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ARS Home » Research » Publications at this Location » Publication #109099
Title: MAPPING OF SEVEN BOVINE CYTOKINE GENES INVOLVED IN T-LYMPHOCYTE GROWTH,DIFFERENTIATION, AND IMMUNE RESPONSE.

Author
item Sonstegard, Tad
item Garrett, Wesley
item Bennett, Gary
item Kappes, Steven - Steve
item Zarlenga, Dante
item Gasbarre, Louis

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/3/2000
Publication Date: 12/1/2000
Citation: Sonstegard, T.S., Garrett, W.M., Bennett, G.L., Kappes, S.M., Zarlenga, D.S., Gasbarre, L.C. 2000. Mapping of seven bovine cytokine genes involved in t-lymphocyte growth, differentiation, and immune response. Animal Genetics. 31(6):406-408.

Interpretive Summary: Genes which encode for cytokines play important roles in regulating host immune responses to disease and other environmental stress factors and, as such, may play an equally important role in disease resistance and susceptibility traits in cattle. One method to enhance mapping these traits is to develop informative markers associated with candidate genes. Since relatively few cytokine loci have been positioned on the bovine genetic map, we determined physical and linkage map locations for seven cytokine genes using bacterial artificial chromosomes as resource for developing physical probes and genetic markers. This study improves the bovine comprehensive and comparative maps by nearly doubling the number of mapped loci that belong to genetic pathways involved with regulating host immunity. These microsatellite markers provide a resource for incorporating cytokine candidate genes into a genome wide analysis of resource cattle populations segregating for disease resistance and susceptibility traits.

Technical Abstract: Genes which encode for cytokines play important roles in regulating host immune responses to disease an other environmental stress factors and, as such, may play an equally important role in disease resistance and susceptibility traits in cattle. However, relatively few cytokine lock have been positioned on the bovine genetic map. To improve upon this, we determined physical and linkage map locations for seven cytokine genes that are specifically involved with growth and differentiation of T- lymphocytes or T-cell initiated immune responses that drive or direct the proper effector cell populations. Bovine cDNAs were used as probes to isolate unique clones from a bovine bacterial artificial chromosome (BAC) library. These BAC clones were used as a DNA source to generate probes for fluorescent in situ hybridization (FISH) and to develop microsatellite markers. Physical assignment by FISH were performed for all seven cytokine loci, and alignment of bovine human maps based on these assignments agreed with previous Zoo-FISH results with respect to comparison of conserved synteny. The eight, unique microsatellite markers developed from the physically assigned BACs positioned seven cytokine genes to corresponding positions on the linkage map, thus further integrating the bovine comprehensive map. These microsatellite markers provide a resource for incorporating cytokine candidate genes into a genome wide analysis of resource cattle populations segregating for disease resistance and susceptibility traits.