Research Project: Management and Biology of Arthropod Pests and Arthropod-borne Plant Pathogens

Location: Emerging Pests and Pathogens Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Phenology, voltinism and brood development of Xylosandrus (Coleoptera: Curculionidae) ambrosia beetles in New York - (Peer Reviewed Journal)
Milbrath, L.R., Biazzo, J. 2024. Phenology, voltinism and brood development of Xylosandrus (Coleoptera: Curculionidae) ambrosia beetles in New York. Journal of Insect Science. Vol. 24, Issue 5. https://doi.org/10.1093/jisesa/ieae102.

Emerald ash borer management and research: decades of damage and still expanding - (Literature Review)
Jianghua, S., Tuuli-Marjaana, K., Wickham, J.D., Baranchikov, Y.N., Bushley, K.E. 2024. Emerald ash borer management and research: decades of damage and still expanding. Annual Review of Entomology. 69:239-258. https://doi.org/10.1146/annurev-ento-012323-032231.

Phenology and voltinism of the ambrosia beetle Anisandrus maiche in central New York - (Abstract Only)
Milbrath, L.R., Biazzo, J. 2024. Phenology and voltinism of the ambrosia beetle Anisandrus maiche in central New York. Entomological Society of America Annual Meeting. https://entomology2024.eventscribe.net/fsPopup.asp?PresentationID=1470794&mode=presInfo.

Data mining redefines the timeline and geographic spread of cotton leafroll dwarf virus - (Peer Reviewed Journal)

Wolbachia confers protection against the entomopathogenic fungus Metarhizium pingshaense in African Aedes aegypti - (Peer Reviewed Journal)
Bilgo, E., Mancini, M.V., Gnambani, E.J., Dokpomiwa, H.A., Murdochy, S., Lovett, B.R., St. Leger, R.J., Sinkins, S.P., Diabate, A. 2024. Wolbachia confers protection against the entomopathogenic fungus Metarhizium pingshaense in African Aedes aegypti. Environmental Microbiology Reports. 16(4), e13316. https://doi.org/10.1111/1758-2229.13316.

History of Beauveria bassiana and Metarhizium anisopliae: Resolving historical problems and anticipating future challenges - (Abstract Only)
Bushley, K.E., Rehner, S.A. 2024. History of Beauveria bassiana and Metarhizium anisopliae: Resolving historical problems and anticipating future challenges. Society of Invertebrate Pathology. 56th Annual Society of Invertebrate Pathology Meeting, July 28 - August 1, 2024.

Fungal community and metabolomic investigations reveal ash metabolites shaping phloem fungal communities in ash trees (Fraxinus spp.) resistant and susceptible against highly invasive emerald ash borer (Agrilus planipennis) - (Peer Reviewed Journal)
Koshi, T., Zhang, B., Mogouong, J., Wang, H., Chen, Z., Li, H., Bushley, K.E., Sun, J. 2024. Fungal community and metabolomic investigations reveal ash metabolites shaping phloem fungal communities in ash trees (Fraxinus spp.) resistant and susceptible against highly invasive emerald ash borer (Agrilus planipennis). Plant Cell and Environment. 1-19. https://doi.org/10.1111/pce.14996.

Fungal parasites of nematode eggs for integrated pest management of soybean cyst nematode - (Abstract Only)
Green, E.A., Kim, D., Haarith, D., Knight, N., Chen, S., Bushley, K.E. 2024. Fungal parasites of nematode eggs for integrated pest management of soybean cyst nematode. Society for Invertebrate Pathology Annual Meeting. 56th Annuanl Society of Invertebrate Pathology Meeting, July 28 - August 1, 2024.

Repertoires of Secondary Metabolite Biosynthetic Gene Clusters in Insect and Nematode Parasitic Fungi - (Abstract Only)
Bushley, K.E., Showalter, D., Olarte, R., Rehner, S.A. 2024. Repertoires of Secondary Metabolite Biosynthetic Gene Clusters in Insect and Nematode Parasitic Fungi. Mycological Society of America. Mycological Society of America Annual Meeting, June 9-12, 2024.

