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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #327751
Title: Molecular screening of Magnaporthe Oryzae avirulence genes

Author
item UNAN, RASIM - Trakya University
item Jia, Yulin

Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 4/13/2016
Publication Date: 4/13/2016
Citation: Unan, R., Jia, Y. 2016. Molecular screening of Magnaporthe Oryzae avirulence genes. Fungal Genetics Conference Proceedings. Paris, France, April 3-6, 2016.

Interpretive Summary:

Technical Abstract: Rice blast disease caused by Magnaporthe oryzae is one of the most destructive diseases of rice, which has been threatening rice production worldwide. The avirulence (AVR) genes of M. oryzae triggers race-specific resistance when strains of M. oryzae infect rice cultivars that contain the corresponding resistance genes. Screening AVR genes of M. oryzae is vital for selecting effective host resistance genes. We evaluated 117 isolates of M. oryzae collected from rice fields of the Southern USA including Arkansas, Louisiana, Mississippi, and Texas in 2013 and 2014. Eight AVR genes were screened with PCR using AVR-Pita1, AVR-PiK, AVR-Pizt, AVR-Pia, AVR-Pii, AVR-Pi15, AVR-Pi, and AVR-Pit specific primers. These AVR gene specific primers can amplify DNA fragment of the coding region in addition to 5’ leader and 3’ trailer regions of each AVR gene. The frequency of amplification of AVR-Pizt, AVR-Pi7, AVR-Pita1, AVR-PiK, AVR-Pia, AVR-Pii, AVR-Pi15, and AVR-Pit was 88.8%, 78.6%, 64.1%, 21.3%, 3.4%, 2.6%, 0.9%, and 0.0%, respectively. These findings suggest that Pi-zt, Pi7, Pi-ta, and Pi-K are effective resistance genes for deployment in rice growing areas where 117 field blast strains were collected.