Fast neutron radiation induced Glu-B1 deficient lines of an elite bread wheat variety

Authors: L Chingcuanco, Debbie

Submitted to: Triticeae Genomics and Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2016
Publication Date: 4/12/2017
Citation: Chingcuanco, D.L. 2017. Fast neutron radiation induced Glu-B1 deficient lines of an elite bread wheat variety. Triticeae Genomics and Genetics. 8(1):1-8. https://doi.org/10.5376/tgg.2017.08.0001.
DOI: https://doi.org/10.5376/tgg.2017.08.0001

Interpretive Summary: The mixing properties of the dough are critical in the production of bread and other food products derived from wheat. The high molecular weight glutenin subunits (HMW-GS) are flour proteins that are major determinants of wheat dough processing qualities. Different HMW-GS differ in their effects on dough quality. To determine how this occurs, lines deficient in HMW-GS proteins were generated by fast-neutron radiation mutagenesis in a commercially grown bread wheat Triticum aestivum variety “Summit”. Five independent lines deficient in HMW-GS Bx17 and By18 proteins encoded by the Glu-B1 locus were identified by profiling the protein contents of seeds from the mutagenized population. Polymerase chain reaction gene primers designed to be specific for Bx17 and By18 showed that these genes were missing in these mutant lines. These lines will facilitate the development not only of different varieties of wheat with defined dough functionality for specific end-use but also varieties that could be safer to consume by people with gluten-sensitivities.

Technical Abstract: Five isogenic wheat lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded by the B-genome were identified from a fast-neutron radiation-mutagenized population of Summit, an elite variety of bread wheat (Triticum aestivum L.). The mutant lines differ from the wild-type progenitor by the absence of the HMW-GS Bx17 and By18 proteins. Gene specific primers designed for Bx17 and By18 genes failed to generate the diagnostic amplicons in the mutant lines. Assays using the iSelect Illumina Wheat 90K SNP arrays revealed that the induced deletions in these lines ranged from 5.58 to 127.47 cM. Isogenic wheat lines deficient in the different HMW-GS proteins will allow the determination of the contributions of these different genes to dough quality. This new germplasm will serve as genetic tools to generate healthier wheat varieties with altered dough performance for diverse end-uses.