatography and liquid chromatography-high-resolution tandem mass spectrometry - (Peer Reviewed Journal)
Vollmer, A.C., Fecher-Trost, C., Bever, C.S., Tam, C.C., Wagmann, L., Meyer, M.R. 2024. Analysis of amatoxins in human urine after magnetic bead-based immunoprecipitation and liquid chromatography high-resolution tandem mass-spectrometry. Scientific Reports. 14(1). Article 21397. https://doi.org/10.1038/s41598-024-72463-3.

Rapid and sensitive detection of Shiga-toxin Escherichia coli (STEC) from food matrices using the CANARY biosensor assay - (Peer Reviewed Journal)
Tam, C.C., Du, W.N., Wang, Y., Flannery, A.R., He, X. 2024. Rapid and sensitive detection of Shiga-toxin Escherichia coli (STEC) from food matrices using the CANARY biosensor assay. Toxins. 16,325. https://doi.org/10.3390/toxins16070325.

Streptococcal pyrogenic exotoxin B is a superantigen that induces murinesplenocyte proliferation and secretion of IL-2 and IFN-¿¿ ex vivo - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2024. Streptococcal pyrogenic exotoxin B is a superantigen that induces murinesplenocyte proliferation and secretion of IL-2 and IFN-¿¿ ex vivo. FEMS Microbiology Letters. 371.Article fnae036. https://doi.org/10.1093/femsle/fnae036.

Chemical composition of turmeric (Curcuma longa L.) ethanol extract and its antimicrobial activities and free radical scavenging capacities - (Peer Reviewed Journal)
Wu, H., Liu, Z., Zhang, Y., Gao, B., Li, Y., He, X., Sun, J., Choe, U., Chen, P., Blaustein, R., Yu, L. 2024. Chemical composition of turmeric (Curcuma longa L.) ethanol extract and its antimicrobial activities and free radical scavenging capacities. Foods. 13(10). Article 1550. https://doi.org/10.3390/foods13101550.

Development of a rapid and sensitive CANARY biosensor assay for the detection of Shiga toxin 2 from Escherichia coli - (Peer Reviewed Journal)
Tam, C.C., Wang, Y., Du, W.N., Flannery, A.R., He, X. 2024. Development of a rapid and sensitive CANARY biosensor assay for the detection of Shiga toxin 2 from Escherichia coli. Toxins. 16(3). Article 148. https://doi.org/10.3390/toxins16030148.

Chemical compositions of Scutellaria baicalensis Georgi. (Huangqin) extracts and their effects on ACE2 binding of SARS-CoV-2 spike protein, ACE2 activity, and free radicals - (Peer Reviewed Journal)
Gao, B., Zhu, H., Liu, Z., He, X., Sun, J., Li, Y., Wu, X., Pehrsson, P.R., Zhang, Y., Yu, L. 2024. Chemical compositions of Scutellaria baicalensis Georgi. (Huangqin) extracts and their effects on ACE2 binding of SARS-CoV-2 spike protein, ACE2 activity, and free radicals. International Journal of Molecular Sciences. 25(4). Article 2045. https://doi.org/10.3390/ijms25042045.

Detection of cleaved Stx2a in the blood of STEC-infected patients - (Peer Reviewed Journal)
Varrone, E., Carnicelli, D., He, X., Grasse, M., Stampfer, K., Huber, S., Kellnerova, S., Tazzari, P., Ricci, F., Paterini, P., Ardissino, G., Morabito, S., Orth-Holler, D., Wurzner, R., Brigotti, M. 2023. Detection of cleaved Stx2a in the blood of STEC-infected patients. Toxins. 15(12). Article 690. https://doi.org/10.3390/toxins15120690.

A novel Shiga toxin 2a neutralizing antibody therapeutic with low immunogenicity and high efficacyA novel Shiga toxin 2a neutralizing antibody therapeutic with low immunogenicity and high efficacy - (Peer Reviewed Journal)
Kirkland, M., Patfield, S., Hughes, A.C., Hernlem, B.J., He, X. 2023. A novel Shiga toxin 2a neutralizing antibody therapeutic with low immunogenicity and high efficacyA novel Shiga toxin 2a neutralizing antibody therapeutic with low immunogenicity and high efficacy. Antimicrobial Agents and Chemotherapy. 68(1). https://doi.org/10.1128/aac.00598-23.

Enzymatic cleavage of Stx2a in the gut and identification of pancreatic elastase and trypsin as possible main cleavers - (Peer Reviewed Journal)
Kellnerova, S., Huber, S., Massri, M., Fleischer, V., Losso, K., Sarg, B., Kremser, L., Talasz, H., He, X., Varrone, E., Brigotti, M., Ardissino, G., Orth-Holler, D., Wurzner, R. 2023. Enzymatic cleavage of Stx2a in the gut and identification of pancreatic elastase and trypsin as possible main cleavers. Microorganisms. 11(10). Article 2487. https://doi.org/10.3390/microorganisms11102487.

Development of thermally stable nanobodies for detection and neutralization of Staphylococcal enterotoxin B - (Peer Reviewed Journal)
Hughes, A.C., Kirkland, M., Du, W.N., Rasooly, R., Hernlem, B.J., Tam, C.C., Zhang, Y., He, X. 2023. Development of thermally stable nanobodies for detection and neutralization of Staphylococcal enterotoxin B. Toxins. 15(6). Article 400. https://doi.org/10.3390/toxins15060400.