Investigating the soybean cyst nematode microbiome for biocontrol organisms - (Abstract Only)

Genome-wide association study reveals genes regulating vector competency of Diaphorina citri, insect vector of Huanglongbing (HLB) - (Abstract Only)

Trunk injection of commercial oxytetracycline for HLB management in young Grapefruit trees using a novel injection system - (Abstract Only)

Symbiont Technology: development and optimization of a novel delivery approach for therapeutics to control Candidatus Liberibacter asiaticus - (Abstract Only)

Flipping the HLB therapeutic screening paradigm: That’s right we went straight to the field - (Abstract Only)

Understanding and Engineering Symbiont Physiology with Arabidopsis Cell Identity Lines and Post Transcriptional Gene Silencing - (Abstract Only)

Nanometal reagent therapeutics suppress Candidatus Liberibacter asiaticus, the causal pathogen of Huanglongbing - (Abstract Only)

Genomic Architecture of Fungal Metabolism Involved in Host and Ecological Specialization - (Abstract Only)
Rodrigo, O., Malvick, D.K., Bushley, K.E. 2024. Genomic Architecture of Fungal Metabolism Involved in Host and Ecological Specialization. Fungal Genetics Conference. 32nd Fungal Genetics Conference, March 12-17,2024.

In-gallery social behaviors of the ambrosia beetle Xylosandrus germanus (Coleoptera: Curculionidae) - (Peer Reviewed Journal)
Milbrath, L.R., Biazzo, J., Mudrak, E. 2024. In-gallery social behaviors of the ambrosia beetle Xylosandrus germanus (Coleoptera: Curculionidae). Environmental Entomology. 53(1):85-93. https://doi.org/10.1093/ee/nvae003.

Potential for Use of Species in the Subfamily Erynioideae for Biological Control and Biotechnology - (Peer Reviewed Journal)
Gryganskyi, A.P., Hajek, A.E., Voloschuk, N., Idnurm, A., Eilenberg, J., Manrino, R.G., Bushley, K.E., Kava, L., Kutovenko, V.B., Nie, Y., Anike, F. 2024. Potential for Use of Species in the Subfamily Erynioideae for Biological Control and Biotechnology. Microorganisms. 12(1):168. https://doi.org/10.3390/microorganisms12010168.

Response of black swallowwort (Vincetoxicum nigrum) to herbicides plus mowing - (Peer Reviewed Journal)
Milbrath, L.R., Biazzo, J., Morris, S.H., Ditommaso, A. 2023. Response of black swallowwort (Vincetoxicum nigrum) to herbicides plus mowing. Invasive Plant Science and Management. 15:161-167. https://doi.org/10.1017/inp.2022.27.

Killing them softly: putting the fungal squeeze on cyst nematodes - (Abstract Only)

A comprehensive framework for the delimitation of species within the Bemisia tabaci cryptic complex, a global pest-species group - (Peer Reviewed Journal)

Swallow-wort biocontrol agents: history and ecology in North America - (Other)
Tewksbury, L., Szucs, M., Milbrath, L.R., Bourchier, R.S., Cappuccino, N. 2023. Swallow-wort biocontrol agents: history and ecology in North America. North American Invasive Species Management Association (NAISMA). NAISMA-BCW-2023-30-SWALLOW-WORTS-P.

Swallow-worts (Vincetoxicum spp.): history and ecology in North America - (Other)
Tewsbury, L., Szucs, M., Milbrath, L.R., Bourchier, R.S., Cappuccino, N. 2023. Swallow-worts (Vincetoxicum spp.): history and ecology in North America. North American Invasive Species Management Association (NAISMA). https://bugwoodcloud.org/resource/files/27697.pdf.

Characterization of cotton virus A, a distinct member of the caulimovirus genus with endogenous viral elements in upland cotton - (Pre-print Publication)
West-Ortiz, M., Stuehler, D., Pollock, E., Wilson, J.R., Preising, S., Alabi, O., Fuchs, M., Heck, M.L., Olmedo-Velarde, A. 2023. Characterization of cotton virus A, a distinct member of the caulimovirus genus with endogenous viral elements in upland cotton. bioRxiv. https://doi.org/10.1101/2023.06.14.544975.

Microbiome of North American ash for biocontrol of emerald ash borer - (Abstract Only)

Development of genetic and genomic resources for the ARS entomopathogenic fungi collection - (Abstract Only)

Profiling plant proteome and transcriptome changes during grapevine fanlead virus infection - (Peer Reviewed Journal)
Roy, B., Deblasio, S.L., Yang, Y., Thannhauser, T.W., Heck, M.L., Fuchs, M. 2023. Profiling plant proteome and transcriptome changes during grapevine fanlead virus infection. Journal of Proteome Research. 22(6):1997-2017. https://doi.org/10.1021/acs.jproteome.3c00069?urlappend=%3Fref%3DPDF&jav=VoR&rel=cite-as.

