Release of a new set of lines of an elite variety of wheat with deletions in Glu-B1 Locus (GluB1-S60E)

Authors: L Chingcuanco, Debbie

Submitted to: Germplasm Release
Publication Type: Germplasm Registration
Publication Acceptance Date: 7/12/2018
Publication Date: 4/12/2017
Citation: Chingcuanco, D.L. 2017. Release of a new set of lines of an elite variety of wheat with deletions in Glu-B1 Locus (GluB1-S60E). Germplasm Release. 8(1):1-8.

Interpretive Summary:

Technical Abstract: GluB1-S60E wheat (Triticum aestivum L.) was developed by USDA-ARS at the Western Regional Research Center in Albany, CA. GluB1-S60E was identified from a fast-neutron radiation (FNR) mutagenized population of ‘Summit’, a commercial bread wheat variety developed by a small seed company Resource Seeds, Inc. (Goshen, CA) which was acquired by Syngenta in 2008. The progenitor Summit (PVP200200239) is a product of hybridization between ‘Express’ and ‘Tadorna’/’Proband775’. Express is a variety from Western Plant Breeders (Bozeman, MT), whereas Proband 775 is a variety from a company previously known as Northrup King, Co. (Minneapolis, MN). Tadorna is a Septoria leaf blotch (Septoria tritici) resistant line from the University of California, Davis. Summit expresses five HMW-GS genes: Ax1, Bx17+By18, Dx5+Dy10, considered as one of the best allelic pair combinations for bread baking. GluB1-S60E was identified by screening mutagenized M3 seeds for altered storage protein profiles using one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It is distinct from the wild-type progenitor line by the absence of the high molecular weight glutenin subunit (HMW-GS) Bx17 and By18 proteins. The Bx17+By18 protein deficiency in GlubB1-S60E is due to a 1.32 cM deletion in the long arm of chromosome 1B that includes the Glu-1B locus. The inheritance of the HMW-GS Bx17 and By18 protein deficiency phenotype of GluB1-S60E is stable as evidenced by its consistent transmission to progenies of plants grown in the greenhouse. The morphological and developmental phenotypes of greenhouse grown GluB1-S60E are similar with those of its wild type Summit progenitor. Briefly, GluB1-S60E is a hard-red spring common wheat with erect plant growth, anthocyanin-less hollow stem, mid-dense tapering head with awned spikelets and florets with yellow anthers. The wild-type Summit progenitor is tolerant to stripe rust, speckled leaf blotch, leaf rust, barley yellow dwarf virus, however, these traits have not yet been evaluated in GluB1-S60E wheat. The release is targeted for use by domestic and international wheat researchers and wheat breeders. Genetic variations in the HMW-GS loci correlate well with dough quality. Different allelic pair combinations in the HMW-GS gene loci vary in their effect on dough properties. The gluten proteins including those encoded in the GluB1-S60E locus are known to contain immunogenic epitopes that cause gluten-related health risks e.g. celiac disease. GluB1-S60E, together with other glutenin deficient lines, will be used to develop wheat varieties with altered seed protein composition with modified dough functionalities and to develop wheat-derived products with reduced immunogenic potential.