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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #306788
Title: Identification of major blast resistance genes in the southern US

Author
item WANG, XUEYAN - University Of Arkansas
item WAMISHE, YESHI - University Of Arkansas
item Bianco, Tracy
item Lin, Michael
item VALENT, BARBARA - Kansas State University
item Jia, Yulin

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2014
Publication Date: 8/1/2014
Citation: Wang, X., Wamishe, Y., Bianco, T.A., Lin, M.J., Valent, B., Jia, Y. 2014. Identification of major blast resistance genes in the southern US. American Phytopathological Society Annual Meeting, August 9-13, 2014, Minneapolis, MN. O68.

Interpretive Summary:

Technical Abstract: Resistance (R) genes in rice play important roles in preventing infections of rice blast fungus, Magnaporthe oryzae. In order to identify more R genes for different rice growing areas in the Southern US, an extensive field survey of the blast fungus was performed from 2012 to 2013. A total of 500 isolates were purified from diseased rice leaves, panicles, and grains from commercial fields and experimental stations in Arkansas, Louisiana, Missouri, and Texas. A total of 100 isolates were selected based on geographical location and host genotype for race identification using international rice differentials with the addition of Katy (with Pita/Pita2) and M202 (without Pita/Pita2). Based on race identity, 20 isolates were selected for sequencing to examine the alleles of avirulence (AVR) genes including AVR-Pita1, AVR-Pi9, and AVR-Pik. The same isolates were used to inoculate monogenic rice lines carrying 26 major blast R genes developed by the International Rice Research Institute, the Philippines. Results of DNA sequence analysis of these AVR genes and pathogenicity assays were combined to identify additional effective blast R genes for different rice production regions in the Southern US.