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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #249844
Title: Preliminary Investigation of Origanum onites Essential Oil by Overpressured Layer Chromatography and BioArena

Author
item MINCSOVICS, EMIL - Oplc-Nit, Ltd
item Tabanca, Nurhayat
item MORICZ, AGNES - Hungarian Academy Of Sciences
item Wedge, David
item TYIHAK, EMO - Hungarian Academy Of Sciences

Submitted to: Planar Chromatography
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2010
Publication Date: 6/1/2010
Citation: Mincsovics, E., Tabanca, N., Moricz, A.M., Wedge, D.E., Tyihak, E. 2010. Preliminary Investigation of Origanum onites Essential Oil by Overpressured Layer Chromatography and BioArena. Planar Chromatography. 23:225-226.

Interpretive Summary: Essential oil from Turkish Oregano was chemically separated using Overpressured Layer Chromatography and subsequently evaluated for antimicrobial activity using BioArena system against Bacillus subtilis. This study demonstrated that OPLC coupled with direct bioautography is a powerful tool to separate and determine bioactive compounds in non-polar and polar complex mixtures.

Technical Abstract: Origanum is a genus of about 20 species of aromatic herbs in the family Lamiaceae, native to the Mediterranean region east to eastern Asia. The genus includes some important culinary herbs, including Marjoram and Oregano. Origanum onites (Turkish Oregano) oil and its major compounds were separated using Overpressured Layer Chromatography and subsequently evaluated for antimicrobial activity using BioArena system against Bacillus subtilis. This study demonstrated that OPLC coupled with direct bioautography is a powerful tool to separate and determine bioactive compounds in non-polar and polar complex mixtures.