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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #330404
Title: Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome

Author
item LEE, JONG-YEOL - National Institute For Agricultural Science & Technology
item KANG, CHON-SIK - National Institute For Agricultural Science & Technology
item BEOM, HYE-RANG - National Institute For Agricultural Science & Technology
item JANG, YOU-RAN - National Institute For Agricultural Science & Technology
item Altenbach, Susan
item LIM, SUN-HYUNG - National Institute For Agricultural Science & Technology
item KIM, YOUNG-MI - National Institute For Agricultural Science & Technology
item PARK, CHUL-SOO - Chonbuk National University

Submitted to: Journal of Cereal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/15/2016
Publication Date: 12/18/2016
Citation: Lee, J., Kang, C., Beom, H., Jang, Y., Altenbach, S.B., Lim, S., Kim, Y., Park, C. 2016. Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome. Journal of Cereal Science. 73:158-164.

Interpretive Summary: The manuscript describes the molecular characterization of a novel wheat line called DH20 that was obtained by crossing two Korean wheat cultivars Keumkang and Olgeuru. Flour from the new line is missing a number of gluten proteins that have been considered to be important for bread-making quality. . DH20 and the parental lines were grown in field plots during two consecutive years and comparisons were made of their agronomic and bread-making properties. DH20 had higher average grain yields than either of its parents. DH20 was similar to the Keumkang parent in dough mixing properties, but produced smaller bread loaves with a firmer crumb. The data indicate that DH20 should be valuable for future studies aimed at understanding the roles of the specific gluten proteins in bread-making quality.

Technical Abstract: DH20, a new wheat mutant missing low-molecular weight glutenin subunits encoded by the Glu-B3 locus, was discovered among double haploid lines obtained from a cross between the Korean wheat cultivars Keumkang and Olgeuru. Absence of the Glu-B3 LMW-GS proteins was determined by one-dimensional gel electrophoresis (SDS-PAGE) and confirmed by two-dimensional gel electrophoresis (2-DGE) and tandem mass spectrometry (MS/MS). The deletion of Glu-B3 genes was also demonstrated using allele specific DNA markers. Basic agronomic traits, protein content, dough mixing properties and bread loaf volume of DH20 and parental wheat cultivars were evaluated in field-grown wheat over a two year period. This mutant is a valuable resource for understanding the function of individual LMW-GS alleles or genes at the Glu-B3 locus using either breeding or biotechnology approaches.