Potato leafroll virus molecular interactions with plants and aphids: gaining a new tactical advantage on an old foe - (Review Article)
Olmedo-Velarde, A., Wilson, J.R., Stallone, M., Deblasio, S.L., Chappie, J.S., Heck, M.L. 2023. Potato leafroll virus molecular interactions with plants and aphids: gaining a new tactical advantage on an old foe. Physiological and Molecular Plant Pathology. https://doi.org/10.1016/j.pmpp.2023.102015.

Grapevine fanleaf virus RNA1-encoded proteins 1A and 1BHel individually or cooperatively suppress RNA silencing - (Peer Reviewed Journal)
Choi, J., Pakbaz, S., Yepes, L., Cieniewicz, E., Schmitt-Keichinger, C., Labarile, R., Minutillo, S., Heck, M.L., Hua, J., Fuchs, M. 2023. Grapevine fanleaf virus RNA1-encoded proteins 1A and 1BHel individually or cooperatively suppress RNA silencing. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/MPMI-01-23-0008-R.

Invasion is not a monolith: invasion effects by Vincetoxicum rossicum (Kleopow) Barbar. differ based on background environment - (Abstract Only)
Schnurr, J.L., Biazzo, J., Milbrath, L.R. 2023. Invasion is not a monolith: invasion effects by Vincetoxicum rossicum (Kleopow) Barbar. differ based on background environment. Ecological Society of America Abstracts. PS 11-146.

Closely related invasive species may be controlled by the same demographic life stages - (Peer Reviewed Journal)
Molofsky, J., Thom, D., Keller, S.R., Milbrath, L.R. 2023. Closely related invasive species may be controlled by the same demographic life stages. NeoBiota. 82:189-207. https://doi.org/10.3897/neobiota.82.95127.

A perspective on current therapeutic molecule screening methods against ‘candidatus liberibacter asiaticus’, the presumed causative agent of citrus Huanglongbing - (Peer Reviewed Journal)
Kennedy, J.P., Wood, K., Pitino, M., Mandadi, K., Igwe, D., Shatters, R.G., Widmer, T.L., Niedz, R.P., Heck, M.L. 2023. A perspective on current therapeutic molecule screening methods against ‘candidatus liberibacter asiaticus’, the presumed causative agent of citrus Huanglongbing. Phytopathology. https://doi.org/10.1094/PHYTO-12-22-0455-PER.

Potato leafroll virus modulates aphid infection with a newly described insect flavivirus - (Peer Reviewed Journal)
Larrea-Sarmiento, A., Olmedo-Velarde, A., Preising, S., West-Ortiz, M., Fei, Z., Heck, M.L. 2023. Potato leafroll virus modulates aphid infection with a newly described insect flavivirus. Phytopathology. 125:102015. https://doi.org/10.1016/j.pmpp.2023.102015.

Biology of invasive plants 3: Vincetoxicum nigrum (L.) Moench and Vincetoxicum rossicum (Kleopow) Barbarich - (Peer Reviewed Journal)
Westbrook, A.S., Milbrath, L.R., Weinberg, J., Ditommaso, A. 2023. Biology of invasive plants 3: Vincetoxicum nigrum (L.) Moench and Vincetoxicum rossicum (Kleopow) Barbarich. Journal of Invasive Plant Science Management. 16:3-26. https://doi.org/10.1017/inp.2023.7.

Sources of fungal symbionts in the microbiome of a mobile insect host, spodoptera frugiperda - (Peer Reviewed Journal)
Watson, M., May, G., Bushley, K.E. 2022. Sources of fungal symbionts in the microbiome of a mobile insect host, spodoptera frugiperda. Microbial Ecology. 86:900-913. https://doi.org/10.1007/s00248-022-02140-3.

Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids - (Peer Reviewed Journal)
Schiltz, C.J., Wilson, J.R., Hosford, C., Adams, M.C., Preising, S.E., Deblasio, S.L., Maccleod, H.J., Ven Eck, J., Heck, M.L., Chappie, J.S. 2022. Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids. Nature Communications. 13:6368. https://doi.org/10.1038/s41467-022-33979-2.

