Marinicella sediminis sp. nov., isolated from marine sediment

Authors: WANG, XIAO-QUN - Shandong University; LI, CHANG-MING - Shandong University; Dunlap, Christopher; Rooney, Alejandro - Alex; DU, ZONG-JUN - Shandong University

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/11/2018
Publication Date: 7/1/2018
Citation: Wang, X., Li, C., Dunlap, C.A., Rooney, A.P., Du, Z. 2018. Marinicella sediminis sp. nov., isolated from marine sediment. International Journal of Systematic and Evolutionary Microbiology. 68:2335-2339. https://doi.org/10.1099/ijsem.0.002839.
DOI: https://doi.org/10.1099/ijsem.0.002839

Interpretive Summary: ARS researchers from Peoria, IL collaborated with scientists from Shandong University in Weihai, China to characterize a novel bacterium found in marine environment. This manuscript reports on the characterization of a new species of bacteria Marinicella sediminis that grows in moderate salt concentrations. This species possesses novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications can be assessed in future studies.

Technical Abstract: A novel heterotrophic, Gram-stain-negative, aerobic, rod-shaped, pale yellow, non-motile and non-spore-forming bacterium, designated as strain F2**T, was isolated from the marine sediment collected from Weihai coastal, Shandong Province, PR China. Optimal growth occurred at 33 °C (range 10–37 °C), with 3–4% (w/v) NaCl (range 1–8%), and at pH 7.5–8.0 (range pH 6.5–9.0). Q-8 was the sole respiratory quinone. The major polar lipids of strain F2**T were phosphatidylethanolamine, one unidentified lipid, four unidentified glycolipids and four unidentified phospholipids. The major cellular fatty acids in strain F2**T was iso-C15:0 (46.6%). The genomic DNA G+C content of the strain was 48.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain F2**T is most closely related to Marinicella litoralis JCM 16154**T, with 16S rRNA gene sequence similarity of 97.5%; besides, Marinicella pacifica sw153**T with similarity of 96.0%. On the basis of the genome sequence data and physiological characteristics, we concluded that the strain F2**T was positioned within the Gammaproteobacteria and represents a novel species of the genus Marinicella, for which the name Marinicella sediminis sp. nov. is proposed. The type strain of the new species is F2**T (=KCTC 42953**T= MCCC 1H00149**T).