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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #339676
Research Project: Antibiotic Alternatives for Controlling Foodborne Pathogens and Disease in Poultry

Location: Poultry Production and Product Safety Research

Title: Heat stability of carvacrol and trans-cinnamaldehyde during commercial manufacturing temperatures in chicken feed

Author
item UPADHYAYA, INDU - University Of Arkansas
item YIN, HSIN-BAI - University Of Connecticut
item GIDDEN, JENNIFER - University Of Arkansas
item LAY, JACKSON - University Of Arkansas
item Donoghue, Ann - Annie
item DONOGHUE, DAN - University Of Arkansas
item DARRE, MICHAEL - University Of Connecticut
item VENKITANARAYANAN, KUMAR - University Of Connecticut

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: With increasing consumer demand for natural, antibiotic free poultry products, the use of plant-derived antimicrobials (PDAs) as effective antimicrobial feed additives has gained significant attention. Among the various PDAs, carvacrol (CR) from oregano and trans-cinnamaldehyde (TC) from cinnamon bark have demonstrated significant antimicrobial properties in several chicken feeding trials. However, little is known about their stability in chicken feed subjected to commercial manufacturing temperatures and storage. This study investigated the heat stability of CR and TC in chicken feed subjected to two commercial heating temperatures and storage for 2 months. One kilogram of feed supplemented with or without (control) 0.75% CR or TC was heated at 150oF and 180oF. The heat-treated and untreated feed were stored at 25oC for 2 months. Immediately after treatment, and at 1 and 2 months of storage period, 100 gram portions of feed were analyzed for PDA content by Gas Chromatography Mass Spectrometry (GC-MS) on a Varian 450-GC coupled to a 320- MS triple quad mass spectrometer. Triplicate samples of each treatment and control were included, and data were analyzed using MS-review software. The peaks for each PDA were compared with oleic acid (endogenous control) for relative quantification. Carvacrol and TC were detected in the feed during the entire storage period. The levels of CR and TC in the feed heated at both temperatures were not affected as compared to the controls on day 0 (P>0.05). Similar results were observed at 1 and 2-month storage periods; heat-treated TC was stable at 1 and 2-month sampling points with no significant difference on storage (P>0.05). Although, CR in heat-treated and CR controls was detected by the end of 2 months, heat-treated samples on day 0 yielded greater peaks for CR when compared to 1 and 2 months of storage (P<0.05). This study suggests that PDAs, especially TC is heat stable for 2 months under commercial feed manufacturing temperatures, and could potentially be used as an effective antimicrobial additive in chicken feed.