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ARS Home » Pacific West Area » Logan, Utah » Forage and Range Research » Research » Publications at this Location » Publication #181953
Title: IDENTIFICATION OF HEDYSARUM VARIETIES USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP) ON A CAPILLARY ELECTROPHORESIS SYSTEM

Author
item KARUDAPURAM, SUREKHA - APPLIED BIOSYSTEMS, CA
item Larson, Steven

Submitted to: Journal of Biomolecular Techniques
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/15/2005
Publication Date: 12/1/2005
Citation: Karudapuram, S., Larson, S.R. 2005. Identification of hedysarum varieties using amplified fragment length polymorphism (aflp) on a capillary electrophoresis system. Journal of Biomolecular Techniques.

Interpretive Summary: For non-model organisms that do not have sequence information readily available AFLP is a well-established DNA fingerprinting technique that can be used for genetic analysis for a wide variety of organisms. While AFLP can be performed on a variety of instrument systems, the availability of a more automated system that integrates the assay and analysis to greatly increase their throughput capacity and facilitate routine AFLP analysis. We demonstrate the use of such a system for AFLP analysis on natural germplasm sources Hedysarum legumes from western North America. AFLP assays performed on samples belonging to two different species isolated from Utah identified different varieties that clustered as expected from their collection sites.

Technical Abstract: For non-model organisms that do not have sequence information readily available AFLP is a well-established technique that can be used for genomic mapping applications such as genetic diversity studies or phylogenetic studies. While AFLP can be performed on a variety of systems including gel-based systems that require multiple labor-intensive steps, the availability of a more automated system that integrates the assay, electrophoresis platform and analysis software could enable researchers to greatly increase their throughput and facilitate routine AFLP analysis. We demonstrate the use of such a system for AFLP analysis on Hedysarum species. AFLP assays performed on samples belonging to two different species isolated from Utah identified different varieties that clustered as expected from their actual locations.