Skip to main content
ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #349994
Research Project: Development of Production and Formulation Technologies for Microbial Biopesticides in Conjunction with the Development of Attractants and Repellents for Invasive Insect Pests

Location: Crop Bioprotection Research

Title: Identifying Isaria javanica with high resolution DNA melting assays

Author
item Dunlap, Christopher

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/16/2018
Publication Date: 7/16/2018
Citation: Dunlap, C.A. 2018. Identifying Isaria javanica with high resolution DNA melting assays. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Isaria javanica is a commonly isolated entomopathogenic fungi with activity against many insect species. Over the years, strains of this species have been commonly misidentified as Isaria fumosorosea. We have developed a simple high resolution DNA melting assay to discriminate Isaria javanica from other Isaria species. The assay utilizes a previously identified 103 base pair PCR amplicon, which was reported to be selective for Isaria fumosorosea. Our study finds the amplicon selective for Isaria javanica and Isaria poprawskii, when assayed against all members of the genus. The practical application of this technique was confirmed using a bioassay on whitefly nymphs (Bemisia tabaci biotype B) inoculated with I. javanica.