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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Exotic & Emerging Avian Viral Diseases Research » Research » Publications at this Location » Publication #350527
Research Project: Intervention Strategies to Predict, Prevent and Control Disease Outbreaks Caused by Emerging Strains of Virulent Newcastle Disease Viruses

Location: Exotic & Emerging Avian Viral Diseases Research

Title: Immunization of chickens with an avian paramyxovirus 10 isolated from Rockhopper Penguins does not provide protection against challenge with virulent Newcastle disease virus

Author
item GORAICHUK, IRYNA - Consultant
item DIMITROV, KIRIL - Consultant
item SHARMA, POONAM - Food Safety Inspection Service (FSIS)
item Olivier, Timothy
item Miller, Patti
item Swayne, David
item Afonso, Claudio
item Suarez, David

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2018
Publication Date: 5/29/2018
Citation: Goraichuk, I.V., Dimitrov, K.M., Sharma, P., Olivier, T.L., Miller, P.J., Swayne, D.E., Afonso, C.L., Suarez, D.L. 2018. Immunization of chickens with an avian paramyxovirus 10 isolated from Rockhopper Penguins does not provide protection against challenge with virulent Newcastle disease virus [abstract]. 2018 International Avian Respiratory Disease Conference, Athens, Georgia, May 29-June 1, 2018.

Interpretive Summary:

Technical Abstract: Four viral isolates from Rockhopper Penguins were previously identified as members of a novel avian paramyxovirus serotype 10 (APMV-10). Whole genome random next-generation sequencing was performed and phylogenetic analysis showed that the isolates were most closely related to APMV-2 and APMV-8. Intracerebral pathogenicity index test indicated that the viruses are not virulent for chickens. One of the isolated viruses, APMV10-Rock-Hopper-Penguin-Falkland-Islands-539-2007, was further characterized in vivo. Three groups (I, II and III, n = 5 each) of 3-week-old SPF chickens were inoculated oculonasally with 102, 104, 106 EID50 of the virus per bird, respectively. At 2 days post inoculation (dpi) three additional SPF chickens were placed into each group to evaluate virus transmission. Serum samples were collected at 14 dpi. No chickens from group I seroconverted after inoculation with APMV-10, one and five directly inoculated chickens from groups II and III developed anti-APMV-10 antibodies as demonstrated by HI. No chickens showed any apparent clinical signs after inoculation with APMV-10. At 14 dpi the three contact chickens from group I and all inoculated chickens from groups II and III were challenged oculonasally with 106 EID50 per bird of virulent NDV-California-2002. Cloacal and oropharyngeal swabs were collected at 2 and 4 days post challenge (dpc). Clinical signs with 100% mortality were observed in all chickens after challenge with the virulent NDV strain. The APMV-10 virus was able to replicate in chickens at higher challenge doses, but without causing any clinical signs. Also, no cross protection against virulent NDV challenge was observed.