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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #370774
Research Project: Gene Discovery and Crop Design for Current and New Rice Management Practices and Market Opportunities

Location: Dale Bumpers National Rice Research Center

Title: Over expression of the candidate Pi-ta2 gene in rice (Oryza sativa L. japonica cv. Katy) for resistance against Blast Fungus (Magnaporthe oryzae)

Author
item CHHETRI, GAURAV THAPA - University Of Arkansas At Pine Bluff
item PONNIAH, SATHISH KUMAR - University Of Arkansas At Pine Bluff
item Jia, Yulin
item MANOHARAN, MUTHUSAMY - University Of Arkansas At Pine Bluff
item VALENT, BARBARA - Kansas State University

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/7/2020
Publication Date: 1/10/2020
Citation: Chhetri, G., Ponniah, S., Jia, Y., Manoharan, M., Valent, B. 2020. Over expression of the candidate Pi-ta2 gene in rice (Oryza sativa L. japonica cv. Katy) for resistance against Blast Fungus (Magnaporthe oryzae) [abstract]. Plant and Animal Genome Conference, San Diego, California, January 11-15, 2020.

Interpretive Summary:

Technical Abstract: Rice blast, caused by Magnaporthe oryzae is one of the most destructive diseases in rice, which feeds one-half of the world's population. An innate defense mechanism in plants known as effector triggered immunity (ETI) is mediated in rice by blast-resistance (R) genes most of which encode cytoplasmic proteins with nucleotide binding site-leucine-rich repeat (NLR) responsible for interacting with fungal effectors to trigger ETI. In the United States, breeding programs utilize a R gene cluster presumably carrying Pi-ta, Pi-ta2, and Ptr from the tropical japonica cultivar Katy to confer blast resistance. The objective of this study was to over express the candidate Pi-ta2 gene encoding a NLR protein at chromosome 12 in rice (LOC_Os12g18374) located in between Pi-ta and Ptr and understand its role in blast resistance. Plant transformation was carried out using 14-day old embryogenic calli cultured on N6D medium containing 3.0mg/L 2, 4-D. Calli were infected with Agrobacterium tumefaciens strain EHA105 carrying the binary plant expression vector pCAMBIA 1304 containing the candidate Pi-ta2 gene. A total of 66 hygromycin resistant calli, obtained from 174 infected ones, were placed on regeneration media containing 30mg/L hygromycin and 200mg/L timentin. Polymerase chain reaction (PCR) was carried out on twelve independent events regenerated from the rooting media using gene specific and hygromycin primers. The results confirmed the presence of respective genes in six plants of T0 generation. Expression analysis using quantitative real-time transcriptase PCR (qRT-PCR) and scoring after inoculation with blast fungus IB-49 (ML1) will be performed on T1 plants. Results will help to elucidate the molecular basis of evolutionary mechanisms of rice blast disease R genes.