Ecology drives the observed spectrum of hydrophobin protein diversity across kingdom fungi - (Pre-print Publication)
Lovett, B.R., Kasson, M.T., Gandier, J. 2022. Ecology drives the observed spectrum of hydrophobin protein diversity across kingdom fungi. bioRxiv. https://doi.org/10.1101/2022.08.19.504535.

Phenology, voltinism and brood development of the ambrosia beetle Xylosandrus germanus in New York - (Abstract Only)
Milbrath, L.R., Biazzo, J. 2022. Phenology, voltinism and brood development of the ambrosia beetle Xylosandrus germanus in New York. Meeting Abstract. D3431. https://www.eventscribe.net/2022/jam2022/fsPopup.asp?Mode=posterinfo&PosterID=499482.

Strain tracking of ‘candidatus liberibacter asiaticus’, citrus greening disease pathogen, enabled by high-resolution microbiome analysis of the Asian citrus psyllid - (Peer Reviewed Journal)
Higgins, S., Mann, M., Heck, M.L. 2022. Strain tracking of ‘candidatus liberibacter asiaticus’, citrus greening disease pathogen, enabled by high-resolution microbiome analysis of the Asian citrus psyllid. Phytopathology. 112(11):2273-2287. https://doi.org/10.1094/PHYTO-02-22-0067-R.

Transmission of grapevine red blotch virus by spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between free-living vines and vitis vinifera ‘cabernet franc’ - (Peer Reviewed Journal)
Hoyle, V., Flasco, M., Choi, J., Cieniewicz, E., Mclane, H., Perry, K., Dangl, G., Rwahnih, M., Heck, M.L., Loeb, G., Fuchs, M. 2022. Transmission of grapevine red blotch virus by spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between free-living vines and vitis vinifera ‘cabernet franc’. Viruses. 14: Article e1156. https://doi.org/10.3390/v14061156.

The impacts of a perennial, summer growing invasive species, pale swallow-wort (Vincetoxicum rossicum (Kleopow) Barbar.), on spring ephemeral populations - (Abstract Only)
Schnurr, J., Biazzo, J., Milbrath, L.R. 2022. The impacts of a perennial, summer growing invasive species, pale swallow-wort (Vincetoxicum rossicum (Kleopow) Barbar.), on spring ephemeral populations. Ecological Society of America Abstracts. PS 39-11. https://www.eventscribe.net/2022/ESA/fsPopup.asp?Mode=posterinfo&PosterID=501852.

Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids - (Pre-print Publication)
Schiltz, C.J., Wilson, J.R., Hosford, C.J., Adams, M., Preising, S., Deblasio, S.L., Maccleod, H.J., Van Eck, J., Heck, M.L., Chappie, J.S. 2022. Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids. bioRxiv. https://doi.org/10.1101/2022.04.10.487772.

Lessons learned about the biology and genomics of Diaphorina citri infection with “Candidatus Liberibacter asiaticus” by integrating new and archived organ-specific transcriptome data - (Peer Reviewed Journal)
Mann, M., Saha, S., Pitino, M., Moulton, K.M., Cano, L., Hunter, W.B., Mueller, L.A., Heck, M.L. 2022. Lessons learned about the biology and genomics of Diaphorina citri infection with “Candidatus Liberibacter asiaticus” by integrating new and archived organ-specific transcriptome data. Gigascience. 11:1-16. https://doi.org/10.1093/gigascience/giac035.

Quantitative isotope-labeled cross-linker proteomics reveals developmental variation in protein interactions and post-translational modifications in diaphorina citri, the citrus greening insect vector - (Peer Reviewed Journal)
Ramsey, J.S., Zhong, X., Saha, S., Chavez, J., Johnson, R., Mahoney, J., Keller, A., Moulton, K., Mueller, L., Hall, D.G., Maccoss, M., Bruce, J., Heck, M.L. 2022. Quantitative isotope-labeled cross-linker proteomics reveals developmental variation in protein interactions and post-translational modifications in diaphorina citri, the citrus greening insect vector. ACS Agricultural Science and Technology. 2(3):486-500. https://doi.org/10.1021/acsagscitech.1c00264.

