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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #218255
Title: Identification of SSR markers for a broad-spectrum blast resistance gene Pi-20(t) for marker-assisted breeding

Author
item LI, WEI - CHINESE ACAD. AGRIC. SCI
item LEI, CAILIN - CHINESE ACAD. AGRIC. SCI
item CHENG, ZHIJUN - CHINESE ACAD. AGRIC. SCI
item Jia, Yulin
item HUANG, DONGYI - CHINESE ACAD. AGRIC. SCI
item LIU, ZHANLING - CHINESE ACAD. AGRIC. SCI
item WANG, JIULIN - CHINESE ACAD. AGRIC. SCI
item SHI, KE - CHINESE ACAD. AGRIC. SCI
item ZHANG, XIN - CHINESE ACAD. AGRIC. SCI
item SU, NING - CHINESE ACAD. AGRIC. SCI
item GUO, XIUPING - CHINESE ACAD. AGRIC. SCI
item ZHAI, HUQU - CHINESE ACAD. AGRIC. SCI
item WAN, JIANMIN - CHINESE ACAD. AGRIC. SCI

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/29/2008
Publication Date: 4/19/2008
Citation: Li, W., Lei, C., Cheng, Z., Jia, Y., Huang, D., Liu, Z., Wang, J., Shi, K., Zhang, X., Su, N., Guo, X., Zhai, H., Wan, J. 2008. Identification of SSR markers for a broad-spectrum blast resistance gene Pi-20(t) for marker-assisted breeding. Molecular Breeding. 22:141-149.

Interpretive Summary:

Technical Abstract: The Pi-20(t) gene in rice confers broad-spectrum resistance against diverse pathotypes (races) of Magnaporthe oryzae (formerly Magnaporthe grisea) in China. Two flanking, and three co-segregating SSR markers nearby the centromere region of chromosome 12 for Pi-20(t) were identified using 526 extremely susceptible F2 plants derived from a cross of Asominori, an extremely susceptible cultivar with a highly resistant cultivar, IR24. OSR32 was mapped at 0.2cM of Pi-20(t) at one side and RM28050 was mapped at 0.4cM of Pi-20(t) at another side. Three SSR markers (RM1337, RM5364 and RM7102) were found to co-segregate with Pi-20(t). RM1337 and RM5364 were found to be reliable markers to tag resistance conditioned by Pi-20(t) in a wide range of elite rice germplasm in China. Thus, these two SSR markers are useful in marker-assisted incorporation of Pi-20(t) into advanced breeding lines for achieving durable and broad spectra of resistance to M. oryaze.