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ARS Home » Pacific West Area » Logan, Utah » Forage and Range Research » Research » Publications at this Location » Publication #212806
Title: Transcript Differences Associated With Non-Acclimated Freezing Tolerance in Two Barley (Hordeum Vulgare L.) Cultivars

Author
item KOO, B - NATIONAL INST CROP SCI
item Bushman, Shaun
item Mott, Ivan

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2008
Publication Date: 7/1/2008
Citation: Koo, B.C., Bushman, B.S., Mott, I.W. 2008. Transcript associated with non-acclimated freezing response in two barley cultivars. Plant Gen. 1:21-32

Interpretive Summary: Barley periodically suffers from late spring freezes in areas throughout the world, with significant losses to yield. To better understand the response of barley to spring freezes, we examined the response of Dicktoo and Keunal barley varieties in their jointing stage to non-acclimated freezing (NAF) at -3 degrees C; and the transcriptome response coincident with the same freezing treatment. Dicktoo was tolerant of NAF freezing for a longer period of time than Keunal, and had substantially more microarray probesets up-and down-regulated under NAF treatment with respect to controls. The transcriptome response of both Dicktoo and Keunal to NAF also differed from acclimation based freezing treatments. As candidate genes for tolerance to NAF, 106 probesets were selected, of which 44 had homology-based annotations. Transcription factors, circadian related genes, and other genes involved in photoperiod response were represented among the probesets up-regulated in Dicktoo NAF compared to Keunal NAF, while genes involved in RNA metabolism and water and sugar transport were down-related. Probesets up-regulated in Dicktoo NAF compared to Keunal NAF, were also up-regulated in Dicktoo control compared to Keunal control, suggesting a transcription state that was better prepared for freezing stress.

Technical Abstract: Barley periodically suffers from late spring freezes in area throughout the world, with significant losses to yield. To better understand the response of barley to spring freezes, we examined the response of Dicktoo and Keunal barley varieties in their jointing stage to non-acclimated freezing (NAF) at -3 degrees C; and the transcriptome response coincident with the same freezing treatment. Dicktoo was tolerant of NAF freezing for a longer period of time than Keunal, and had substantially more microarray probesets up-and down-regulated under NAF treatment with respect to controls. The transcriptome response of both Dicktoo and Keunal to NAF also differed from acclimation based freezing treatments. As candidate genes for tolerance to NAF, 106 probesets were selected, of which 44 had homology-based annotations. Transcription factors, circadian related genes, and other genes involved in photoperiod response were represented among the probesets up-regulated in Dicktoo NAF compared to Keunal NAF, while genes involved in RNA metabolism and water and sugar transport were down-regulated. Probesets up-regulated in Dicktoo NAF compared to Keunal NAF, were also up-regulated in Dicktoo control compared to Keunal control, suggesting a transcription state that was better prepared for freezing stress.