Development of rice materials for studying the genomics of disease resistance

Authors: Jia, Yulin; Lin, Michael; LIU, GUANGJIE - UNIV. OF AR RREC; McClung, Anna

Submitted to: American Phytopathology Society
Publication Type: Proceedings
Publication Acceptance Date: 4/15/2007
Publication Date: 7/29/2007
Citation: Jia, Y., Lin, M.J., Liu, G., Mcclung, A.M. 2007. Development of rice materials for studying the genomics of disease resistance. [abstract] American Phytopathology Society, 97:S52.

Interpretive Summary:

Technical Abstract: The availability of the rice genome sequence allows rapid characterization of resistance genes using standard methods of reverse and forward genetics. To facilitate genomic characterization of rice resistance genes a large mutant population based on a single rice genotype, Oryzae sativa cultivar 'Katy' (resistant to blast), was developed using a combination of chemicals, fast neutrons and gamma irradiations. To date 15,000 M4 have been recovered from the fast neutron treatment. An additional 25,000 M2 recovered from EMS and gamma irradiations are being advanced to M4 using the single seed breeding method. In addition, several recombinant inbred line populations with parents containing different resistance genes are being developed using the single seed descent method. These matierals will be deposited at the Genetic Stock-Oryzae collection (GSOR)(http://ars.usda.gov/Main/docs.htm?docid=8318) at Dale Bumpers National Rice Research Center, Stuttgart, AR. Currently 15,000 M4 of Katy and the RIL populations of the crosses of Raminade strain 3 (highly resistant to blast) with an experimental line, RU9101001 (highly susceptible), and Katy with RU9101001, Lemont (susceptible to sheath blight) and Jasmine 85 (resistant to sheath blight) are ready for distribution by contacting Yulin Jia at [e-mail].