Genetic diversity and aggressiveness of Fusarium virguliforme isolates across the Midwestern United States - (Peer Reviewed Journal)
Olarte, R.A., Hall, R., Tabima, J., Malvick, D., Bushley, K.E. 2021. Genetic diversity and aggressiveness of Fusarium virguliforme isolates across the Midwestern United States. Phytopathology. https://doi.org/10.1094/PHYTO-05-21-0191-R.

Crystal structure of the potato leafroll virus coat protein and implications for viral assembly - (Peer Reviewed Journal)
Adams, M., Schiltz, C., Heck, M.L., Chappie, J. 2021. Crystal structure of the potato leafroll virus coat protein and implications for viral assembly. Journal of Structural Biology. 214(1):107811. https://doi.org/10.1016/j.jsb.2021.107811.

Omnivory in predatory lady beetles is widespread and driven by an appetite for sterols - (Peer Reviewed Journal)
Ugine, T., Krasnoff, S., Losey, J., Behmer, S.T. 2022. Omnivory in predatory lady beetles is widespread and driven by an appetite for sterols. Functional Ecology. 36(2):458-470. https://doi.org/10.1111/1365-2435.13965.

Southern blight of perennial swallowwort (Vincetoxicum spp.) in New York - (Peer Reviewed Journal)
Pethybridge, S.J., Sharma, S., Murphy, S., Biazzo, J., Milbrath, L.R. 2021. Southern blight of perennial swallowwort (Vincetoxicum spp.) in New York. Invasive Plant Science and Management. 14(4):223–231. https://doi.org/10.1017/inp.2021.30.

Host plant adaptation drives changes in Diaphorina citri proteome regulation, proteoform expression and transmission of Candidatus Liberibacter asiaticus, the citrus greening pathogen - (Peer Reviewed Journal)
Ramsey, J.S., Ammar, D., Mahoney, J.E., Rivera, K., Johnson, R., Igwe, D.O., Thannhauser, T.W., Maccoss, M.J., Hall, D.G., Heck, M.L. 2022. Host plant adaptation drives changes in Diaphorina citri proteome regulation, proteoform expression and transmission of Candidatus Liberibacter asiaticus, the citrus greening pathogen. Phytopathology. 112:101-115. https://doi.org/10.1094/phyto-06-21-0275-r.

An excised leaf assay to measure acquisition of “candidatus liberibacter asiaticus” by psyllids associated with citrus Huanglongbing disease - (Peer Reviewed Journal)
Igwe, D.O., Higgins, S.A., Heck, M.L. 2021. An excised leaf assay to measure acquisition of “candidatus liberibacter asiaticus” by psyllids associated with citrus Huanglongbing disease. Phytopathology. in press. https://doi.org/10.1094/PHYTO-03-21-0124-SC.

Growth chamber and greenhouse screening of promising in vitro fungal biological control candidates for the soybean cyst nematode (Heterodera glycines) - (Peer Reviewed Journal)
Haarith, D., Kim, D., Chen, S., Bushley, K.E. 2021. Growth chamber and greenhouse screening of promising in vitro fungal biological control candidates for the soybean cyst nematode (Heterodera glycines). Biological Control. 160:104635. https://doi.org/10.1016/j.biocontrol.2021.104635.

Proteomic polyphenism in color morphotypes of diaphorina citri, insect vector of citrus greening disease - (Peer Reviewed Journal)
Hosseinzadeh, S., Higgins, S.A., Ramsey, J.S., Howe, K.J., Griggs, M., Castrillo, L.A., Heck, M.L. 2021. Proteomic polyphenism in color morphotypes of diaphorina citri, insect vector of citrus greening disease. Journal of Proteome Research. 20(5):2851-2866. https://doi.org/10.1021/acs.jproteome.1c00089.

Grapevine red blotch virus is transmitted by the three-cornered alfalfa hopper in a circulative, nonpropagative mode with unique attributes - (Peer Reviewed Journal)
Flasco, M., Hoyle, V., Cieniewicz, E., Roy, B., Mclane, H., Perry, K., Loeb, G., Nault, B., Heck, M.L., Fuchs, M. 2021. Grapevine red blotch virus is transmitted by the three-cornered alfalfa hopper in a circulative, nonpropagative mode with unique attributes. Phytopathology. https://doi.org/10.1094/PHYTO-02-21-0061-R.