Location: Food and Feed Safety Research
Project Number: 3091-32000-037-069-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Dec 13, 2023
End Date: Jan 31, 2028
Objective:
1. Determine if continual feeding of an oregano-based feed additive reduces Salmonella invasion and/or colonization in young poultry.
2. Determine if continual feeding of an oregano-based feed additive reduces Salmonella colonization in slaughter-aged broilers.
3. Determine if continual feeding of an oregano-based supplement accompanied by water treatment during the finishing time reduces Salmonella colonization in slaughter-aged broilers.
Approach:
1. Determine if continual feeding of an oregano-based feed additive (0 and 300 g/ton) reduces Salmonella invasion and/or colonization in young poultry. All oregano supplementation will be in the form of powder mixed into the feed. At placement, all chicks will be provided the same starter diet with no feed additive. On Day 4, chicks will be placed on their assigned dietary treatment followed by a challenge with Salmonella enterica serovar Enteritidis on day 7. On days 11 and 21 chicks will be weighed, bled, and humanely euthanized and necropsied where the liver and ceca will be removed and enriched for Salmonella;cecal content will be analyzed for Salmonella enumeration, and various tissue samples collected, and flash frozen for future immunological studies.
2. Determine if continual feeding of an oregano-based feed additive reduces Salmonella colonization in slaughter-aged broilers. At placement, all chicks will be provided the same starter diet with no feed additive. The supplement will be in the form of powder mixed into the feed and provided at growth promoting dose (150 g/ton) from days 4-33 and at therapeutic dose (300 g/ton) from days 33-42. On Day 4, chicks will be placed on their assigned dietary treatment and challenged with Salmonella enterica serovar Enteritidis. The birds will be provided starter rations on days 4-14 birds (0 or 150 g/ton), followed by a grower diet (0 or 150 g/ton) from days 14-32, and lastly a finisher diet from days 32-42 with a higher level of supplementation (0 or 300 g/ton) to target and eliminate any remaining Salmonella. On day 42 birds will be weighed, bled, and humanely euthanized and necropsied where the liver and ceca will be removed and enriched for Salmonella; cecal content will be analyzed for Salmonella enumeration, and various tissue samples collected, and flash frozen for future immunological studies.
3. Determine if continual feeding of an oregano-based supplement accompanied by water treatment during the finishing time reduces Salmonella colonization in slaughter-aged broilers. At placement, all chicks will be provided the same starter diet with no feed additive. On Day 4, chicks will be placed on their assigned dietary treatment and challenged with Salmonella enterica serovar Enteritidis. The supplement will be in the form of powder mixed into the feed and provided at growth promoting dose (150 g/ton) from days 4-42 and in the water (6 oz/gal) days 33-42. The birds will be provided starter rations on days 4-14 birds (0 or 150 g/ton), followed by a grower diet (0 or 150 g/ton) from days 14-32, and lastly a finisher diet from days 32-42 (0 or 150 g/ton) with additional treatment provided in the drinking water (0 or 6 oz/gal) to target and eliminate any remaining Salmonella. On day 42 birds will be weighed, bled, and humanely euthanized and necropsied.
