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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Endemic Poultry Viral Diseases Research » Research » Publications at this Location » Publication #312816
Title: Reconstitution of infectious laryngotracheitis from a collection of overlapping cosmid clones

Author
item Spatz, Stephen
item GARCIA, MARICARMEN - University Of Georgia
item RIBLET, SYLVIA - University Of Georgia
item Ross, Teresa

Submitted to: Western Poultry Disease Conference
Publication Type: Proceedings
Publication Acceptance Date: 12/7/2014
Publication Date: 3/22/2015
Citation: Spatz, S.J., Garcia, M., Riblet, S., Ross, T.A. 2015. Reconstitution of infectious laryngotracheitis from a collection of overlapping cosmid clones. In: Proceedings of the Sixty-Fourth Western Poultry Disease Conference, March 22-25, 2015, Sacramento, California. p. 134-138.

Interpretive Summary:

Technical Abstract: We have generated overlapping cosmids that span the complete genome of infectious laryngotracheitis virus (ILTV) and have used these clones in transfection experiments to reconstitute the virus. This is the first example of the use of large deoxyribose nucleic acid fragment(s) (cosmid, fosmid, bacterial artificial chromosomes and yeast artificial chromosomes) to generate an infectious clone of ILTV. This is of significance since mutations can be easily introduced within the ILTV genome using the recombination machinery of E.coli.