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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #296850
Title: Development of practical diagnostic methods for monitoring rice bacterial panicle blight disease and evaluation of rice germplasm for resistance

Author
item WAMISHE, YESHI - University Of Arkansas
item Jia, Yulin
item KELSEY, CHRISTY - University Of Arkansas
item BELMAR, SCOTT - University Of Arkansas
item RASHEED, MAMNUR - University Of Arkansas

Submitted to: Arkansas Agricultural Experiment Station Research Series
Publication Type: Experiment Station
Publication Acceptance Date: 5/14/2013
Publication Date: 8/1/2014
Citation: Wamishe, Y., Jia, Y., Kelsey, C., Belmar, S., Rasheed, M. 2014. Development of practical diagnostic methods for monitoring rice bacterial panicle blight disease and evaluation of rice germplasm for resistance. In BR Wells Studies- series 609 2013. Edited by Norman, RJ., Molderhauer, KAK, University of Arkansas. Arkansas Agricultural Experiment Station Research Series. Pages 103-108.

Interpretive Summary:

Technical Abstract: A study was initiated to understand Burkholderia glumae, the major causal agent for bacterial panicle blight disease of rice; to develop practical diagnostic methods for monitoring the disease; and to evaluate rice germplasm for resistance. Burkholderia glumae was frequently isolated from infected panicles on semi-selective medium, des- ignated CCNT. Selected isolates were assessed for virulence by using a hypersensitivity reaction on wild tobacco leaves and pathogenicity tests on rice seedlings. B. glumae isolates found positive for tests in hypersensitivity and pathogenicity were used to in- oculate seedlings and adult rice plants (at heading) in the greenhouse, and germplasm screening for resistance in the field. Three hundred entries from the Uniform Regional Rice Nursery (URRN) and Arkansas Rice Performance Trials (ARPT) were inoculated between the boot-split and flowering growth stages of rice. Of 300 entries, 14 entries showed no symptom of the disease and 30 entries showed moderate resistance. These entries rated from 1 to 4 on a disease scoring scale of 0 to 9 where 0 is no disease and 9 is severe bacterial panicle blight disease. The remaining entries rated between 5 and 9 and were grouped as moderately susceptible to very susceptible. Greenhouse inoculation on adult rice plants at heading showed discoloration on a few florets in the susceptible variety Bengal. None of the seedling inoculations were definitive enough to separate relative resistance levels among the varieties tested. This study reports preliminary findings only from one season and additional tests, including molecular markers will continue for two more years.