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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #267237
Title: Analytical methods for determination of alkaloids and saponins from roots of Caulophyllum thalictroids (L) Michx using UPLC HPLC and HPTLC

Author
item AVULA, BHARATHI - University Of Mississippi
item WANG, YAN-HONG - University Of Mississippi
item RUMALLA, CHIDANANDA - University Of Mississippi
item ALI, ZULFIQAR - University Of Mississippi
item SMILLIE, TROY - University Of Mississippi
item KHAN, IKHLAS - University Of Mississippi

Submitted to: Journal of Pharmaceutical and Biomedical Analysis
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/22/2011
Publication Date: 7/26/2011
Citation: Avula, B., Wang, Y., Rumalla, C.S., Ali, Z., Smillie, T.J., Khan, I.A. 2011. Analytical methods for determination of alkaloids and saponins from roots of Caulophyllum thalictroids (L) Michx using UPLC HPLC and HPTLC. Journal of Pharmaceutical and Biomedical Analysis. 56:895-903.

Interpretive Summary: The newly developed method for chemical analysis of nine compounds was found to be less time consuming as compared to conventional methods. The method is suitable for chemical fingerprint analysis of alkaloids & saponins from samples of C. thalictroides, a botanical supplement. The profiles generated by different methods of plant samples and dietary supplements were compared for the presence of alkaloids and saponins. Because of the simple sample preparation, short analysis time and high reproducibility, the methods presented in this paper can be a useful tool for routine analysis of alkaloid and saponins from plant samples as well as commercial preparations and also helpful in assuring safety and quality control of blue cohosh preparations.

Technical Abstract: A comparison study of analytical methods including HPLC, UPLC and HPTLC are presented in this paper for the determination of major alkaloid and triterpene saponins from the roots of Caulophyllum thalictroides (L.) Michx. (blue cohosh) and dietary supplements claiming to contain blue cohosh. The methods were also successfully applied to analyze different dietary supplements. LC-mass spectrometry coupled with electrospray ionization (ESI) method is described for the identification and confirmation of nine compounds in various plant samples and dietary products. A HPTLC method was also developed for the fast chemical fingerprint analysis of C. thalictroides samples.