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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #344907
Research Project: Non-Antibiotic Strategies to Control Priority Bacterial Infections in Swine

Location: Virus and Prion Research

Title: Vaccine development for protection against systemic infections with Streptococcus suis and Haemophilus parasuis in swine

Author
item Brockmeier, Susan
item EBERLE, K - Orise Fellow
item MOU, K - Orise Fellow
item HAU, S - Iowa State University
item Loving, Crystal
item Nicholson, Tracy
item WAACK, U - Orise Fellow

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/19/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Both Streptococcus suis and Haemophilus parasuis are important invasive bacterial pathogens of swine, commonly causing meningitis, arthritis, polyserositis, and septicemia. Due to the presence of many serotypes and high genotypic variability, efficacious vaccines are not readily available. We are using various strategies such as functional genomic screens, immunoproteomics, and attenuation to develop potential vaccine candidates, followed by testing against both homologous and heterologous protection against these pathogens. The selection of protein candidates for inclusion in vaccines is accomplished by identifying fitness genes through a functional genomics screen, selecting predicted surface-associated proteins, and identifying proteins conserved across isolates to enhance the prospect of cross-protection. Immunoproteomic methods are being used to detect proteins that are reactive with antisera from pigs that are more broadly protected compared to antisera from pigs only protected against homologous challenge in order to identify potential targets responsible for heterologous protection. Mutants in purported virulence factors are also being tested for attenuation and possible use as vaccines. Vaccine trials are examining the efficacy of these vaccine candidates against systemic disease caused by S. suis or H. parasuis. Through this rational approach we have identified several promising vaccine candidates against these important swine diseases.