Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products

Authors: HU, L - Northeast Agricultural University; MA, L - Oklahoma State University; ZHENG, S - Northeast Agricultural University; He, Xiaohua; WANG, H - Food And Drug Administration(FDA); BROWN, E. W. - Food And Drug Administration(FDA); HAMMACK, T. S. - Food And Drug Administration(FDA); ZHANG, G. D. - Food And Drug Administration(FDA)

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/13/2016
Publication Date: 11/2/2016
Publication URL: https://handle.nal.usda.gov/10113/5700691
Citation: Hu, L., Ma, L., Zheng, S., He, X., Wang, H., Brown, E., Hammack, T., Zhang, G. 2016. Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products. Poultry Science. 96(5):1410-1418. doi: 10.3382/ps/pew399.

Interpretive Summary: Every year salmonella is estimated to cause one million foodborne illnesses in the United States, with 19,000 hospitalizations and 380 deaths. Death caused by Salmonella can be avoided if the person is treated promptly with antibiotics. Therefore, effective diagnostic methods that is simple, fast, affordable and could be used on-site are important. This manuscript evaluates a new technology called Loop-mediated isothermal amplification (LAMP) for detection of Salmonella in egg products. Two LAMP systems, MDS and PDS, were compared to FDA’s BAM manual. Four Salmonella isolates in 7 wet and 13 dry egg products were examined. It was found that results obtained by MDS and PDS were 100% in agreement with BAM method using buffered peptone water (BPW) as pre-enrichment broth. When LB was used as pre-enrichment broth, the BAM, MDS, and PDS methods respectively detected 63%, 61%, and 60% of Salmonella in 80 tested samples. This study suggests that both MDS and PDS are as effective as BAM and can be used as rapid detection tools for Salmonella in egg products.

Technical Abstract: Loop-mediated isothermal amplification (LAMP) is a novel simple detection technology that amplifies DNA with high speed, efficiency, and specificity under isothermal conditions. The objective of this study was to evaluate the effectiveness of 3M Molecular Detection System (MDS) and ANSR Pathogen Detection System (PDS) for detecting Salmonella from egg products in comparison with Food and Drug Administration Bacteriological Analytical Manual (BAM) culture method and a modified culture method (LAMP preferred method). Two Salmonella ser. Enteritidis (18579, PT4; CDC_2010K_1441, PT8), one Salmonella ser. Heidelberg (607310-1), and one Salmonella ser. Typhimurium (0723) isolates were used in this study. Seven wet egg products and thirteen dry egg products were inoculated with these strains individually at 1 - 5 CFU/25 g. One set of samples were prepared following FDA BAM procedures [with Lactose broth (LB) as pre-enrichment broth]. Another set of samples were prepared using buffered peptone water (BPW) as pre-enrichment broth as instructed by the two LAMP detection kits. The 3M MDS and ANSR PDS were 100% in agreement with their BPW based culture method results. When LB was used as pre-enrichment broth, the number of Salmonella positive test portions (80 tested), identified with the BAM, 3M MDS, and ANSR PDS, were, 63, 61 and 60, respectively. In conclusion, both 3M MDS and ANSR PDS Salmonella assays were as effective as their BPW based culture method for the detection of Salmonella from egg products. These sensitive LAMP assays could be used as rapid detection tools for Salmonella in egg products when BPW was used as pre-enrichment broth.