Location: Horticultural Crops Production and Genetic Improvement Research Unit
Project Number: 2072-21000-055-017-G
Project Type: Grant
Start Date: Aug 1, 2020
End Date: Oct 31, 2022
Objective:
Development of a large population of infertile hexaploids (Hibiscus) from which to select cultivars for the nursery industry.
Approach:
In a previous study, seventy cross combinations between available cultivars and octoploids, including Aphrodite and Bali, were developed and resulted in 1,140 seedlings. The entire population of these seedlings needs to be evaluated to determine ploidy level in order to evaluate plants of ploidy levels likely to be reduced in fertility, specifically 5x and 6x, with an emphasis on the latter. Ploidy level of the seedlings will be determined using flow cytometry analysis of DAPI-stained nuclei. Analysis will be conducted using the QuantumP flow cytometer.
