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Title: Enterobacteriaceae and salmonella recovered from non-sanitized and sanitized broiler hatching eggs

Author
item Musgrove, Michael
item STEPHENS, C - University Of Georgia
item Cox Jr, Nelson
item RICHARDSON, L. - Former ARS Employee
item MAULDIN, J. - University Of Georgia
item Buhr, Richard - Jeff

Submitted to: Poultry Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/16/2012
Publication Date: 7/9/2012
Citation: Musgrove, M.T., Stephens, C.B., Cox Jr, N.A., Richardson, L.J., Mauldin, J.M., Buhr, R.J. 2012. Enterobacteriaceae and salmonella recovered from non-sanitized and sanitized broiler hatching eggs [abstract]. Poultry Science. 91:(Supp. 1)P285. p. 97-98.

Interpretive Summary:

Technical Abstract: Inhibiting Salmonella contamination of hatching eggs could reduce the chance of broiler chicks becoming colonized during incubation and hatching. An experiment was conducted to determine the efficacy of a sanitizer (1,200 ppm quaternary ammonium- biguanide compound) applied as foam or spray in reducing Enterobacteriaceae or Salmonella from broiler hatching eggs. Eggs were treated prior to setting and at transfer. Two buggies (5040 eggs/buggy) were treated during each of four repetitions; two with spray, two with foam. Buggies of untreated eggs were used as controls. From each buggy, 20 eggs were collected aseptically into individual sterile bags and transported to the laboratory. Sampling was conducted by two methods: shell rinse and shell crush. Each egg was rinsed in bag with 20 mL phosphate buffered saline for 1 min or shells and membranes were placed in tubes with 20 mL PBS and macerated for 1 min using a sterile glass rod. A one mL portion of each sample was pour-plated using violet red bile glucose agar with an overlay. After incubation at 37oC for 18-24 h, positive samples were recorded and colonies were counted and converted to log10 cfu Enterobacteriaceae/mL sample. A pool of ten eggs rinsed or crushed was enriched for Salmonella. Each pooled sample was pre-enriched in buffered peptone water, selectively enriched in Rappaport-Vassiliadis and TT broth, and plated onto BGS and XLT-4 agar plates. All incubations were overnight at 37oC except for selective enrichment (42oC). Samples with presumptive colonies on either selective plate were further characterized by inoculation of TSI and LIA slants. Salmonella identifications were confirmed serologically and sero-grouped. Enterobacteriaceae (EB) counts were too low for accurate enumeration. Combing both reps, foamed or control eggs were set 0 and 12.5 % of EB positive at set, 10% and 30% at transfer. For sprayed or controls 5 and 22.5% were EB positive at set and 40% and 30% were positive at transfer. Salmonella was only recovered at transfer: Three of the foam treated and one foam control; and a single spray control. Only sero-groups B and C1 were recovered. Though neither application affected Salmonella prevalence, in terms of decreasing EB prevalence, foam application was effective at setting and transfer while spray application was effective at setting only.