Location: Molecular Plant Pathology Laboratory
Project Number: 8042-21500-003-002-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 23, 2024
End Date: Sep 22, 2026
Objective:
Objective: Elucidate key genes and processes involved in alfalfa resistance to stem nematode Ditylenchus dipasaci.
Sub-objective 1: Decipher molecular mechanisms underlying alfalfa defense responses to Ditylenchus dipasaci.
Sub-objective 2: Identify and select genes of Ditylenchus dipasaci that could be targeted for nematode control in alfalfa.
Approach:
- Collection of Ditylenchus dipasaci field samples and culturing of the nematode (Cooperator).
- Next generation sequencing (NGS) of alfalfa accessions resistant and susceptible to stem nematode Ditylenchus dipasaci (Cooperator)
- Transcriptomic, proteomic and functional analysis of NGS data (ARS and Cooperator)