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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #190592
Title: DEVELOPMENT AND CHARACTERIZATION OF KATY DELETION MUTANT POPULATIONS

Author
item Jia, Yulin
item XI, JIANKUN - JIANGXI ACADEMY OF AG. SC
item Rutger, J

Submitted to: Rice Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2005
Publication Date: 1/1/2006
Citation: Jia, Y., Xi, J., Rutger, J.N. 2006. Development and characterization of Katy deletion mutant populations. Plant Mutation Reports. 1(1):43-47.

Interpretive Summary: Rice is an important food, and is also an excellent model organism to study the function of rice genes, i.e., functional genomics. The genetic material suitable for functional genomics largely is from foreign countries where major rice is produced. It will take years to import these genetic stocks due to the restrictions of quarantine and intellectual property. To facilitate functional genomics studies and rice improvement, the US rice cultivar Katy was mutagenized by ethyl methane sulfonate (EMS), fast neutrons and gamma irradiations in cooperation with Jiangxi Agricultural Academy for Agricultural Science. Seeds were soaked in 0.4%, 0.8% and 1.2 % EMS solutions for 6 hours and a total of 7199 M1lines were recovered. Seven dosages of fast neutron were applied and a total of 15400 M1 lines were recovered from treatments with 7.7 Gy, 26.3 Gy and 49.4 Gy. Preliminary analysis of M2 seedlings revealed defects in chlorophyll synthesis in a range of 1.06% - 5.04% of M1 derived lines. Katy containing the blast resistance gene Pi-ta prevents infection by Magnaporthe grisea races in a gene for gene manner. M. grisea races containing the avirulence gene AVR-Pita are being used to identify susceptible mutants and M. grisea races lacking AVR-Pita are being used to screen resistant mutants. Sekiguchi lesion mimic-like mutants of Katy were recovered from lines induced by EMS, fast neutrons and gamma irradiations. Progress in genetic analysis and advancement of these putative mutant lines are described. All resulting mutants and genetic materials will be deposited in the Genetic Stock Oryza (GSOR) collection at Dale Bumpers National Rice Research Center.

Technical Abstract: To facilitate functional genomics studies and rice improvement, the US rice cultivar Katy was mutagenized by ethyl methane sulfonate (EMS), fast neutrons and gamma irradiations in cooperation with Jiangxi Agricultural Academy for Agricultural Science. Seeds were soaked in 0.4%, 0.8% and 1.2 % EMS solutions for 6 hours and a total of 7199 M1lines were recovered. Seven dosages of fast neutron were applied and a total of 15400 M1 lines were recovered from treatments with 7.7 Gy, 26.3 Gy and 49.4 Gy. Preliminary analysis of M2 seedlings revealed defects in chlorophyll synthesis in a range of 1.06% - 5.04% of M1 derived lines. Katy containing the blast resistance gene Pi-ta prevents infection by Magnaporthe grisea races in a gene for gene manner. M. grisea races containing the avirulence gene AVR-Pita are being used to identify susceptible mutants and M. grisea races lacking AVR-Pita are being used to screen resistant mutants. Sekiguchi lesion mimic-like mutants of Katy were recovered from lines induced by EMS, fast neutrons and gamma irradiations. Progress in genetic analysis and advancement of these putative mutant lines are described.