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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #369232
Research Project: Biology, Ecology, and Genomics of Pathogenic and Beneficial Microorganisms of Wheat, Barley, and Biofuel Brassicas

Location: Wheat Health, Genetics, and Quality Research

Title: Genetic and pathogenic variation in Heterodera latipons populations from Turkey

Author
item DABABAT, ABDELFATTAH - International Maize & Wheat Improvement Center (CIMMYT)
item DUMAN, NAGIHAN - Bolu Abant Izzet Baysal University
item OZER, GOKSEL - Bolu Abant Izzet Baysal University
item MOKRINI, FOUAD - National Institute Of Agronomic Research Of Morocco (INRA)
item IMREN, MUSTAFA - Bolu Abant Izzet Baysal University
item Paulitz, Timothy

Submitted to: Journal of Nematology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/6/2020
Publication Date: 6/1/2020
Citation: Dababat, A., Duman, N., Ozer, G., Mokrini, F., Imren, M., Paulitz, T.C. 2020. Genetic and pathogenic variation in Heterodera latipons populations from Turkey. Journal of Nematology. https://doi.org/10.1163/15685411-bja10029.
DOI: https://doi.org/10.1163/15685411-bja10029

Interpretive Summary: Mediterranean cereal cyst nematode, Heterodera latipons is an important plant parasite causing substantial yield loss in wheat throughout the world. Four provinces in Turkey were surveyed for this nematode. ITS sequencing was done on the collection and we found 4 populations. We also determined pathotypes (races) of the nematodes by planting on a defined set of differential varieties with different resistance (Cre) genes. This is the first report of pathotypes of H. latipons in Turkey.

Technical Abstract: The Mediterranean cereal cyst nematode, Heterodera latipons is an important plant parasite causing substantial yield loss in wheat throughout the world. It is considered one of the most economically damaging pathogens of wheat and barley in the southern part of Turkey. H. latipons exhibits high variation in its virulence and reproductive capacity even on the same host. This study aimed to characterize H. latipons populations from Hatay, Gaziantep, Kilis and Mardin provinces in Turkey based on their genetic variability and pathotypes. The populations were identified as H. latipons by sequencing the ITS-rDNA region and further sequence analysis showed an intraspecific genetic variation in H. latipons populations which were divided into four groups; Group I (Gaziantep population), Group II (Hatay population), Group III (Kilis the population), and Group IV (Mardin population). The differential host materials called “International Test Assortment” along with the international susceptible wheat cultivar ‘Seri-82’ were used to identify the pathotypes of the four populations. The results showed that the barley cultivars Ortolan, Morocco, Bajo Aragon 1-1, Herta, and Harlan were resistant or moderately resistant to Gaziantep, Kilis, and Mardin populations of H. latipons. Siri (Rha2) and KVL 191 (Rha2) were resistant to Hatay population, but they were susceptible to Gaziantep, Kilis and Mardin populations. The oat cultivars were resistant to the four tested populations. Among wheat cultivars, Capa and AUS 10894 (Cre1) were susceptible to the four nematode populations, while Iskamisch K-2-light and Psathias cultivars were resistant to all H. latipons populations except for Hatay population. Populations of Gaziantep, Kilis and Mardin exhibited the greatest identity to the Ha51 pathotype under the Ha2 group. This is the first report to identify H. latipons pathotypes in Turkey, which provides reference data for the breeding programs.