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Title: COMPARATIVE MAPPING OF A REGION ON CHROMOSOME 10 CONTAINING QTL FOR REPRODUCTION IN SWINE

Author
item Nonneman, Danny - Dan
item Rohrer, Gary

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/25/2002
Publication Date: 2/1/2003
Citation: NONNEMAN, D.J., ROHRER, G.A. COMPARATIVE MAPPING OF A REGION ON CHROMOSOME 10 CONTAINING QTL FOR REPRODUCTION IN SWINE. ANIMAL GENETICS. 2003. v. 34. p. 42-46.

Interpretive Summary: Several QTL for important reproductive traits (age of puberty, ovulation rate, teat number and plasma FSH) have been identified on the long arm of porcine chromosome 10(SSC10q). This region of the porcine genome is homologous to human chromosome 10p. Identification of genes accounting for significant effects on economically important traits requires development of human and porcine comparative maps. We wanted to increase the density of known genes mapped in this region of the porcine chromosome. Six genes were added to this region of the porcine map. Gene order was highly conserved within these markers from centromere to telomere of porcine chromosome 10q, as compared to human chromosome 10p. Four genes (PIP5K2a, ITIH2, GAD2 and AKR1C2), which map under QTL for age at puberty, plasma FSH and ovulation rate, are potential candidate genes. The identification of porcine homologues near important QTL and development of a comparative map for this chromosome will allow further fine-mapping and positional cloning of candidate genes responsible for reproductive traits.

Technical Abstract: Several quantitative trait loci (QTL) for important reproductive traits (age of puberty, ovulation rate, nipple number and plasma FSH) have been identified on the long arm of porcine chromosome 10. Bi-directional chromosome painting has shown that this region is homologous to human chromosome 10p. Because few microsatellite or type I markers have been placed on SSC10, we wanted to increase the density of known ESTs mapped in this region of the porcine genome. Genes were chosen for their position on human chromosome 10, sequence availability from the TIGR pig gene indices, and their potential as a candidate gene. PCR primers were designed to amplify across introns or 3'-UTR to maximize SNP discovery. Parents of the mapping population (one sire and seven dams) were amplified and sequenced to find informative markers. SNPs were genotyped using primer extension and mass spectrometry. These amplification products were also used to probe a BAC library (RPCI-44) for positive clones and screened for microsatellites. Six genes from human chromosome 10p (AKR1C2, PRKCQ, ITIH2, ATP5C1, PIP5K2A, and GAD2) were mapped in the MARC swine mapping population. Gene order was conserved within these markers from centromere to telomere of porcine chromosome 10q, as compared to human chromosome 10p. Four of these genes (PIP5K2a, ITIH2, GAD2, and AKR1C2), which map under QTL are potential candidate genes. Identification of porcine homologues near important QTL and development of a comparative map for this chromosome will allow further fine-mapping and positional cloning of candidate genes affecting reproductive traits.