TMT-based quantitative proteomic and scanning electron microscopy reveals biological and morphological changes of Staphylococcus aureus irradiated by electron beam - (Peer Reviewed Journal)
Zheng, Q., Chen, Z., Yan, W., Wang, H., Tian, W., Feng, D., Yue, L., Qi, W., He, X., Kong, Q. 2023. TMT-based quantitative proteomic and scanning electron microscopy reveals biological and morphological changes of Staphylococcus aureus irradiated by electron beam. LWT - Food Science and Technology. 184. Article 114977. https://doi.org/10.1016/j.lwt.2023.114977.

Chemical composition of honeysuckle (Lonicerae japonicae) extracts and their potential in inhibiting the SARS-CoV-2 spike protein and ACE2 binding, suppressing ACE2, and scavenging radicals - (Peer Reviewed Journal)
Gao, B., Zhu, L., Liu, Z., Li, Y., He, X., Wu, X., Pehrsson, P.R., Sun, J., Xie, Z., Slavin, M., Yu, L. 2023. Chemical composition of honeysuckle (Lonicerae japonicae) extracts and their potential in inhibiting the SARS-CoV-2 spike protein and ACE2 binding, suppressing ACE2, and scavenging radicals. Journal of Agricultural and Food Chemistry. 71(15):6133-6143. https://doi.org/10.1021/acs.jafc.3c00584.

A three-monoclonal antibody combination potently neutralizes BoNT/G toxin in mice - (Peer Reviewed Journal)
Fan, Y., Lou, J., Tam, C.C., Wen, W., Conrad, F., Alves, P., Cheng, L.W., Garcia-Rodriguez, C., Farr-Jones, S., Marks, J.D. 2023. A three-monoclonal antibody combination potently neutralizes BoNT/G toxin in mice. Toxins. 15(5). Article 316. https://doi.org/10.3390/toxins15050316.

Chemical compositions of clove (Syzygium aromaticum (L.) Merr. & L.) extracts and their potentials in suppressing SARS-CoV-2 spike protein-ACE2 binding, inhibiting ACE2, and scavenging free radicals - (Peer Reviewed Journal)
Li, Y., Liu, Z., Zeng, M., El Kadiri, A., Huang, J., Kim, A., He, X., Sun, J., Chen, P., Wang, T.T., Zhang, Y., Gao, B., Xie, Z., Yu, L. 2022. Chemical compositions of clove (Syzygium aromaticum (L.) Merr. & L.) extracts and their potentials in suppressing SARS-CoV-2 spike protein-ACE2 binding, inhibiting ACE2, and scavenging free radicals. Journal of Agricultural and Food Chemistry. 70(45):14403-14413. https://doi.org/10.1021/acs.jafc.2c06300.

Effect of thermal treatments on soluble-free, soluble-conjugated, and insoluble-bound phenolic components and free radical scavenging properties of tomato seeds - (Peer Reviewed Journal)
Li, Y., Wu, K., Liu, Z., He, X., Sun, J., Zeng, M., Lee, S., Wang, T.T., Gao, B., Xie, Z., Wei, C., Yu, L. 2022. Effect of thermal treatments on soluble-free, soluble-conjugated, and insoluble-bound phenolic components and free radical scavenging properties of tomato seeds. ACS Food Science and Technology. 2(10):1631-1638. https://doi.org/10.1021/acsfoodscitech.2c00228.

Identification of soluble free, soluble conjugated and insoluble bound phenolics in tomato seeds and their radical scavenging and antiproliferative activities - (Peer Reviewed Journal)
Li, Y., Liu, Z., Tamia, G., He, X., Sun, J., Chen, P., Lee, S., Wang, T.T., Gao, B., Xie, Z., Yu, L. 2022. Identification of soluble free, soluble conjugated and insoluble bound phenolics in tomato seeds and their radical scavenging and antiproliferative activities. Journal of Agricultural and Food Chemistry. 70(29):9039-9047. https://pubs.acs.org/doi/10.1021/acs.jafc.2c03418.

Evaluation of a witch hazel extract for the potential prebiotic and protective effect on select Lactiplantibacillus plantarum (prev. Lactobacillus plantarum) strains - (Peer Reviewed Journal)
Failla, M., Lee, J., Rasooly, R., Apostolidis, E. 2022. Evaluation of a witch hazel extract for the potential prebiotic and protective effect on select Lactiplantibacillus plantarum (prev. Lactobacillus plantarum) strains. Frontiers in Nutrition. 9. Article 874666. https://doi.org/10.3389/fnut.2022.874666.

Human leukemia T-cell lines as alternative to animal use for detecting biologically active Staphylococcal enterotoxin type B - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2021. Human leukemia T-cell lines as alternative to animal use for detecting biologically active Staphylococcal enterotoxin type B. Toxins. 13(5). Article 300. https://doi.org/10.3390/toxins13050300.

Prevalence and genetic analysis of chromosomal mcr-3/7 in Aeromonas from U.S. animal-derived samples - (Peer Reviewed Journal)
Wang, Y., Hou, N., Rasooly, R., Gu, Y.Q., He, X. 2021. Prevalence and genetic analysis of chromosomal mcr-3/7 in Aeromonas from U.S. animal-derived samples. Frontiers in Microbiology. 12. Article 667406. https://doi.org/10.3389/fmicb.2021.667406.